Influence of nutrient media on callus induction, somatic embryogenesis and plant regeneration in selected Turkish crocus species

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dc.authorid0000-0003-0060-259X
dc.authorid0000-0002-9131-4085
dc.authorid0000-0001-6262-2866
dc.contributor.authorVerma, Sandeep Kumar
dc.contributor.authorDas, Ashok Kumar
dc.contributor.authorCingöz, Günce Şahin
dc.contributor.authorUslu, Emel
dc.contributor.authorGürel, Ekrem
dc.date.accessioned2021-06-23T18:51:46Z
dc.date.available2021-06-23T18:51:46Z
dc.date.issued2016
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractCallus induction, somatic embryogenesis and plant regeneration were initiated in selected five species of Turkish crocus using three diffrent explants (leaf, stem and corm) cultured on four different media (MS, GB5, LS and CHE). The highest frequencies of callus induction (100%) and shoot regeneration (70%, with 7.2 shoots/callus) were found in the crocus species Crocus oliveri ssp. Oliveri, using the MS medium containing 5% (w/v) sucrose supplemented with (4 mg/L NAA + 4 mg/L TDZ) and (2 mg/L IAA + 2 mg/L TDZ + 2 mg/L BAP). When the embryogenic calli were transferred into the four nutrient media containing (2 mg/L IAA + 2 mg/L TDZ) and 100 mg/L ABA, these further developed into cotyledonary embryos. Maximum number of somatic embryos (2.9 embryos per leaf explant, with a frequency 46.6%) was obtained in C. oliveri ssp. Oliveri. During subculture using the half strength media, cotyledonary embryos gradually developed into plantlets. © 2016 Published by Elsevier B.V.en_US
dc.description.sponsorshipThe authors thank TUBITAK for partial financial support. We are grateful to the Department of Biology, University of AIBU , for extending the lab facilities.en_US
dc.description.urihttps://reader.elsevier.com/reader/sd/pii/S2215017X16300133?token=8FE38F90DD1916EA61AF96671EC525E1B0141B22C11B6471BD4DE121BF1C2C129234D430A983BF344FDF808E29F4CF39&originRegion=eu-west-1&originCreation=20220727115651
dc.identifier.doi10.1016/j.btre.2016.03.006
dc.identifier.endpage74en_US
dc.identifier.issn2215-017X
dc.identifier.scopus2-s2.0-84962163476en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage66en_US
dc.identifier.urihttps://doi.org/10.1016/j.btre.2016.03.006
dc.identifier.urihttps://hdl.handle.net/20.500.12491/3986
dc.identifier.volume10en_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorCingöz, Günce Şahin
dc.institutionauthorUslu, Emel
dc.institutionauthorGürel, Ekrem
dc.language.isoenen_US
dc.publisherElsevier B.V.en_US
dc.relation.ispartofBiotechnology Reportsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCrocus speciesen_US
dc.subjectOrganogeneisen_US
dc.subjectPlant regenerationen_US
dc.subjectSomatic embryogenesisen_US
dc.titleInfluence of nutrient media on callus induction, somatic embryogenesis and plant regeneration in selected Turkish crocus speciesen_US
dc.typeArticleen_US

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