Analysis of Antioxidant Effects of Lycopene and Caffeine in Experimentally Induced Renal Ischemia-Reperfusion Injury in Rats
Küçük Resim Yok
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Background: The aim was to investigate the antioxidant effects of lycopene and caffeine in rats exposed to renal ischemia/reperfusion (IR) injury. Materials and Methods: Following right renal nephrectomy, 60 minutes of ischemia followed by 60 minutes of reperfusion were performed in 200-250 gram Wistar albino rats used in the study. 30 minutes before ischemia, lycopene (5 mg/kg), caffeine (50 mg/kg), lycopene (5 mg/kg) + caffeine (50 mg/kg) were adminis- tered intraperitoneally to the rats. After the experiment, 4 ml of blood and left kidney were taken from the rats. Tissue malondialdehyde (MDA), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and se- rum creatinine activities were measured biochemically and light microscopy findings were evaluated histo- logically. Results: When MDA levels are compared; Although the MDA level of the IR+Lycopene group was lower than the IR group, no significance was observed. When IR+Lycopene and IR+Caffeine groups are compared; The MDA level of the IR+Lycopene group is higher than the IR+ Caffeine group. In the IR+Caffeine group; A sta- tistically significant increase was observed in serum creatinine compared to the IR+Lycopene+Caffeine group. Although SOD and GSH-Px were higher in the antioxidant groups compared to the IR group, no sta- tistically significant difference was observed. When compared with ischemia-reperfusion group, histopatho- logical damage was found to be significantly lower than in the other groups. It was found that Lycopene and Caffeine reduced apoptosis, tubular dilatation, tubular epithelium degeneration, glomerular shrinkage, des- quame epithelium and proteinous cast amount significantly. Conclusions: While lycopene has high antioxidant efficiency when compared with caffeine, lycopene and caffeine have protective effect against renal ischemia reperfusion injury.
Açıklama
Anahtar Kelimeler
Kidney, Ischemia/Reperfusion Injury, Lycopene, Caffeine
Kaynak
Harran Üniversitesi Tıp Fakültesi Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
18
Sayı
3
