Protoplast isolation and culture in sugar beet (Beta vulgaris L.)

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dc.authorid0000-0003-2379-5363
dc.authorid0000-0001-6262-2866
dc.authorid0000-0001-9381-9688
dc.contributor.authorGürel, Ekrem
dc.contributor.authorGürel, Songül
dc.contributor.authorKaya, Zeki
dc.date.accessioned2021-06-23T18:54:50Z
dc.date.available2021-06-23T18:54:50Z
dc.date.issued2002
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractIn this study, attempts were made to optimise conditions for protoplast isolation from suspension and mesophyll cells, growth of isolated protoplasts and formation of callus from the cultured protoplasts. The enzyme mixture containing 1.0% cellulase, 0.1% hemicellulase, 1.0% pectinase and 0.1% driselase was found to be the most suitable combination for yield and viability of both suspension-and mesophyll-derived protoplasts from sugar beet. Genotypic variation for plating efficiency was significant; ELK345and M1017 lines producing significantly higher plating efficiency (PE) than other lines. On the other hand, suspension-derived protoplasts of line ELK345 formed more colonies than mesophyll-derived protoplasts of the same line. Micro-callus was obtained from only suspension-derived protoplasts of line ÇBM315. The effects of densities on PE of protoplast cultures were significant -i.e., the highest initial plating density (3 × 105 protoplasts ml-1) produced the highest PE. The differences between the levels of BAP or 2,4-D on colony formation of protoplasts were not significant. However, media containing combinations of BAP or 2,4-D were more efficient for cell colony formation than the control (i.e., hormone-free) medium.en_US
dc.identifier.endpage20en_US
dc.identifier.issn0972-2025
dc.identifier.issue1-2en_US
dc.identifier.scopus2-s2.0-0041425210en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage11en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12491/4592
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-0041425210&partnerID=40&md5=4be914b17b1a267dce97e154abd8aa44
dc.identifier.volume3en_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorGürel, Ekrem
dc.language.isoenen_US
dc.relation.ispartofPlant Cell Biotechnology and Molecular Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMicro Colonyen_US
dc.subjectProtoplast Isolationen_US
dc.subjectSugar Beeten_US
dc.subjectSuspension Cultureen_US
dc.titleProtoplast isolation and culture in sugar beet (Beta vulgaris L.)en_US
dc.typeArticleen_US

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