The Role of Gi and Gs Proteins in Hypoxic Vasoconstriction of Lamb Isolated Pulmonary Artery Rings

Basit Öğe Kaydı
dc.authoridsilan, coskun/0000-0002-8352-6571
dc.contributor.authorErbas, Mete
dc.contributor.authorBalbay, Oner
dc.contributor.authorUzun, Ozge
dc.contributor.authorGulec Balbay, Ege
dc.contributor.authorSilan, Coskun
dc.date.accessioned2024-09-25T19:58:39Z
dc.date.available2024-09-25T19:58:39Z
dc.date.issued2014
dc.departmentAbant İzzet Baysal Üniversitesien_US
dc.description.abstractHypoxic pulmonary vasoconstriction (HPV) is an intrapulmonary adaptive mechanism that matches alveolar ventilation to perfusion. However during prolonged alveolar hypoxia HPV occurs with many pulmonary diseases. Despite intensive studies, cellular mechanisms of HPV are still not well defined. G proteins are a family of membrane-associated proteins believed to be involved in the transduction of various signals including the regulation of vascular tonus. In this study, we aimed to determine the contribution of G(i) and G(s) proteins in hypoxic vasoconstriction of lamb isolated pulmonary artery rings. Pulmonary arteries were isolated from left lower lobe of freshly slaughtered lamb. Arteries suspended in an organ bath filled with Krebs-Henseleit solution and isometric contraction recorded continuously via an isometric transducer connected to a computerised polygraphy system. The solution aerated with 75% N-2 - 20% O-2 - 5% CO2 (normoxic) and 95% N-2 - 5% CO2 (hypoxic) pO(2) of bathing medium was measured continuosly using an oxygen electrode. Pertussis toxin and cholera toxin were used to investigate the role of G(i) and G(s) proteins. In the present study, we observed that hypoxia had no effect on resting force in large artery rings, but it caused a further contraction (1.7 +/- 0.5 mN/mm(2), n=10) in 3 mu M 5-HT precontracted pulmonary arteries rings. Hypoxic vasoconstriction was inhibited by preincubation with 2 mu g/ml cholera toxins (from 2.6 +/- 0.4 mN/mm(2), to 1.0 +/- 0.4 mN/mm(2), n=6) and potentiated by preincubation with pertussis toxins (2 mu g/ml) (from 0.6 +/- 0.4 mN/mm(2), to 1.7 +/- 0.3 mN/mm(2), n=6). These results indicate that signal transduction mediated by G(i) and G(s) proteins may be an important mechanism in the hypoxic vasoconstriction in lamb isolated large pulmonary arteries.en_US
dc.identifier.doi10.9775/kvfd.2013.10040
dc.identifier.endpage285en_US
dc.identifier.issn1300-6045
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-84891654248en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage279en_US
dc.identifier.urihttps://doi.org/10.9775/kvfd.2013.10040
dc.identifier.urihttps://hdl.handle.net/20.500.12491/13688
dc.identifier.volume20en_US
dc.identifier.wosWOS:000333080000018en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isotren_US
dc.publisherKafkas Univ, Veteriner Fakultesi Dergisien_US
dc.relation.ispartofKafkas Universitesi Veteriner Fakultesi Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzYK_20240925en_US
dc.subjectHypoxiaen_US
dc.subjectPulmonary vasoconstrictionen_US
dc.subjectCholera toxinsen_US
dc.subjectPertussis toxins G-proteinsen_US
dc.subjectLamben_US
dc.titleThe Role of Gi and Gs Proteins in Hypoxic Vasoconstriction of Lamb Isolated Pulmonary Artery Ringsen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu