Yazar "Bozat, Bihter Gökçe" seçeneğine göre listele
Listeleniyor 1 - 16 / 16
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Anxiolytic, antioxidant, and neuroprotective effects of goji berry polysaccharides in ovariectomized rats: experimental evidence from behavioral, biochemical, and immunohistochemical analyses(Tubitak Scientific & Technical Research Council Turkey, 2020) Pehlivan Karakaş, Fatma; Coşkun, Hamit; Soytürk, Hayriye; Bozat, Bihter GökçeRecent studies have indicated that polysaccharides, the main component of the Lycium barbarum L. fruit, have beneficial effects (e.g., anxiolytic, antioxidant, and neuroprotective) on humans and rodents. However, the effects of different dosages of such polysaccharides on ovariectomized rats and their underlying mechanisms in the brain have not been evaluated in the literature. Here, we aimed to evaluate the effects of the high and low doses of polysaccharides obtained from Lycium barbarum fruits (HD-LBP and LD-LBP, respectively) on anxious behaviors via behavioral (using the OFT and EPM), biochemical (using ELISA), and immunohistochemical (using immunohistochemical staining) measures in detail. Two weeks after ovariectomy, the rats were randomly assigned to either the treatment conditions [control (DW, 3 mL/kg, p.o., per day), LD-LBP (20 mg/kg, 3 mL/kg, p.o., per day), HD-LBP (200 mg/kg, 3 mL/kg, p.o., per day), 17 beta-ES (1 mg/kg, 3 mL/kg, p.o., per day), DZ(1 mg/kg, 3 mL/kg, p.o., per day)] or operation type [SHAM (pseudo-ovariectomized) and OVX (ovariectomized)]. The treatments were applied for 30 consecutive days, and then serum and brain tissue samples of all rats were collected. Biochemical (SOD, CAT, GPX, MDA, and 17 beta-ES) and immunohistochemical (BDNF, SER, and apoptosis) analyses of the samples were performed as well. The rats administered HD-LBP and LD-LBP were less anxious than the control groups. The HD-LBP-treated rats had high levels of SOD and low levels of MDA in their serum samples. Moreover, HD-LBP and drug-treated groups had a high number of SER receptors and BDNF-positive cells and a low number of TUNEL-positive cells in their hippocampal brain tissues. The HD-LBP treatments decrease anxious behavior by increasing antioxidant enzyme activities, hippocampal SER and BDNF neurotransmitter levels and decreasing the TUNEL-positive cell count of ovariectomized rats. Given these findings, we suggest that menopause-induced symptoms of anxiety can be reduced by polysaccharides obtained from goji berry fruits, and that these findings will be beneficial for the production studies of natural herbal-origin antianxiety (anxiolytic) drugs in the future.Öğe Can hyperoxic stress cause susceptibility to acute seizure in the neonatal period?: A rat study(Taylor & Francis Ltd, 2022) Dilek, Mustafa; Soytürk, Hayriye; Bozat, Bihter Gökçe; Hancı, Fatma; Taş, Sinan; Kabakuş, NimetObjective: Preterm neonates encounter hyperoxia relatively early, and are more exposed to hyperoxic stress due to their insufficient antioxidant defense mechanisms. This study was planned around the hypothesis that this hyperoxic effect may cause a disposition to future acute seizures. Methods: This study was composed of two main groups Hyperoxy and Control (Room air with normal O-2 levels) Groups. Group 1 - hyperoxia (Study): The experimental group consisted of premature newborn rats exposed to hyperoxia with their dams from birth to postnatal day 5. Group 2 - room air (Control): The group was not exposed to hyperoxia and housed the same room air and temperature as their dams. Female, Acute Epilepsy Female, Male, Acute Epilepsy Male, and a total of eight subgroups were formed in both the control and hyperoxia groups. When the rats were two months old, intracranial electrodes were attached to obtain electrocorticography (ECoG) recordings. Pre-model recordings were taken, after which an acute pentylenetetrazole (PTZ) model of absence seizure was induced by the intraperitoneal administration of PTZ at 50 mg/kg. ECoG records were examined using the PowerLab system for 180 min. Spike wave number and duration, Spike wave frequency and amplitude data were evaluated.Results: Seven female and three male rats were exposed to hyperoxia, and a control group of five female and three male rats were included in the study. The median interquartile range for spike wave latency in the hyperoxia and control groups were 1112 (644-1545) and 654 (408-1152), frequency 4476 (3120-7421) and 3934 (2264-4704), and amplitude data 0.68 (0.59-0.79) and 0.52 (0.37-0.67), respectively. Although a difference was observed in median values capable of constituting susceptibility to epilepsy, the difference was not statistically significant (p > 0.05). In terms of gender, spike-wave counts were significantly higher in female rats (p < 0.05). Females exposed to hyperoxia were more susceptible to epilepsy than both males and females in the control group (p < 0.05).Conclusion: Exposure to hyperoxia in the first days of life of premature neonates due to their susceptibility to oxidative stress and insufficient antioxidant mechanisms, can cause a disposition to acute seizures. As a result, females exposed to hyperoxia during the neonatal period may be prone to epilepsy in maturity.Öğe Diversity exists in development parameters and enhancement of antioxidant mechanisms of some einkorn and bread wheats under combined water deficits and salt stress(Mattioli 1885, 2017) Karakaş, Fatma Pehlivan; Bozat, Bihter Gökçe; Aslan, Didem; Zencirci, NusretIntroduction. Climatic changes worsen the production of wheat, an important stable crop while improving its some nutritional quality characteristics. Methods. Therefore, the purpose here was to evaluate some yield limiting factors as well as some quality characteristics in 8 bread wheat (Triticum aestivum L.) cultivars (Kirac - 66, Kenanbey, Flamura - 85, Momtchill, Bayraktar - 2000, Tosunbey, Pandas, and Pehlivan) and 8 einkorn (Triticum monococcum spp. monococcum) populations (Populations - 4, 5, 6, 9, 10, 11, 14, and 15) under three different osmotic pressures (0.0 MPa., -0.5 MPa., or -1.0 MPa.) and three different salt concentrations [0.0 (distilled water), 50 mM or 100 mM NaCl]. Moreover, total phenolic content (TPC) and total flavonoid content (TFC), and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activities were determined. Results. Among bread wheat cultivars, Pandas had the longest shoot length, root length, the heaviest fresh shoot weight, dry shoot weight, and fresh root weight in the control group. In the einkorn populations, Pop - 6 had the longest shoot length, root length, the heaviest fresh shoot weight, dry shoot weight, and fresh root weight again in the control group. The heaviest dry root weight was obtained in Momtchill and Pop - 9 under drought stresses. The TPC was the highest in Kirac - 66 and in Pop - 10 under salt stress and the TFC was in Pehlivan under salt stress. The methanol extracts of Kirac - 66, Pop - 9, and Pop - 15 obtained under salt stress had stronger free radical scavenging activity than by ascorbic acid. Conclusion: Kirac - 66 had the highest free radical scavenging antioxidant activity under salt stress and higher secondary metabolite products (as flavonoids), which indicated the highest tolerance system under both drought and salt stress. Kirac - 66, which was improved for poorer, drier, and cooler lands had better root and metabolite production under combined drought and salt stress as expected.Öğe The effect of intra-amygdalar leptin administration on anxiety, depression and learning behaviors in rats(Ondokuz Mayis Universitesi, 2021) Soytürk, Hayriye; Bozat, Bihter Gökçe; Coşkun, Hamit; Karakaş, Fatma PehlivanLeptin is released by adipose tissue. Leptin can cross the blood-brain barrier and bind to receptors on neurons in brain areas to exert its biological function when released into circulation. This study aimed to determine the influences of intra-amygdalar administration of high and low doses of leptin on anxiety, depression, learning behaviors of rats. In the experimental protocol I, intra-amygdalar injection of high and low doses of leptin (0.1 and 1?g/ kg) and saline were administered 30 min before the behavioral tests. Then, the animals were exposed to open field, elevated plus maze, Porsolt and Morris water maze tests for measuring of behaviors. In experimental protocol 2, the cerebrospinal fluids of all groups of experimental protocol 1 were collected by microdialysis method and then were analyzed by HPLC. The effect of the low dose of leptin was significant on the open field. The effect of the high and low dose of leptin was significant on the elevated plus maze test. The effect of the low dose of leptin was significant on mobility in the center of the Porsolt. A high dose of leptin group had spent less time around the platform than controls in the Morris water maze test. HPLC analysis showed that the amount of serotonin and glutamate in the amygdala region increased after low dose leptin administration. Intra-amygdalar injection of low doses of leptin may decrease anxiety and depression-like behavior in rats by increasing serotonin and glutamate levels in the amygdala. © 2021 Ondokuz Mayis Universitesi. All rights reserved.Öğe The Effect of Wet Cupping Therapy on Anxiety and Thiol/Disulfide Homeostasis in Premature Ovarian Failure: An Experimental Study(2022) Çelik, Humeyra; Çetinkaya, Ayhan; Bozat, Bihter Gökçe; Düzcü, Selma Erdoğan; Alişik, Murat; Yis, Özgür MehmetObjective: The effect of wet cupping therapy on anxiety\rand thiol/disulfide homeostasis was evaluated in rats with premature\rovarian failure (POF) induced by cyclophosphamide (CYC). Material\rand Methods: Twenty four Wistar Albino female rats were divided\rinto control (n=6), POF (n=6), wet cupping (n=6), POF+wet cupping\r(n=6) groups. CYC at a dose of 200 mg/kg was injected intraperitoneally\rto POF and POF+wet cupping groups. One week later, wet cupping\rtherapy was applied to wet cupping and POF+wet cupping groups for\r5 minutes. The next day, Open Field Test and Elevated Plus Maze Test\rwere applied to all experimental groups to evaluate anxiety. After sacrification,\rto assess pattern formation, endometrial thickness and ovarian\rreserve were measured with hematoxylin-eosin staining.\rThiol/disulfide homeostasis and erythrocyte oxidized-reduced glutathione\rlevels were measured in serum to measure oxidative stress. Results:\rAlthough there was no statistical significance, it was observed\rthat anxiety decreased in POF+wet cupping compared to POF group.\rThere was no change in thiol-disulfide values in POF+wet cupping\rcompared to POF group (p>0.05). Conclusion: Wet cupping therapy\rdid not reduce anxiety in the experimental POF model and did not shift\rthe balance to positive in thiol/disulfide homeostasis in favor of thiols.\rHowever, the obtained graphics, numerical data and high standard deviation\rsuggest that meaningful data could be obtained with more subjects.