Characterization of fungal RTG2 genes in retrograde signaling of Saccharomyces cerevisiae

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dc.authorid0000-0003-0097-1125
dc.authorid0000-0001-9436-7337
dc.contributor.authorÜnlü, Ercan Selçuk
dc.contributor.authorNarayanan, Lakshmi
dc.contributor.authorGordon, Donna M.
dc.date.accessioned2021-06-23T18:55:49Z
dc.date.available2021-06-23T18:55:49Z
dc.date.issued2013
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Kimya Bölümüen_US
dc.description.abstractChanges in the functional status of mitochondria result in the transcriptional activation of a subset of nuclear-encoded genes in a process referred to as retrograde signaling. In Saccharomyces cerevisiae, this molecular link between mitochondria and the nuclear genome is controlled by three key signaling proteins: Rtg1p, Rtg2p, and Rtg3p. Although the retrograde signaling response has been well characterized in S. cerevisiae, very little is known about this pathway in other fungi. In this study, we selected four species having uncharacterized open reading frames (ORFs) with more than 66% amino acid identity to Rtg2p for further analysis. To determine whether these putative RTG2 ORFs encoded bona fide regulators of retrograde signaling, we tested their ability to complement the defects associated with the S. cerevisiae rtg2? mutant. Specifically, we tested for complementation of citrate synthase (CIT2) and aconitase (ACO1) at the transcript and protein levels, glutamate auxotrophy, and changes in the interaction between Rtg2p and the negative regulator Mks1p. Our findings show that all four Rtg2p homologs are functional upon activation of retrograde signaling, although their degree of complementation varied. In addition, all Rtg2p homologs showed a marked reduction in Mks1p binding, which may contribute to their altered responses to retrograde signaling. © 2013 The Authors.en_US
dc.identifier.doi10.1111/1567-1364.12055
dc.identifier.endpage503en_US
dc.identifier.issn1567-1356
dc.identifier.issue5en_US
dc.identifier.pmid23711018en_US
dc.identifier.scopus2-s2.0-84880148385en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage495en_US
dc.identifier.urihttps://doi.org/10.1111/1567-1364.12055
dc.identifier.urihttps://hdl.handle.net/20.500.12491/4865
dc.identifier.volume13en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorÜnlü, Ercan Selçuk
dc.language.isoenen_US
dc.relation.ispartofFEMS Yeast Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCitrate Synthaseen_US
dc.subjectGlutamate Auxotrophyen_US
dc.subjectMitochondria/nuclear Signalingen_US
dc.titleCharacterization of fungal RTG2 genes in retrograde signaling of Saccharomyces cerevisiaeen_US
dc.typeArticleen_US

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