Enhanced reducing sugar production and extraction for Chlorella vulgaris in mixotrophic cultivation using high hydrostatic pressure processing and ultrasound

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dc.authorid0000-0003-1050-8206en_US
dc.authorid0000-0003-2242-0703en_US
dc.authorid0000-0001-5064-4195en_US
dc.contributor.authorUzuner, Sibel
dc.contributor.authorKurhan, Şebnem
dc.contributor.authorEvrendilek, Gülsün Akdemir
dc.date.accessioned2024-07-26T12:21:59Z
dc.date.available2024-07-26T12:21:59Z
dc.date.issued2024en_US
dc.departmentBAİBÜ, Mühendislik Fakültesi, Gıda Mühendisliği Bölümüen_US
dc.description.abstractAlthough extraction of polysaccharides to convert reducing sugars (RS) from microalgae by acid or alkali pretreatments and enzymatic hydrolysis has been extensively studied, few reports exploring the use of high hydrostatic pressure processing (HHP) and ultrasonication (US) as emerging technologies for the extraction of sugars from microalgae biomass exist. Thus, the present study was conducted to determine the effects of mixotrophic growth and stress conditions (NaNO3 and CO2 concentration and light intensity) on RS and protein accumulation in the unicellular green alga Chlorella vulgaris in addition to optimization of the effectiveness of the sequential applications of HHP and US with dilute acid as well as simultaneous enzymatic saccharification on the production of RS from microalga cells. High light intensity, high CO2 concentration and limited nitrogen concentration promoted RS production. The maximum protein content (0.0683 mg g(-1)) was achieved at 0.3 g l(-1) NaNO3 concentration, 7000 mu mol photons m(-2) s(-1) and 6 l min(-1) CO2 concentration. The highest RS content of C. vulgaris after 48 h enzymatic saccharification (583.86 +/- 13.23 mg g(-1)) was obtained at 1% (w/w) acid concentration and 80% amplitude for 30 min with 79.4% RS yield. Combined US-assisted dilute acid pretreatment and enzymatic hydrolysis were also found to be more effective than HHP assisted dilute acid pretreatment and enzymatic saccharification. Therefore, microalgal biomass can be considered a suitable renewable feedstock used in fermentation. Highlights center dot The cultivation period of Chlorella vulgaris was reduced from 25 days to 14 days using mixotrophic growing conditions.center dot Mixotrophic conditions enhanced reducing sugar productivity.center dot Novel extraction techniques enhanced the extraction of reducing sugar from microalgae.en_US
dc.description.sponsorshipThe authors would like to thank the Innovative Food Technologies Development Application and Research Center (YENIGIDAM) of Bolu Abant Izzet Baysal University (BAIBU) for HHP analyses, and I. Isci for his help with construction of the lab-scale photobiorea; Innovative Food Technologies Development Application and Research Center (YENIGIDAM) of Bolu Abant Izzet Baysal Universityen_US
dc.identifier.citationUzuner, S., Kurhan, S., & Akdemir Evrendilek, G. (2024). Enhanced reducing sugar production and extraction for Chlorella vulgaris in mixotrophic cultivation using high hydrostatic pressure processing and ultrasound. European Journal of Phycology, 59(1), 82-93.en_US
dc.identifier.doi10.1080/09670262.2023.2248216
dc.identifier.endpage93en_US
dc.identifier.issn0967-0262
dc.identifier.issn1469-4433
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85173767218en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage82en_US
dc.identifier.urihttp://dx.doi.org/10.1080/09670262.2023.2248216
dc.identifier.urihttps://hdl.handle.net/20.500.12491/12278
dc.identifier.volume59en_US
dc.identifier.wosWOS:001081807100001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorEvrendilek, Gülsün Akdemir
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofEuropean Journal of Phycologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHigh Hydrostatic Pressure Processingen_US
dc.subjectLight Intensityen_US
dc.subjectMicroalgaen_US
dc.subjectScenedesmus-Obliquusen_US
dc.subjectStarchen_US
dc.subjectAccumulationen_US
dc.titleEnhanced reducing sugar production and extraction for Chlorella vulgaris in mixotrophic cultivation using high hydrostatic pressure processing and ultrasounden_US
dc.typeArticleen_US

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