Öğe The effects of leptin administration to amygdala region on anxiety, depression and learning behaviors: change in seretonine and glutamate levels(Wiley-Blackwell, 2016) Orallar, Hayriye; Karakaş, Fatma Pehlivan; Bozat, Bihter Gökçe; Coşkun, HamitAIM: Leptin is a hormone secreted by adipose tissue and involved in modulation of food intake. In addition to these functions, it plays a role in various physiological events such as anxiety, depression and learning. The aim of this study was the investigation of effects of leptin administration to amygdala on anxiety, depression and learning behaviors. In addition, changing of serotonin and glutamate neurotransmitter levels interaction between behavior. METHODS: Presentstudy used Wistar albino rats. Animals were separated 3 groupssuch aslow dose (0.1µg/kg) and high dose (1µg/kg) leptin and saline (control) intraamigdalar administration group. In thisstudy, we planned two procedures. Firstly, a special cannula was implanted into amygdala region of rats and leptin and saline were injected in thisregion by infusion pump. After leptin injections, behavioral tests were performed. In the second study, the extracellular fluid was collected by microdialysis from the amygdale region.The levels of serotonin and glutamate were measured by HPLC. RESULTS: The first experiment demonstrated that in the open field, low dose leptin administrated rats spent more time at the center (F(2,57)= 3.97 p=0.02), showed more zone transition than control ones (F(2,57)= 3.22 p=0.04). In the elevated plus maze, high and low doses leptin administrated rats spent more time in the open arms(F(2,55)= 6.77 p=0.002) and also high dose leptin injected rats showed more frequency of entering open arms (F(2,55)= 9.97 p=0.001). In Porsolt test, low dose leptin administrated ratsshowed high mobility in center than sham group (F(2,55)= 4.30 p=0.02). In the Morris water maze, low dose leptin administrated rats spent more time over the platform (F(2,45)= 3.50 p=0.04). The second experiment demonstrated that serotonin and glutamate levels increased in the first hour after the leptin injections (p<0.05). CONCLUSIONS: Present study suggests that both high and low leptin administration to rat brain playsa role as anxiolytic and antidepression agents in ratsÖğe The effects of lycium barbarum l. polysaccharides on learning behaviors of young ovariectomized female rats(Wiley-Blackwell, 2016) Bozat, Bihter Gökçe; Karakaş, Fatma Pehlivan; Orallar, Hayriye; Coşkun, HamitAIM: Lycium barbarum Linnaeus is also known as goji berry, wolfberry or super fruit. The red-orange and sweet fruits of goji berryhave been used in herbal medicine and health food for the thousand years. L. barbarum fruits have polysaccharides which have many beneficial effects for human such as ocular neuroprotective, antioxidant, immunomodulator, hepatic pretection and antitumor effects. In the present study, the aim was to investigate the effects of the L. barbarum polysaccharides (LBP) on learning behavior in ovariectomized young female rats(two months age) using the Morris water maze test. METHODS: Two weeks after ovariectomy applications, rats were divided into five groups: control (distile water 3 mL/kg, gavage, per day), low dose LBP (20 mg/kg, 3 mL/kg, gavage, per day), high dose LBP (200 mg/kg, 3 mL/kg, gavage, per day), estrogen (1 mg/kg, 3 mL/kg, gavage, per day) and donepezil (1 mg/kg, 3 mL/kg, gavage, per day) and two subgroup within the each group: sham (pseudo ovariectomized rat) and overiectomized groups. After all treatments were applied for thirty consecutive days, behavioral test was applied. Blood serum samples of all rats were collected and levels of antioxidant enzymes (SOD, CAT, GPX, MDA and E2) of the samples were detected by ELISA method. All animals weights were measured weekly. RESULTS: The findings of the present experiment demonstrated that platform finding time and travelled distance of the LBP administred ovariectomized groups were less than estrogen and donepezil administred groups. CONCLUSIONS: In conclusion, LBP enhances the learning performance and antioxidant enzyme activity of ovariectomized female rats.