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Öğe Accessory spleen presenting as mass in the left upper abdomen(College Physicians & Surgeons Pakistan, 2008) Yağmur, Yusuf; Öztürk, HayrettinAn enlarged accessory spleen, 10 x 8 x 6 cm in diameter, adjoining the spleen appearing as a mass on CT and ultrasonography is reported herein. Accessory spleen is an ectopic mass of healthy splenic tissue separate from the main body of the spleen. It should be considered the differential diagnosis of an enlarged mass in the left upper quadrant.Öğe Adrenocortical tumor: Report of two cases(2006) Öztürk, Hülya; Öztürk, Hayrettin; Dokucu, Ali Ihsan; Duran, HatunFunctioning adrenocortical tumor is rare in children. Although most are benign, both benign and malignant lesions may present with virilizing and Cushingoid features. We present two cases of adrenocortical tumor. The symptoms and signs at presentation were virilization. Cushingoid features, precocious puberty and hypertension. Both children underwent resection of the tumors. The presenting symptoms, postoperative course, adjuvant therapy, clinical course are described and the literature is reviewed.Öğe Beneficial effect of dexmedetomidine on testicular ischemia - reperfusion injury in rats(Wroclaw Medical University, 2008) Terzi, Hakan; Öztürk, Hülya; Buğdaycı, Güler; Öztürk, HayrettinBackground/Objectives. The present study was designed to determine the beneficial effect of dexmedetomidine (Dex) on torsion-detorsion-induced histopathological changes in experimental testicular ischemia/reperfusion (I-R) injury in rats. Material and Methods. Twenty-one male Sprague-Dawley rats were separated into three groups of seven rats each. A sham operation was performed in group 1 (control). In group 2 (I-R/Untreated), unilateral testicular torsion was performed for 6 h followed by 1 h of detorsion of the testis. In group 3 (I-R/Dex), after performing the same procedures as in group 2, dexmedetomidine was given intravenously. Ipsilateral orchidectomies were performed in all experimental rats for histological examination. The levels of MDA and activities of SOD and CAT were measured in the testicular tissue. Results. MDA levels were higher in group 2 than in group 1 rats and lower in group 3 than in group 2. SOD and CAT activities were higher in group 3 than in group 2 rats. Histopathologically, in the group 2 rats the lesions varied between grades III and IV and edema, congestion, hemorrhage between seminiferous tubules, and necrosis of the germinal cells were predominant features in sections. However, most of the specimens in the dexmedetomi-dine-treated group 3 showed grades I and II injury. The testicular injury score was also lower in group 3 rats than in group 2. Conclusions. The results show that dexmedetomidine may play a protective role in reducing injury caused by I-R (Adv Clin Exp Med 2008, 17, 5, 513-518).Öğe Biliyer askariazisin neden olduğu tıkanma ikteri: Bir akut karın olgusu(2009) Öztürk, Hayrettin; Öztürk, Hülya; Duran, Hatun; Otçu, SelçukAscaris lumbricoides dünyadaki en yaygın helmintik hastalıklardan biri olup birçok komplikasyon oluşturabilir. Helmintin safra yolları içinde varlığının gösterilmesi, özellikle çocuklarda, nadir bir durumdur. Bu yazıda, cerrahi akut karın bulgularına benzer şikayetlerle başvuran ve safra yolları içinde askaris helminti gösterilen bir olgu sunuldu. Ultrasonografik değerlendirme ile tanısı konan hastaya, tedavide mebendazol ve antispazmolitik verilerek tedavi sağlandı. Biliyer askariazis çocuk yaş gurubu hastalar dahil olmak üzere endemik bölgelerden gelen hastalarda görülen akut karın ağrısı tablosunda ayırıcı tanıda düşünülmelidir.Öğe Bir adolesanda memede cystosarcoma phyllodes: Olgu sunumu(2009) Öztürk, Hülya; Öztürk, Hayrettin; Yılmaz, Fahri; Uyan, Ayten Pamukçu; Aktaş, EzgiCystosarcoma phyllodes (CP) çocuklarda nadir görülür. Bu olgu sunumunda 13 yaşında bir kız çocuğunda memenin CP tümörü tanımlandı. Klinik muayenede hızlı olarak büyüyen ağrısız tümör mevcuttu. Kitle çıkarıldıktan sonra yapılan histolojik incelemede hiperselüler stroma ve stroma içine uzanan yaprak benzeri glandlar saptandı. Cystosarcoma phyllodesin tek etkili tedavisi, normal meme dokusu sınırlarının sağlandığı geniş cerrahi eksizyondur.Öğe Bir yenidoğanda ekstralober pulmoner sekestrasyon(2014) Öztürk, Hülya; Bilir, Sevil Göksügür; Dağıstan, Emine; Yılmaz, Fahri; Öztürk, HayrettinKonjenital Pulmoner Sekestrasyon (KPS) fonksiyon görmeyen primitif dokudan meydana gelen solid veya kistik kitle şeklinde görülebilen bir torasik malformasyondur. Trakeobronşiayal ağaçla bağlantısı yoktur ve alışılmadık bir sistemik kan dolaşımına sahiptir. Çeşitli konjenital mal- formasyonlar KPS'a eşlik edebilir. Olguların yarıdan çoğuna ileri çocukluk dönemi hatta erişkinlik döneminde tanı konulmaktadır. Yenidoğanlar ve çocuklar genellikle asemptomatiktir. Bu grupta tanı genellikle diğer ano- malilerin varlığından dolayı konmaktadır. Burada solunum sıkıntısı ile kliniğimize kabul edilen 5 günlük yenidoğan sunulmaktadır. Başka hiç bir malformasyonu bulunmayan hastamıza KPS tanısı konulmuştur.Öğe Can inferior vena cava diameter and collapsibility index be a predictor in detecting preoperative intravascular volume change in pediatric patients?(Sciendo, 2021) Coşgun, Zeliha; Dağıstan, Emine; Coşgun, Mehmet; Öztürk, HayrettinBackground: Inferior vena cava (IVC) ultrasound measurement is a reliable indicator used in the assessment of intravascular volume status. The aim of this study was to evaluate intravascular volume changes in pediatric patients by measuring the IVC diameter and collapsibility index (CI) in children whose oral feeding was restricted preoperatively. Material and Methods: From May 2018 to October 2018, a total of 55 pediatric patients who were scheduled for surgery were included in this prospective, observational, cohort study. Fasting and satiety IVC diameters and CIs of patients were determined by ultrasonographic evaluation twice: in the preoperative preliminary evaluation, when the patients were satiated, and before surgery, during a fasting period of 6-8 hours. Ultrasonographic data were recorded and compared between fasting and satiety periods. Results: In the grey scale (B-mode), mean IVC diameter was significantly higher when the patients were satiated, compared to the measurements made just before surgery during the fasting period. In the M-mode, the mean IVC diameter was significantly higher only during the inspiratory phase when the patients were satiated, while during the expiratory phase it was detected to be statistically similar. Mean CI was significantly higher in the immediate preoperative period, compared to the assessment made when satiated. Conclusion: Preoperative ultrasound IVC diameter and CI measurement can be a practical and useful method for evaluating preoperative intravascular volume in children.Öğe Carvacrol attenuates histopathogic and functional impairments induced by bilateral renal ischemia/reperfusion in rats(Elsevier France-Editions Scientifiques Medicales Elsevier, 2018) Öztürk, Hülya; Çetinkaya, Ayhan; Düzcü, Selma Erdoğan; Tekçe, Buket Kın; Öztürk, HayrettinBackground: Renal ischemia/reperfusion injury is the most common cause of acute kidney injury, which frequent occurrence in critically ill patients. Objectives: The aim of this study was to investigate the role of Carvacrol (CARV) against bilateral ischemia reperfusion (I/R) in rats. Methods: Renal I/R injury were induced by clamping of the left and right renal arteries for 45 min followed by 24 h of reperfusion. Thirty male Sprague-Dawley rats were randomly allocated to three groups (n = 10): the sham-control group, the renal I/R-untreated (I/R-untreated) group, and the I/R- CARV-treated group. At 2 h before reperfusion, the rats in the I/R- CARV - treated group rats received an i.p. injection of 75 mg/kg CARV. Renal function and histological changes were compared and the relevant parameters of oxidative stress and inflammation were detected. Results: Compared to the sham-control group, I/R led to renal dysfunction as evidenced by higher plasma urea and creatinine along with increase in oxidative stress and histological changes in renal tissues. Treatment with CARV decreased urea, creatinine, and renal MDA and MPO levels, and increased SOD, CAT, GSH activity and eNOS expression in the kidney. In the I/R-CARV-treated group, minimal hydropic changes in the tubular epithelial cells and regeneration of tubular epithelium were observed. Conclusion: These results suggest that CARV treatment could reduce renal injury induced by bilateral renal I/R via anti-inflammatory, antioxidant and cytoprotective effects.