Effects of exogenous auxin and auxin transport inhibitor on somatic embryogenesis and analysis of cardenolides in Digitalis lamarckii Ivan

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dc.authorid0000-0001-6262-2866en_US
dc.authorid0000-0002-6786-3973
dc.contributor.authorVerma, Sandeep
dc.contributor.authorYücesan, Buhara
dc.contributor.authorGürel, Ekrem
dc.date.accessioned2021-06-23T19:26:54Z
dc.date.available2021-06-23T19:26:54Z
dc.date.issued2010
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.description7th Tannin Conference/58th International Congress and Annual Meeting of the Society-for-Medical-Plant-and-Natural-Product-Research -- AUG 29-SEP 02, 2010 -- Berlin, GERMANYen_US
dc.description.abstractDigitalis lamarckii (Plantaginaceae), is an endemic species of the Turkish flora. D. lamarckii are medicinally important plants as they contain cardiac glycosides that strengthen the cardiac diffusion and regulate the heart rhythm [1]. Hypocotyl sections excised from 21days old seedlings of D. lamarckii were used to test the effect of indole-3-acetic acid (IAA) on direct somatic embryogenesis. Although direct somatic embryogenesis was obtained with all IAA concentrations (0.1, 0.5 and 1mg/l) from the upper part of hypocotyl explants (Fig.1), optimum results were achieved with 1mg/l IAA. Several studies have shown that proper polar transport of auxin is a pre-requisite for normal embryogenesis beyond the globular stage [2]. The auxin transport inhibitor 2,3,5-triiodobenzoic acid (TIBA) blocks morphological transitions to subsequent stages; for example, they cause the formation of enlarged globular and oblong embryos. We observed morphologically abnormal embryos developed on media supplemented with auxin polar transport inhibitors (0.1–2mg/l IAA with 1–5mg/l TIBA). TIBA disrupts somatic embryogenesis and thus specific spatial auxin distribution due to auxin movement may be important in establishing somatic embryo development in D. lamarckii. In the present investigation, contents of five cardenolides as well the total cardenolide contents of the direct somatic embryos were determined, the amount of total cardenolides being 380mg/kg. It has been reported that somatic embryos of the globular stage turned green and, concomitantly, started to produce cardiac glycosides in Digiltalis lanata [3,4].en_US
dc.description.sponsorshipSoc Med Plant Nat Prod Resen_US
dc.identifier.endpage1381en_US
dc.identifier.issn0032-0943
dc.identifier.issue12en_US
dc.identifier.startpage1381en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12491/6685
dc.identifier.urihttps://www.thieme-connect.com/products/ejournals/abstract/10.1055/s-0030-1265856
dc.identifier.volume76en_US
dc.identifier.wosWOS:000282066500799en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.institutionauthorVerma, Sandeep
dc.institutionauthorYücesan, Buhara
dc.institutionauthorGürel, Ekrem
dc.language.isoenen_US
dc.publisherGeorg Thieme Verlag Kgen_US
dc.relation.ispartofPlanta Medicaen_US
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDigitalis lamarckii Ivanen_US
dc.titleEffects of exogenous auxin and auxin transport inhibitor on somatic embryogenesis and analysis of cardenolides in Digitalis lamarckii Ivanen_US
dc.typeConference Objecten_US

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