Öğe The effects of the lycium barbarum l. polysaccharides on the anxiety, depression and learning behaviors of the ovariectomized rats(Bolu Abant İzzet Baysal Üniversitesi, 2017) Bozat, Bihter Gökçe; Karakaş, Fatma PehlivanLycium barbarum L. polisakkaritlerinin (LBP) overektomili dişi sıçanlarda anksiyete, depresyon ve öğrenme davranışları üzerine olan etkilerini incelemek için, Açık Alan, Yükseltilmiş artı labirent, Porsolt ve Morris su tankı testleri kullanılmıştır. Overektomi operasyonundan iki hafta sonra, sıçanlar 7 ana gruba (tedavi): kontrol [distile su, 3 mL/kg, oral gavaj (o.g.), günlük], düşük doz LBP (20 mg/kg, 3 mL/kg, o.g., günlük), yüksek doz LBP (200 mg/kg, 3 mL/kg, o.g., günlük), 17 beta estradiol (1 mg/kg, 3 mL/kg, o.g., günlük), diazepam (1 mg/kg, 3 mL/kg, o.g., günlük), imipramine (2,5 mg/kg, 3 mL/kg, o.g., günlük), donepezil (1 mg/kg, 3 mL/kg, o.g., günlük) ve her grup kendi içinde iki alt gruba (operasyon): sham (yalancı overektomili sıçan) ve overektomili (ovx) sıçan gruplarına ayrılmışlardır. Tedaviler 30 gün uygulandıktan sonra tüm sıçanların serum ve beyin doku örnekleri toplanmıştır. Biyokimyasal [superoksit dismutaz (SOD), katalaz (CAT), glutatyon peroksidaz (GPX), malondialdehit (MDA) ve 17-? estradiyol (17-? ES)] ve immunohistokimyasal [beyin türevli nötrofik faktör (BDNF), serotonin reseptör (SER) and apoptoz] analizleri yapılmıştır. Davranış çalışmasının bulguları, yüksek ve düşük doz LBP uygulanan sıçanların daha az kaygılı ve daha az depresif olduğunu ve öğrenme performansının yüksek olduğunu göstermektedir. Biyokimyasal çalışma bulgularında, yüksek doz LBP uygulanan sıçanların serumları yüksek SOD ve düşük MDA seviyesi göstermektedir. Immunohistokimyasal çalışma bulguları, yüksek doz LBP ve ilaç tedavilerinin sıçanların yüksek seviye SER ve BDNF pozitif hücre ve düşük seviyede TUNEL pozitif hücreye sahip olduğunu göstermiştir. Sonuç olarak, yüksek doz LBP tedavisi, serum antioksidan enzim aktivitesini ve hipokampal SER ve BDNF seviyelerini artırarak, ayrıca TUNEL pozitif hücre sayısını azaltarak overektomili sıçanların anksiyete, depresyon gibi davranışlarını önemli ölçüde azaltmış ve öğrenme performanslarını arttırmıştır.Öğe Evaluation of the protective effect of the cup therapy on the epileptic seizure in rats(Düzce Univ, Fac Medicine, 2021) Çetinkaya, Ayhan; Karamaden, Esra Fidan; Göksu, Selim; Bozat, Bihter Gökçe; Demir, ŞerifObjective: Cup therapy has an important place in traditional and complementary medicine applications. The purpose of our study, this is the first time to investigate the protective effect of cup therapy in rats on experimentally generated epileptic seizures in new rat modeling created by different anatomic regions. Methods: A total of 42 Wistar albino rats were randomly divided into 6 groups (n:7). The "new dry cup" was applied to the G1 group, and the "new wet cup" model was applied to the G2. In the G3 group, an "epilepsy model" (PTZ, 35 mg / kg) was created and "diazepam" (2.5 mg / kg) was given to G4. "Dry cup" and "wet cup" models were applied to the G5 and G6 groups, respectively. 24 hours after the cupping therapies, the rats were injected with PTZ and the epilepsy behavior scores of the rats in all groups were recorded for 20-30 minutes. Results: In the 'Open Area' and 'Elevated Plus Maze' tests, there was no behavioral difference between the cup therapy group and the control group (p > 0.05). Given all the parameters, the G4 group significantly reduces the seizure compared to other groups (p < 0.05). There is a significant difference in G2, G5 and G6 groups compared to G3 in the phases parameter (p < 0.05). Conclusions: In this study, the new wet cup therapy (G2), which was applied for the first time, had a protective effect on seizures. G2, G5 and G6 groups are observed to suppress seizures compared to G3. Our findings are expected to contribute greatly to animal model analysis in the future.