Öğe Carvacrol reduces the severity of intestinal mucosal damage caused by intestinal ischemia - Reperfusion in rats(Soc Stiinte Farmaceutice Romania, 2019) Öztürk, Hülya; Çetinkaya, Ayhan; Düzcü, Selma Erdoğan; Yis, Özgür Mehmet; Öztürk, HayrettinIntestinal ischemia-reperfusion (I/R) is a pathophysiological process that is common in many clinical conditions such as shock, sepsis, mesenteric thrombosis, necrotizing enterocolitis, and bowel transplantation. Our aim in this study was to investigate the potential protective effects of carvacrol on the intestinal I/R injury in a rat model. The 30 rats were randomly divided into three groups (n = 10): The sham-control (group 1) underwent only the separation of the superior mesenteric artery but not the occlusion. In the FR-untreated (group 2) and I/R-carvacrol-treated groups (group 3), the superior mesenteric artery was clamped for 45 min, followed by 60 min of reperfusion. 2 hours before ischemia, the group 3 of rats received an intraperitoneal injection of carvacrol at a dose of 75 mg/kg bw. At the end of the experiment, intestinal tissue samples were taken for oxidative stress assessment including superoxide dismutase (SOD), malondialdehyde (MDA), myeloperoxidase (MPO), total antioxidant status (TAS) and total oxidant status (TOS). In addition, the intestine sections were stained with haematoxylin-eosin to evaluate morphological changes and immunohistochemical staining was performed for inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) assessment. The intestinal mucosa was significantly damaged in the group 2, which was markedly attenuated after carvacrol treatment. The tissue MDA, MPO and TOS content increased significantly in the group 2, but they were reduced by carvacrol treatment. In addition, SOD and TAS activity increased markedly in group 3 as compared to group 2. Immunohistochemical staining showed that iNOS increased and eNOS decreased in group 2, which was improved in reverse direction after carvacrol treatment. Carvacrol may be a potential therapeutic agent for the treatment of intestinal I/R injury.Öğe Çocuklarda hidatik hastalık: 49 olgunun analizi(2006) Öztürk, Hülya; Öztürk, Hayrettin; Otçu, Selçuk; Önen, Abdurrahman; Duran, HatunAmaç: Çalışmadaki amacımız hidatik hastalık tanısı koyulan ve tedavisi yapılan olgularımızın tanı ve tedavi özelliklerini belirlemek, morbidite ve mortalite açısından değerlendirmektir. Yöntem: Kliniğimizde Echinococcus granulosus'e bağlı hidatik hastalık tanısı konulan ve tedavisi yapılan 49 hasta değerlendirildi. Hastalar yaş, cinsiyet, semptomların başlamasından sonraki geçen süre, semptom ve bulgular, tanı metodları, kistin lokalizasyonu, çapı, sayısı, tedavi yöntemleri, mortalite, morbidite ve nüks açısından değerlendirildi. Tanı ultrasonografi, tomografi, Casoni ve Weinberg testleri ile konuldu. Ameliyat sonrası dönemde 27 hastaya Albendezol tedavisi verildi. Bulgular: Hastaların 24'ü erkek, 25'i kız ve yaş ortalaması 10.5 yıl idi. En sık başvuru şikayeti karında ağrı idi. Kist 27 olguyla (%67) en sık karaciğerde lokalizeydi. Kistler açık cerrahi ve perkütan yolla tedavi edildi. Hastaların 47'sinde (%96) kistektomi, kapitonaj ve drenaj uygulandı. Perkütan tedavi 2 (%4) hastada uygulandı. Ameliyat sonrası ölen hastamız olmadı. Ortalama 24 aylık (8-60 ay) takip sürecinde %4 oranında nüks gelişti. Sonuç: Sonuç olarak çocuklarda cerrahi girişimle kist hidatiklerinin tedavisi başarılı olmaktadır. Ayrıca, hidatik hastalık bölgemizde yaygın olarak devam etmektedir.