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Öğe The effect of protocatechuic acid on ovarian histopathology and reserve in rat ovarian torsion model(AEPRESS SRO, 2023) Erimşah, Sevilay; Çetinkaya, AyhanOBJECTIVES: The aim of the study is to investigate the effects of Protocatechuic Acid (PCA), which is an antioxidant, anti-inflammatory and anti-apoptotic agent, on ovarian tissue and ovarian reserve against ischemia-reperfusion (IR) injury in a rat ovarian torsion model.BACKGROUND: Reactive oxygen radicals cause histopathological changes in the ovarian tissue during the reperfusion phase. PCA may have protective effects on ovarian tissue and reserve due to its antioxidant and antiapoptotic properties.METHODS: A total of 24 Wistar adult female rats were divided into 3 groups as the control (sham operation, n = 8), IR (Ischemia-Reperfusion, n = 8), and IR+PCA (Ischemia-Reperfusion + 80 mg/kg protocatechuic acid, n = 8). The IR and IR + PCA groups underwent 3 hours of ischemia followed by 3 hours of ovarian reperfusion. Protocatechuic acid (80 mg/kg) was administered to the IR+PCA group 30 minutes before reperfusion. After reperfusion, the ovaries were removed for histopathological and biochemical examination.RESULTS: Histopathological score and TUNEL+ cell count were significantly lower and AMH expression level was significantly higher in the IR+PCA group when compared to the IR group (p <0.05). However, in the comparison of the follicle counts, there was no statistically signifi cant difference between all groups. Due to the increase in antioxidant activity, the MDA levels were found to be significantly lower in the IR+PCA group compared to the IR group (p < 0.05).CONCLUSION: Protocatechuic acid may be an effective antioxidant in protecting ovarian tissue and follicle reserve against IR injury of the ovary (Tab. 1, Fig. 4, Ref. 36). T ext in PDF www.elis.skÖğe Effects of isotretinoin on new bone formation after maxillary sutural expansion(Urban & Vogel, 2021) Bulut, Musa; Korkmaz, Yasemin Nur; Erimşah, SevilayCorrection to: J Orofac Orthop (2020) 81:440–446 https://doi.org/10.1007/s00056-019-00209-2 The original version of this article unfortunately contained a mistake. The third bullet under the section Materials and Methods should read “Soybean oil (SOY) group: Soybean oil was administered via oral gavage (2 ml/kg) during the nursery period before expansion (an interval of 40 days), and during the expansion (period of 5 days) + retention period (period of 12 days—a total of 57 days)” instead of “Soybean oil (SOY) group: Soybean oil was administered via oral gavage (0.2 ml/kg) during the nursery period before expansion (an interval of 40 days), and during the expansion (period of 5 days) + retention period (period of 12 days—a total of 57 days)”.Öğe Effects of isotretinoin on new bone formation after maxillary sutural expansion(Urban & Vogel, 2020) Bulut, Musa; Korkmaz, Yasemin Nur; Erimşah, SevilayPurpose The aim of this study was to examine the effects of isotretinoin on new bone formation after maxillary sutural expansion in rats. Materials and methods A total of 32 maleWistar rats were selected and randomly divided into four groups. The isotretinoin group was treated with 7.5mg/kg isotretinoin, and the soybean group was treated with 2ml/kg soybean oil for 57 days. The substances were applied via oral gavage. The expansion-only and the control groups were not treated with any substance. In the experiment groups, expansion springs were applied on day 41 of the experiment, and after day 5 of expansion, a 12-day retention period was established. At the end of the experiment, all the animals were sacrificed, and their maxillae were dissected for histological evaluation. The numbers of osteoclasts, osteoblasts and formation of new bone and capillaries were evaluated on slides centered around the suture. Results The statistical analysis showed significant differences between the groups for the number of osteoblasts and osteoclasts (p< 0.001). In the experiment groups, higher numbers of osteoblasts and osteoclasts were detected in comparison to the control group (p< 0.001), while there was no significant difference between the experiment groups. Capillary formation and new bone formation in the isotretinoin group were found to be on a higher level than in the other groups (p< 0.001). Conclusion Isotretinoin had no negative effects on bone formation following the expansion of the maxillary suture in rats.