Öğe Fluctuation in secondary metabolite production and antioxidant defense enzymes in in vitro callus cultures of goat's rue (Galega officinalis) under different abiotic stress treatments(Springer, 2020) Karakaş, Fatma Pehlivan; Bozat, Bihter GökçeExogenous abiotic stress treatments are an effective way of accelerating stress tolerance by modulation of accumulation of various secondary metabolites in pharmaceutical, medicinal, and economically important plant species. This research aimed to evaluate the effects of nutrient, chemical, temperature, and ultraviolet (UV) radiation abiotic stress on fluctuations in selected secondary metabolites and the antioxidant defense system ofGalega officinalisL. (goat's rue) callus tissue cultures. Stress treatments were achieved by exposing callus cultures of goat's rue to five different strengths of Murashige and Skoog (MS) medium (2MS, MS, MS/2, MS/4, and MS/8), four different temperatures (4 degrees C, 22 degrees C, 35 degrees C, and 45 degrees C), two chemicals [mercury chloride (HgCl2) and copper sulfate (CuSO4)], and three types of UV radiation (UV-A, UV-B, and UV-C) for three different durations (15, 30, and 60 min). The amounts of selected phenolic secondary metabolites (apigenin,p-coumaric acid, genistein, kaempferol, luteolin, rutin hydrate,trans-ferulic acid, salicylic acid, chlorogenic acid, naringenin, and isorhamnetin) in callus cultures of goat's rue was determined by liquid chromatography-electrospray ionization-multistage/mass spectrometry (LC-ESI-MS/MS). The dominant molecule wasp-coumaric acid in all stress treatments. The highest amounts of luteolin, genistein,p-coumaric, naringenin, apigenin,trans-ferulic acid, salicylic acid, and rutin were determined in the MS/8, MS/8, MS/2, MS/2, MS/4, UV-A (15 min), UV-A (15 min), and 4 degrees C treatments, respectively. Interestingly, the percentage increase (379.01%) in the total selected phenolic molecules was highest in the MS/8 treatment. The superoxide dismutase and catalase antioxidant enzyme activity were highest in the CuSO(4)and MS/4 treatments, respectively. Moreover, the MS/4 treatment resulted in the highest proline content. The total phenolic content was highest after the temperature (4 degrees C) and UV radiation (UV-A 30 min) treatment. The UV-A (15 min) treatment had the strongest antioxidant activity, with the highest total flavonoid content and the lowest concentration inhibiting 50% of DPPH (IC50) value. These outcomes indicated that different abiotic stress factors caused extraordinary variations in antioxidant levels and secondary metabolite accumulation as a way of regulating the plant's defense system. The newly developed protocol in the current study can be used to provide the desired amount of special secondary metabolite production and establish a new valuable herbal medicine to cure many chronic diseases, as well as in the pharmaceutical area and other related areas. Key message Different abiotic stress factors caused extraordinary variations in the antioxidants levels and secondary metabolite accumulation as a way of regulating the plant's defense system.Öğe Investigation of electrocorticogram recordings in an experimental model of penicillin-induced epilepsy in female rats at different stages of the oestrus cycle(Wiley, 2022) Çetinkaya, Ayhan; Kara, Sinem; Bozat, Bihter Gökçe; Yabalak, AhmetMeeting AbstractÖğe Lycium Barbarum L. (Goji Berry) fruits improve anxiety, depression-like behaviors, and learning performance: The moderating role of sex(2016) Karakaş, Fatma Pehlivan; Coşkun, Hamit; Sağlam, Kadir; Bozat, Bihter GökçeThe aim of this research was to investigate the effects of the methanol extract obtained from Lycium barbarum fruits on anxiety, depression-like behaviors, and spatial memory in Wistar albino