Öğe Çocuklarda konjenital musküler tortikollis: Geç başvuran 12 olgunun analizi(2008) Öztürk, Hayrettin; Okur, Mehmet Hanifi; Öztürk, Hülya; Çiğdem, Murat Kemal; Duran, Hatun; Önen, Abdurrahman; Dokucu, Ali İhsanAmaç: Bu çalışmadaki amacımız geç başvuran konjenital musküler tortikollis (KMT)’li hastalarımızın klinik, tanı ve tedavi özelliklerini analiz etmek ve literatür ile karşılaştırmaktır. Yöntem: Retrospektif olarak hastalarımız yaş, cinsiyet, klinik bulgular, yüzde ikincil biçim değişiklikleri, şişliğin yerleşimi, tanı yöntemleri, ek anomaliler, cerrahi girişimde karşılaşılan bulgular, sağaltım amacıyla uygulanan cerrahi girişimler açısından değerlendirildi. Bulgular: Hastalarımızın (5 erkek, 7 kız) yaş ortalaması 6,7 (1,5-12 yaş) yıl idi. Lezyonların çoğunluğu solda (%58) görüldü. Olgularımızın ikisi küçük yaş grubundaydı. İki olguda (%17) boyunda kitle palpe edilebildi. Olguların çoğunluğunda boyun hareketlerinde kısıtlılık (%83) en sık bulgu idi. Serimizdeki hastaların tümü cerrahi girişimle tedavi edildi ve ayrıca fizik-tedavi egzersizleri de uygulandı. Sonuç: Bu çalışmada, geç başvuran KMT’li çocuklarımızın çoğunluğunda cerrahi tedavi ve konservatif tedavi ile boyun hareketleri ve boyun eğriliğinde belirgin bir düzelme oldu.Öğe A comprehensive analysis of 51 neonates with congenital intestinal atresia(Saudi Med J, 2007) Öztürk, Hayrettin; Öztürk, Hülya; Gedik, Şenol; Duran, Hatun; Önen, AbdurrahmanObjective: To determine contemporary patterns of presentation and trends in the management and outcome of 51 newborn infants with intestinal atresia. Methods: We retrospectively reviewed 51 cases of intestinal atresia between January 1983 and February 2003. Clinical data included antenatal history, age, gender, weight, presenting symptoms and signs, diagnostic procedures, location and type of atresia, associated abnormalities, surgical treatment, associated problems, morbidity, mortality and plans of treatment. Results: Twenty children had duodenal obstruction, 24 had jejunoileal atresia, and 7 had colonic atresia. Approximately one-fourth of patients associated with duodenal atresia. had preterm delivery and all patients with jejunoileal and colonic atresia were full term. Clinical features such as vomiting, abdominal distention, delayed meconium passage and jaundice were more frequent in jejunoileal atresia patients. Other associated organ anomalies particularly Down's syndrome were more frequent in duodenal atresia patients. A duodeno-duodenostomy was preferred in most of the patients with duodenal atresia and annular pancreas; duodenotomy and web excision for those with duodenal webs; and resection with end-to-end anastomosis for those with jejunoileal atresia. In all patients with colonic atresia, colostomy procedure was performed as the first step of surgery. Conclusion: Experienced neonatal care and prompt total parenteral. nutrition by placing central line during surgery may improve the outcome of such patients.Öğe Continuous infusion of small-volume fluid resuscitation in the treatment of combined uncontrolled hemorrhagic shock and Head Injury(College Physicians & Surgeons Pakistan, 2007) Öztürk, Hayrettin; Yağmur, Yusuf; Taş, Aşkın; Topçu, Soykan; Orak, MuratObjective: To determine-the effect of continuous limited fluid resuscitation on the hemodynamic response and survival in rats in a model of uncontrolled hemorrhage shock due to Massive Splenic Injury (MSI) and Head Injury (HI). Design: An experimental study. Place and Duration of Study: Dicle University Animal Research Laboratory, Turkey, between January and February 2005. Subjects and Methods: Seventy Sprague-Dawley rats were used in this study. Group 1 rats (n=10) was sham-operated. In group 2 (n=10), only Massive Splenic Injury (MSI) was performed and untreated. In group 3 (n=10), only head injury (HI) was performed and untreated. In group 4 (n=10), HI and MSI were performed and were untreated. In group 5 (n=10), HI and MSI were performed and 15 minutes later treated with 7.5% NaCl. In group 6 (n=10), HI and MSI were performed, and rats were treated with Ringer's Lactate (RL) solution. In group 7 (n=10), HI and MSI were performed, rats were treated with 0.9% NaCl. In groups 2,4,5,6 and 7 midline incision was reopened and splenectomy was performed at 45 minutes. Results: In group 4 rats, Mean Arterial Pressure (MAP) was decreased from 104 +/- 6.1 mmHg to 75 +/- 19.5 mmHg at 15 minutes; heart rate decreased from 357 +/- 24.9 beats/min to 321 +/- 62.1 beats/min and hematocrit decreased