Öğe Evaluation of the effect of Carvacrol on retinal neovascularization in rats(2021) Kaymaz, Abdulgani; Erimşah, SevilayObjectives: To compare the effect of intraperitoneal administered Carvacrol with bevacizumab in anoxygen-induced retinopathy (OIR) model in rats.Materials and Methods: Twenty-eight newborn rats were included in the study and the OIR model wascreated with the 50/10% oxygen style. The study consisted of 4 groups and each rat in the groups receivedan intraperitoneal injection of 0.01 mL once on the postnatal 14th day. While the OIR model was not createdin Group I (control group), it was created for Groups II, III, and IV. Groups I and II were injected with 0.9%NaCl solution, Group III with bevacizumab, and Group IV with carvacrol. The rats were sacrificed on thepostnatal 18th day.Results: Histopathological and immunohistochemical studies showed that the number of retinal vascularendothelial cells (RVECs) and nuclear factor (NF)-?B levels decreased similarly in Group III and Group IVcompared to Group II. RVECs values for Group I, Group II, Group III and Group IV were measured as1.26±0.80, 27.10±3.63, 7.54±1.38, and 6.22±1.22, respectively and it differed significantly between groups(p<0.001). Likewise, NF-?B levels were recorded as 0.61 ± 0.30, 4.36±0.65, 2.68±0.44, and 2.85±0.58,respectively and it differed significantly between groups (p<0.001). On the other hand, RVECs and NF-?Blevels were similar between Group III, and Group IV (p values were 0.58 and 0.91, respectively).Conclusions: The study demonstrated that carvacrol significantly reduced retinal pathologicalneovascularizations, RVECs, and NF-?B levels. Moreover, the observed effects were comparable to those ofbevacizumab.Öğe High quality human sperm selection for IVF: A study on sperm chromatin condensation(Elsevier Gmbh, 2019) Şaylan, Aslıhan; Erimşah, SevilayThe study consisted of semen samples of 20 male individuals who applied to Abant Izzet Baysal University Faculty of Medicine and participated in a spermiogram. The aim of this study was to determine how to obtain the healthiest spermatozoa by employing a variety of swim-up methods over differing time periods and without the use of centrifuge. Ejaculate samples were taken from the 20 patients and each patient's homogenized semen sample was divided into 4 groups without centrifugation. Group 1 was taken as the sample of untreated semen. For the other 3 groups, 250 mu l of medium was added in the semen samples. Afterwards, the samples were kept at 37 degrees C for different time periods, 30 min for Group 2, 60 min for Group 3 and 90 min for Group 4 in order for the spermatozoa to swim to the media in the upper layer. At the end of the periods, 10 mu l of propagation preparations were prepared from the swim-up fluid. Using Aniline Blue for chromatin condensation analysis, two hundred cells were immunostained by Caspase 3 for apoptotic analysis. Subsequently, the result of the four groups were compared for each test. The spermatozoa obtained at the end of the 30 min. of swim-up was compared to the spermatozoa obtained from the swim-up of 60 min., the swim-up of 90 min. It was found that the control group had statistically significant lower rates of apoptosis and was healthier in terms of chromatin integrity. The swim-up method without centrifugation is the best suited sperm preparation, based on sperm DNA integrity and sperm chromatin condensation.Öğe An Ideal Sperm Selection Method for the Intrauterine Insemination Treatment of Normozoospermic Infertile Patients(2020) Erimşah, Sevilay; Gonca, Süheyla; Kükner, Aysel; Duran, Bülent; Yazır, YusufhanAim: In this study, we evaluated the DNA integrity of sperms selected by using the conventional swim-up and density gradient centrifugation techniques and the new nanotechnology-based microfluidic chip method in order to determine the ideal sperm selection method for the intrauterine insemination treatment of normozoospermic infertile patients (NIPs). Materials and Methods: Semen samples obtained from 20 patients were divided into four equal fractions. Control, density gradient centrifugation, swim-up, and microfluidic chip (MC) groups were created, and the untreated (control) and treated (other) sperm samples from the four groups were examined for DNA integrity. Acidic aniline blue staining and the TUNEL method were used respectively for evaluating sperm chromatin condensation defects and DNA fragmentation. Dichlorofluorescein diacetate and flow cytometry were used to determine the reactive oxygen species levels. Results: We measured significantly lower values of chromatin condensation defects, DNA fragmentation and reactive oxygen species for the sperms selected with the MC method, compared to the sperms selected with the conventional methods (p<0.0001). Discussion and Conclusion: We found that the new MC method was more effective in selecting sperms with high DNA integrity, compared to the conventional methods. Accordingly, the MC method can be an ideal sperm selection method for use in the intrauterine insemination treatment of NIPs with high DNA fragmentation, apoptosis, and reactive oxygen species levels.Öğe Investigating the effects of carvacrol in rats using oxygen-induced retinopathy model(Wolters Kluwer Medknow Publications, 2021) Kaymaz, Abdulgani; Ulaş, Fatih; Çetinkaya, Ayhan; Erimşah, SevilayPurpose: Investigating the effects of intraperitoneal carvacrol administration in rats using the oxygen-induced retinopathy (OIR) model. Methods: A total of 28 newborn Sprague Dawley rats were used and the OIR model was created using the 50/10% oxygen model. The study composed of four groups in total. While the OIR model was not used in Group I (control group), it was created for Groups II, III, and IV. About 0.01 mL carvacrol, bevacizumab, or 0.9% NaCl was administered intraperitoneal (IP) to the rats in all groups on postnatal day (PND) 14 as follows: Group I and Group II were administered 0.9% NaCl, Group III was administered bevacizumab, and Group IV was administered carvacrol. On PND 18, rats were sacrificed and their right eyes were enucleated. Results: Histopathological and immunohistochemical studies showed that the number of vascular endothelial cells (VECs), vascular endothelial growth factor (VEGF), and tumor necrosis factor-alpha (TNF-alpha) decreased similarly in Group III and Group IV compared with Group II. VECs values for Group I, Group II, Group III, and Group IV were measured as 0 +/- 0, 26.45 +/- 4.57, 7.75 +/- 1.98, and 5.78 +/- 1.72, respectively, and it differed significantly between groups (P < 0.001). Likewise, VEGF levels were observed as 0.06 +/- 0.01, 3.31 +/- 0.53, 2.47 +/- 0.44, and 2.49 +/- 0.52, respectively, and it differed significantly between groups (P < 0.001). TNF-alpha levels were recorded as 0.06 +/- 0.01, 3.58 +/- 0.38, 2.46 +/- 0.49, and 2.29 +/- 0.25, respectively, and it differed significantly between groups (P < 0.001). VECs, VEGF, and TNF-alpha were similar between Group III and IV (range of P values were 0.486-0.998). Conclusion: The study demonstrated that carvacrol significantly reduced retinal pathological angiogenesis, NV, VEC nuclei count, VEGF, and TNF-alpha levels. Moreover, the observed effects were comparable to those of bevacizumab.Öğe Investigation of the effect of quercetin in an experimental oxygen-induced retinopathy model(2021) Kaymaz, Abdulgani; Ulaş, Fatih; Erimşah, Sevilay; Öztabag, Cansu KaraAim: To investigate of the effect of intraperitoneal (IP) quercetin and bevacizumab on oxygen-inducedretinopathy (OIR) model in rats.Methods: In the study, 28 newborn rats were used. The OIR model was performed with the 50/10% oxygentechnique. The study consisted of four groups as a control group (Group I) and OIR groups (Group II, III, andIV). IP injection applied to all groups on the postnatal day (PND) 14. Groups I and II were performed 0.9%NaCl, Group III was performed IP bevacizumab, and Group IV was performed IP quercetin. All animals weresacrificed on PND 18.Results: Based on the data obtained from immunohistochemical and histopathological examinations, thenumber of vascular endothelial cell (VEC), vascular endothelial growth factor (VEGF), and tumor necrosisfactor-? (TNF-?) levels were significantly reduced in Group III and IV compared to Group II. VECs levelswere 0±0, 32.69±5.77, 2.92±0.63, and 3.64±0.36 in Group I, Group II, Group III, and Group IV, respectively(p<0.001). Likewise, VEGF values were 0.15±0.01, 7.57±1.80, 2.45±0.45, and 2.46±0.49, respectively(p<0.001). As well as TNF-? values were 0.06±0.01, 8.22±2.24, 2.32±0.32, and 2.29±0.26 in Group I, GroupII, Group III, and Group IV, respectively (p<0.001). There was no significant difference between Group IIIand Group IV in terms of VEC, VEGF and TNF-? values (range of p values was 0.96-1.00).Conclusion: The results of the present study showed that quercetin administration significantly reduced theVEC number and suppressed VEGF and TNF-?. Quercetin's anti-inflammatory and anti-angiogenesis effectwas found to be similar to bevacizumab.