rats. A total of 28 rats were selected, randomly assigned to four experimental groups, and tested by means of the open field and elevated plus maze tests for anxiety-like behaviors, the forced swim test for depression-like behaviors, and the Morris water maze test for spatial memory. The findings demonstrated that in the open field, L. Barbarum-administered rats spent more time at the center and showed more mobility and velocity than controls. In the elevated plus maze, L. Barbarum-administered rats spent more time in the open arms, spent less time in the closed arms, and showed more mobility and velocity. In the Porsolt test, L. Barbarum-administered rats showed less immobility. In the Morris water maze, L. Barbarum-administered rats took more time to find the platform. However, females were better at finding the platform than males. The methanol extract of L. Barbarum fruits decreased anxiety and depression-like behaviors and interacted with sex on spatial memory.Öğe Multipl skleroz ve diğer demiyelinizan hastalıklarda herpes virüslerin nöronal otoantikorlar ile ilişkisinin araştırılması(Bolu Abant İzzet Baysal Üniversitesi, 2022) Bozat, Bihter Gökçe; Türkoğlu, Şule AydınDemiyelinizasyona yol açan otoimmün yanıtın tetiklenmesine neden olacak muhtemel mekanizma ve bu mekanizmanın virüslerle ilişkisi hakkında çok az şey bilinmektedir. Bu çalışmada, Multipl Skleroz (MS) ve Nöromiyelitis Optika Spektrum Bozukluğu (NMOSD) olgularında Anti-EBV, Anti-HHV6A pozitiflik seviyelerinin Anti-AQP4, Anti-flotillin, Nörofilament Hafif Zincir (NfL) ve Nogo-A seviyeleri ile olası ilişkilerinin tespit edilmesi amaçlanmaktadır. Etik Kurul onayı 04.02.2020/74 sayılı karar ile alındı. Kliniğimize başvuran 19-54 yaş arası MS (n=19), NMOSD (n=8) ve Psödotümör Serebri (PTS, n=15) olgularının tanı amaçlı alınan BOS örnekleri, ELISA ve IFA yöntemleri (BAİBÜ BAP 2021.08.32.1503) ile çalışıldı. Grup karşılaştırmalarında Bağımsız Örneklem T/Mann Whitney-U, Çapraz Tablolar-Fisher's Exact ve Pearson/Spearman korelasyon analiz testleri kullanıldı. p<0,05 değeri istatistiksel olarak anlamlı kabul edildi. Anti-AQP4 pozitiflik yüzdesi en fazla PTS grubundaydı, p=0,055. Anti-HHV6A pozitif MS (p=0,046) ve PTS (p =0,015) olgularının Nogo-A seviyeleri daha yüksekti. Anti-flotillin pozitif MS olgularında NfL seviyesi daha az gözlendi, p=0,051. MS (p=0,046) ve PTS (p=0,011) olgularının Nogo-A seviyeleri ile Anti-HHV6A pozitiflikleri, NMOSD olgularının hastalık başlangıç yaşları ile NfL seviyeleri (p=0,027) ve PTS olgularının Nogo-A ve NfL seviyeleri arasında pozitif yönde korelasyon saptandı, p=0,031. Elde edilen sonuçlara göre tanı aşamasında PTS olgularında Anti-AQP4 pozitifliklerinin değerlendirmesinin yapılması önerilmektedir. Anti-EBV pozitifliğinin, üç olgu grubunda yüksek olması, ayırıcı tanı yerine tedavi stratejilerinde kullanılacak bir parametre olabileceği düşünülmektedir. Anti-HHV6A'nın ayırıcı tanıda daha etkili olduğu ve özellikle aksonal hasarı gösteren yeni biyobelirteçlerle birlikte çalışılması önerilmektedir. Ayırıcı tanıda Anti-flotillin ile ilgili çok merkezli ve daha fazla olgu sayısı ile yapılacak yeni çalışmalara ihtiyaç duyulmaktadır.Öğe Neuroprotective effects of goji berry (Lycium barbarum L.) polysaccharides on depression-like behavior in ovariectomized rats: Behavioral and biochemical evidence(Medicinska Naklada, 2023) Soytürk, Hayriye; Bozat, Bihter Gökçe; Karakaş, Fatma Pehlivan; Coşkun, Hamit; Fırat, TülinAim To assess the protective effects of goji berry (Lycium barbarum L.) polysaccharides (LBP) on depression-like behavior in ovariectomized rats and to elucidate the mechanisms underlying these effects. Methods One hundred female Wistar albino rats (three months old) were randomly assigned either to ovariectomy (n = 50) or sham surgery (n = 50). After a 14-day recovery period, the groups were divided into five treatment subgroups (10 per group): high-dose LBP (200 mg/kg), low-dose LBP (20 mg/kg), imipramine (IMP, 2.5 mg/kg), 17 beta estradiol (E2, 1 mg/kg), and distilled water. Then, rats underwent a forced swimming test. We also determined the levels of serum antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase, and malondialdehyde), E2 levels, hippocampal brain-derived neurotrophic factor (BDNF), 5HT2A receptor, and transferase dUTP nick end labeling (TUNEL)-positive cells. Results Both low-dose LBP and imipramine decreased depression-like behavior by increasing serum superoxide dismutase activity and by decreasing serum malondialdehyde level. Furthermore, low-dose LPB, high-dose LBP, and imipramine increased the number of 5-HT2A receptor-and BDNF-positive cells but decreased the number of TUNELpositive cells in the hippocampus. Conclusion This is the first study to show the antidepressant effect of LBP. Although additional research is needed, LBP may be considered a potential new antidepressant.Öğe Phenolic content, antibacterial and antioxidant potential of several edible agaricomycetes mushrooms sold in public bazaar in Bolu, Turkey(Begell House Inc, 2023) Karakaş, Fatma Pehlivan; Türker, Arzu Uçar; Bozat, Bihter GökçeEdible mushrooms are important natural foods that have been used since ancient times due to their delicious taste, high nutritional value, and various biological activities. In this study, antibacterial (against 10 different human pathogens) and antioxidant potentials (free radical scavenging activity and total phenol-flavonoid content) of 13 different wild-growing and the most popular edible mushrooms (Lactarius salmonicolor, L. deliciosus, L. volemus, L. piperatus, Ramaria sp., Cantharellus cibarius, and Pleurotus ostreatus) sold in a public bazaar in Bolu-Turkey were evaluated. Strong antibacterial capacities were observed with all tested Lactarius species, as well as M. oreades, A. campestris, T. terreum, H. repandum, and Ramaria sp. Significant antibacterial potentials of T. terreum and C. comatus against Staphylococcus epidermidis, Serratia marcescens, and Proteus vulgaris were observed for the first time. H. repandum strongly inhibited all tested Gram-positive bacteria (Staphylococcus aureus, S. epidermidis, and Streptococcus pyogenes). It was remarkable to reveal strong growth inhibition of M. oreades against S. aureus (29.33 +/- 1.11 mm) for the first time. The highest total phenolic content was revealed with L. delicious (193.42 +/- 0.77 mg GAE/g dw and 198 +/- 0.79 mg TAE/g dw), while L. salmonicolor had the highest total flavonoid content (358.33 +/- 15.27 mg CE/g dw). B. edulis demonstrated the strongest antioxidant activity with the lowest IC50 value (4.48 +/- 0.04 mu g/mL). Moreover, L. deliciosus and M. oreades also had high antioxidant potential with the lowest IC50 values (4.76 +/- 0.01 mu g/mL and 4.77 +/- 0.02 mu g/mL, respectively). Total phenolic contents of tested mushrooms were highly correlated with their antioxidant capacities. L. delicious is the most well-known and the most delicious mushroom sold in Bolu's public bazaar. The obtained results for this mushroom were notable in terms of the greatest total phenol-flavonoid content and strong antioxidant capability. The antioxidant capacity and polyphenol content of L. deliciosus, M. oreades, H. repandum, A. campestris, and B. edulis, as well as their antibacterial activity, were quite outstanding, and our findings demonstrated the importance of these mushrooms as nutraceutical products.Öğe Protective effect of cup therapy model in rats on epileptic seizures by determination of different anatomic region(Wiley, 2019) Çetinkaya, Ayhan; Fidan, Esra; Göksu, Selim; Bozat, Bihter Gökçe; Demir, Şerif[No Abstract Available]