from 46 +/- 1.3% to 43 +/- 2.5% (p<0.01). Similar early changes in MAP, heart rate and hematocrit were observed in groups 5, 6, and 7, at 15 minutes. At 45,60 and 120 minutes, in fluid resuscitated rats (group 5,6,7) MAP, heart rate and hematocrit values were measured higher than group 2 and 4 (p<0.01 for all). At 120 min. in group 6, hematocrit was higher than group 4, 5 and 7, in group 6, total blood loss after splenectomy was calculated at 20 +/- 2.4% of blood volume and was the best value compared to other fluid resuscitated group 5 and 7 (28% and 27% of blood volume) (p<0.01). Mortality was lower in all fluid resuscitated groups when compared to group 3 and 4 (p<0.05). The median survival time was again higher in fluid resuscitated groups. Conclusion: Continuous infusion of 7.5% NaCl, RL and 0.9% NaCl following uncontrolled hemorrhagic shock with massive splenic injury and combined head injury resulted in better survival and RL did not increase abdominal bleeding before splenectomy was performed.Öğe Damak yarığı olgularının tedavisinde Glubran 2’nin kullanımı(2008) Öztürk, Hülya; Öztürk, Hayrettin; Duran, Hatun; Okur, Mehmet HanifiAmaç: Yarık damak ve dudak insanlardaki en sık konjenital kraniyofasyal anomalidir. Palatoplastinin gecikmiş komplikasyonu fistül gelişimi ve midfasiyal büyüme geriliğidir. Bu çalışmada fistül gelişiminin engellenmesinde doku yapıştırıcılarının kullanımının etkinliğinin araştırılması amaçlanmıştır. Gereç ve yöntem: Ocak 1995 ile Mayıs 2005 tarihleri arasında kliniğimizde opere edilen 39 hasta yaş, cinsiyet, yarık damağın tipi, operasyon tekniği, doku yapıştırıcısı kullanımı ve komplikasyon gelişimi bakımından karşılaştırıldı. Bulgular:Yarık damaklı 39 hastanın 24’üne palatoplasti, 15 hastayada palatoplastiye ek olarak doku yapıştırıcısı Glubran 2 uygulandı. Yalnızca palatoplasti yapılan 24 hastanın 7’sinde (%29.1) fistül gelişti. Ancak palatoplastiye ek olarak Glubran 2 kullanılan 15 hastanın 1’inde (%6.6) fistül gelişti Sonuç: Yarık damaklı hastalarımızın tedavisindeki bu olumlu sonuçlarında klinik tecrübelerimizdeki gelişmelere ek olarak doku yapıştırıcısı Glubran 2’nin etkili olduğu söylenebilir.Öğe Dartos flap coverage of the neourethra following repair for primary hypospadias, reoperative hypospadias and urethrocutaneous fistulas. It is a safe approach(Acta Cirurgica Brasileira, 2010) Öztürk, HayrettinPurpose: Urethrocutaneous fistula and neourethral dehiscence are frequently seen complications of hypospadias surgery requiring reoperation. In this study we report the experience of one surgeon with dartos flap coverage in primary hypospadias, reoperative hypospadias and urethrocutaneous fistulas repair. Methods: A total of 23 patients underwent hypospadias and urethrocutaneous fistulas repair from January 2006 to May 2009. Fourteen patients were operated on for primary hypospadias repair at our institution and 9 patients were admitted for hypospadias complications such as failed hypospadias repair and urethrocutaneous fistula. In all the patients, the dartos flap was dissected and transposed to cover the neourethra. Operative results were recorded. Results: The primary surgical procedure was a one-stage repair in 61% (n = 14); tubularised incised plate (TIP) urethroplasty in 43% (n = 6) and a Mathieu procedure in 57% (n = 8). Urethrocutaneous fistulas complicating the previous initial hypospadias repair were anterior in 33% (n = 2), middle in 33% (n = 2) and proximal in 33% (n = 2). Repair of the fistula was successful on the first attempt in all patients. The reason for redo surgery in 3 patients was complete dehiscence and the patients had distal shaft hypospadias. Conclusion: Dartos flap coverage of the neourethra seems to be an effective method of reducing the fistulous complication rate following primary and secondary hypospadias repair.