Öğe Major ozonated autohemotherapy preconditioning ameliorates kidney ischemia-reperfusion injury(Springer/Plenum Publishers, 2016) Sancak, Eyüp Burak; Türkön, Hakan; Çukur, Selma; Erimşah, Sevilay; Akbaş, AlpaslanMedical ozone has therapeutic properties as an antimicrobial, anti-inflammatory, modulator of antioxidant defense system. Major ozonated autohemotherapy (MOA) is a new therapeutic approach that is widely used in the treatment of many diseases. The objective of the present study was to determine whether preischemic application of MOA would attenuate renal ischemia-reperfusion injury (IRI) in rabbits. Twenty-four male New Zealand white rabbits were divided into four groups, each including six animals: (1) Sham-operated group, (2) Ozone group (the MOA group without IRI), (3) IR group (60 min ischemia followed by 24 h reperfusion), and (4) IR + MOA group (MOA group). The effects of MOA were examined by use of hematologic and biochemical parameters consisting of neutrophil to lymphocyte ratio (NLR), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), ischemia-modified albumin (IMA), total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI). In addition, the histopathological changes including the tubular brush border loss (TBBL), tubular cast (TC), tubular necrosis (TN), intertubular hemorrhage and congestion (IHC), dilatation of bowman space (DBS), and interstitial inflammatory cells infiltration (IECI) were evaluated. In the IR group, compared to the Sham group, biochemical parameters indicating oxidative stress, NLR, IL-6, TNF-alpha, IMA, TOS, and OSI have increased. MOA reduced inflammation and oxidative stress parameters. Although TAS values have decreased in the IR group and increased in the MOA-pretreated group, no significant changes in TAS values were detected between the IR and MOA groups. The total score was obtained by summing all the scores from morphological kidney damage markers. The total score has increased with IR damage when compared with the Sham group (13.83 +/- 4.30 vs 1.51 +/- 1.71; p = 0.002). But, the total score has decreased significantly after application of MOA (5.01 +/- 1.49; p = 0.002; compared with the IR group). MOA preconditioning is effective in reducing tissue damage induced in kidney ischemia-reperfusion injury. The protective effect of MOA is mediated via reducing inflammatory response and regulating of reactive oxygen species (ROS). Renal histology also showed convincing evidence regarding MOA's protective nature against kidney injury induced renal ischemia-reperfusion. Consequently, MOA might be helpful in protecting the kidneys from IR-induced damage in humans, probably through the anti-inflammatory effect and reducing the total oxidant status.Öğe Metaphors of medical students about embryology education(Sakarya University, 2015) Erimşah, Sevilay; Terzi, Hakan Elçin; Fırat, Tülin; Kükner, Aysel; Aytekin, Ayşegül; İlter, Gizem; Söyler, Gizem; Özdemir, Havva İmran; Erimşah, SevilayMetaphor can be defined as characterization of a phenomenon in familiar terms. Metaphor researches were used in training studies especially. In our study we aim to get information about the embryology phenomenon of medical students, and to determine the influencing factors of this phenomenon with their reasons. Our study was composed of participation of 100 students from Abant Izzet Baysal University Faculty of Medicine Class II grader in the 2014- 2015 academic year. The result of evaluating the questionnaires 100 (30 male, 70 female) students’ metaphors were taken into account. The data were evaluated according to age, gender, the place where they stay during the educational life and the reason for selecting the faculty of medicine. In order to detect metaphors the forms were given to the students as “Embryology is similar/like ................