Öğe Differential expression of CD34, S100, and c-Kit in interstitial cells of cajal in infantile hypertrophic pyloric stenosis - immunochemical study(Wroclaw Medical Univ, 2009) Öztürk, Hülya; Öztürk, Hayrettin; Yılmaz, Fahri; Okur, Hanifi; Otcu, Selçuk; Dokucu, Ali İhsanBackground. The pathogenesis of infantile hypertrophic pyloric stenosis (IHPS) is poorly understood although many hypotheses have been proposed. Objectives. Assessment whether the differential expression of c-Kit, CD34, and S100 may be involved in the development of IHPS. Material and Methods. Specimens from 14 infants with IHPS and seven control subjects were immunohistochemically stained for c-Kit, CD34, and S100. The numbers of CD34(+), S100(+), and c-Kit(+) cells in five random fields per specimen were compared via light microscopy (x200). Results. In normal pyloric tissue, specific and intense c-Kit immunoreactivity was observed in the muscle layers and moderate staining was observed around the myenteric plexus. In IHPS patients, c-Kit+ cells were either absent or markedly reduced around the myenteric plexus. In control and IHPS patients, CD34(+) cells were not observed around the myenteric plexus. In the vascular endothelium, moderate CD34 staining was observed in specimens from control subjects, whereas intense staining was observed for IHPS patients. In normal pyloric tissue, moderate S100 immunoreactivity was observed in the muscle layers and intense staining was observed in the myenteric plexus. In IHPS patients, few S100(+) cells were observed in the pyloric muscle layers and S100 immunoreactivity decreased markedly around the myenteric plexus. Conclusions. These results suggest that the numbers of c-Kit(+) and S100(+) cells are markedly decreased in the pyloric muscle layers and around the myenteric plexus in IHPS patients. Thus a lack of c-Kit and S100, but not CD34, expression may be a critical factor in the pathogenesis of IHPS and may serve as a useful prognostic tool in the treatment of this disease (Adv Clin Exp Med 2009, 18, 1, 33-39).Öğe Distal ileal perforation secondary to ingested foreign bodies(Coll Physicians & Surgeons Pakistan, 2009) Yağmur, Yusuf; Öztürk, Hayrettin; Öztürk, HülyaA 22-year-old man was admitted with abdominal pain, nausea and vomiting secondary to ingestion of multiple foreign bodies. He was found to be in increasing distress with an increase in abdominal pain and distention and no passage of foreign bodies. Patient underwent a laparotomy. Foreign bodies removed from perforated distal ileum included 8 big size (10 cm) plastic clothes pegs, a 10 cm pencil, couple of stones, a 10 cm wood nail, nail scissors and a small size battery. In case of foreign body ingestion, especially in mentally-ill patients, the patient should be carefully examined because of the potential risk of obstruction and bowel perforation, more so, if the foreign body is a battery which can puncture causing corrosive injury as well.Öğe Dose-related effects of dexamethasone on liver damage due to bile duct ligation in rats(Baishideng Publishing Group Inc, 2006) Eken, Halil; Öztürk, Hayrettin; Öztürk, Hülya; Büyükbayram, HüseyinAIM: To evaluate the effects of dexamethasone on liver damage in rats with bile duct ligation. METHODS: A total of 40 male Sprague-Dawley rats, weighing 165-205 g, were used in this study. Group 1 (sham-control, n = 10) rats underwent laparotomy alone and the bile duct,was just dissected from the surrounding tissue. Group 2 rats (untreated, n = 10) were subjected to bile duct ligation (BDL) and no drug was applied. Group 3 rats (low-dose dexa, n = 10) received a daily dose of dexamethasone by orogastric tube for 14 d after BDL. Group 4 rats (high-dose dexa, n = 10) received a daily dose of dexamethasone by orogastric tube for 14 d after BDL. At the end of the two-week period, biochemical and histological evaluations were processed. RESULTS: The mean serum bilirubin and liver enzyme levels significantly decreased, and superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) values were significantly increased in low-dose dexa and high-dose dexa groups when compared to the untreated group. The histopathological score was significantly less in the low-close and high-dose dexa groups compared to the untreated rats. In the low-dose dexa group, moderate liver damage was seen, while mild liver damage was observed in the high-dose dexa group. CONCLUSION: Corticosteroids reduced liver damage produced by bile duct obstruction. However, the histopathological score was not significantly lower in the high-dose corticosteroid group as compared to the low-dose group. Thus, low-dose corticosteroid provides a significant reduction of liver damage without increased side effects, while high dose is associated not with lower fibrosis but with increased side effects.