…, because ……………” and requested to complete the sentence with the reasons. The number and percentage of produced metaphors were evaluated. The results of the questionnaire show that there are 75 metaphors used by the students and the metaphors are classified into 8 different categories after the evaluation. According to the findings of the study, the mostly produced metaphors were life, seed, miracle, etc. Consequently, metaphors can be used as a powerful research tool in the understanding and explanation of personal perceptions of embryology education among the students of medicine faculty. Therefore, metaphors help us to develop of educational methods and devices for embryology lessons. © The Turkish Online Journal of Educational Technology.Öğe Protective effects of N-acetyl cysteine on undescended testis after orchiopexy: A rat-model study(Coll Physicians & Surgeons Pakistan, 2023) Kemahlı, Eray; Üyetürk, Uğur; Çetinkaya, Ayhan; Erimşah, Sevilay; Üyetürk, Ümmügül; Gücük, AdnanObjective: To assess the effectiveness of utilising N-acetyl cysteine (NAC) to treat tissue damage brought on by undescended testis (UT) in rats after orchiopexy.Study Design: Experimental study.Place and Duration of the Study: Bolu Abant Izzet Baysal University, Bolu, Turkey, from January 2018 to June 2020.Methodology: The UT model was created by administering flutamide to pregnant rats. Four groups of animals were created as the control group (offsprings of pregnant rats without flutamide), group II (UT), group III (UT + orchiopexy), and group IV (UT + orchiopexy + NAC); each containing eight animals.Results: Group IV had a higher level of glutathione peroxidase than groups III and II (p=0.001 and p=0.002, respectively). Malondialde-hyde was reduced in group IV compared with groups III and II (both p<0.001). There were differences in mean apoptotic cell counts (ACC) among the groups (p<0.001). ACC in group IV was lower than in group III (p<0.001). Sperm counts were higher in group IV than in groups III and II, and in group III they were higher than group II (p<0.001 all) and similar between groups IV and control group (p=0.102).Conclusion: Orchiopexy reduced UT-related testicular damage, additionally using NAC following orchiopexy may further reduce testic-ular damage through its antioxidant effects.Öğe Sesamol ameliorates acute kidney injury resulting from complete unilateral ureteral obstruction in the rats(Lahore Medical Research Center LLP, 2021) Üyetürk, Uğur; Çetinkaya, Ayhan; Bostancı, Emre; Erimşah, Sevilay; Öztürk, HayrettinObjectives: We aimed to evaluate the effectiveness of sesamol in preventing the progressive renal damage caused by complete unilateral ureter obstruction (CUUO). Methods: Twenty four Wistar rats were separated into three groups: In the group 1 rats (the sham-control, n = 8), the ureters were exposed and manipulated, but not ligated. In the group 2 rats (CUUO-untreated, n=8), CUOO was accomplished by double-ligating the left ureter using 5-0 silk ties. In the group 3 rats (CUUO -Sesamol treated, n=8), CUOO was created as in the Group 2 rats, also given sesamol daily, commencing on the day of CUUO surgery. The rats' left kidneys were examined histologically and biochemically within the 8th day Results: Histopathological total score, apoptotic index and MDA values in the group 2 show a significant increase when compared to the group 1. These values were significantly decreased in the group 3 rats when compared to the group 2 rats. Tissue GPx value in the group 2 show a significant decrease when compared to Sham-control group. However, these values were significantly increased in the group 3 rats when compared to the rats 2. Polymorph nuclear leukocyte infiltration in peritubular areas was observed in group II, with moderate interstitial fibrosis and tubular dilatation. Despite the presence of dilated tubule structures in group III, this group showed less bleeding, leukocyte infiltration, and fibrosis than group II. Conclusion: The results of this study have shown that CUOO-induced renal damage can be reduced using sesamol, possibly due to its antioxidant properties. The mechanism by which sesamol causes these effects merits further investigation.