Öğe Effect of a 50-Hz sinusoidal electromagnetic field on the integrity of experimental colonic anastomoses covered with fibrin glue(Wroclaw Medical Univ, 2009) Girgin, Sadullah; Öztürk, Hayrettin; Gedik, Ercan; Akpolat, Veysi; Kale, Ebru; Öztürk, HülyaBackground. Low-frequency magnetic fields have been shown to affect biological processes. In this article the effects of 50-Hz sinusoidal magnetic field (MF) stimulation and application of fibrin glue on the healing of experimental colonic anastomoses were investigated. Material and Methods. Twenty-eight rats were divided into four groups. Group 1 underwent 2-cm left colonic resection and primary anastomosis. Group 2 underwent normal resection anastomosis and the area was covered with fibrin glue. Group 3 underwent normal resection anastomosis and the rats were exposed to a 50-Hz sinusoidal MF. Group 4 underwent normal resection anastomosis, the anastomosis area was covered with fibrin glue, and the rats were exposed to a 50-Hz sinusoidal MF. Investigations included bursting pressure measurement, hydroxyproline content, and histopathological changes. Results. Tissue hydroxyproline levels and anastomotic bursting pressures of groups 2, 3, and 4 were significantly higher than in group 1. Collagen deposition and fibroblast infiltration in groups 2, 3, and 4 had higher scores than in group 1. Furthermore, these results were significantly higher in group 4 rats than in the other groups. Histopathological examination of the anastomosis revealed significantly better healing patterns for group 4 than for groups 1, 2, and 3. Conclusions. A50-Hz sinusoidal MF stimulation and application of fibrin glue provided a significant gain in anastomotic healing in the large intestine. A combination of a 50-Hz sinusoidal MF and fibrin glue has significantly favorable effects on healing of experimental colon anastomosis (Adv Clin Exp Med 2009, 18, 1, 13-18).Öğe Effect of dexmedetomidine on ischemia-reperfusion injury in rat kidney: a histopathologic study(Taylor & Francis Inc, 2009) Koçoğlu, Hasan; Öztürk, Hülya; Öztürk, Hayrettin; Yılmaz, Fahri; Gülcü, NebahatIschemia-reperfusion (I-R) injury remains the leading cause of acute renal failure. The purpose of this experimental study was to determine the role of dexmedetomidine on histologic alterations induced by renal I-R in rats. In the present study, thirty male Sprague-Dawley rats weighing 200-220 g were randomly assigned into three groups: the sham-control group (group 1, n = 10), the R/untreated group (group 2, n = 10), and the I-R/dexmedetomidine-treated group (group 3, n = 10). For group one, we performed a sham operation. The abdomen was dissected, the right kidney was harvested, and then the left renal pedicle exposed. Renal clamping was not applied. For group 2, rats underwent left renal ischemia for 60 minutes followed by reperfusion for 45 minutes. For group 3, the same surgical procedure as in group 2 was performed, and dexmedetomidine (100 g/kg, intraperitoneal) was administrated at the starting time of reperfusion. The rats were sacrificed after reperfusion, and the kidney tissue was harvested. The histopathological score in the kidney of the I-R/dexmedetomidine-treated group rats was significantly lower than that of I-R/untreated group rats. This score in I-R/untreated group rats was higher than the other two groups, which was statistically significant. In the I-R/untreated group rats, kidneys of untreated ischemia rats showed tubular cell swelling, cellular vacuolization, pyknotic nuclei, medullary congestion, and moderate to severe necrosis. Treatment with dexmedetomidine shows normal glomeruli and slight edema of the tubular cells. These findings provide the first evidence that dexmedetomidine can reduce the renal injury caused by I-R of the kidney, and may be useful in enhancing the tolerance of the kidney against renal injury.
