Investigation of penicillin-induced epilepsy model in female rats with different estrous cycles by ECoG method

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dc.contributor.authorCetinkaya, Ayhan
dc.contributor.authorKara, Sinem
dc.contributor.authorBozat, Bihter
dc.contributor.authorYabalak, Ahmet
dc.date.accessioned2024-09-25T19:57:43Z
dc.date.available2024-09-25T19:57:43Z
dc.date.issued2023
dc.departmentAbant İzzet Baysal Üniversitesien_US
dc.description.abstractAim: Epilepsy is a chronic neurological disease characterized by recurrent seizures resulting from an imbalance in neuronal excitation. It is known that there is a tendency for seizures to cluster in both men and women at certain periods. This study aims to compare electrocorticogram (ECoG) recordings in an experimentally induced epilepsy model using penicillin, conducted at different estrous cycles of female rats. Subjects and Methods: Prior to commencing the epilepsy experiments, vaginal smears were collected from the animals every morning for 1 week, at the same time each day, for cytological examination. After confirming that the estrous cycles (proestrus, estrous stage, metestrus, and diestrus) of the animals were normal and healthy, the experiment was initiated. Additionally, H and E staining was performed on the ovaries of the rats sacrificed at the end of the experiment to validate the estrous cycle phases. A total of 32 adult female Wistar rats were divided into four groups (proestrus, estrus, metestrus, and diestrus), each consisting of eight rats. The penicillin-induced epilepsy model involved anesthetizing the rats with 1.25 mg/kg urethane intraperitoneally, followed by the injection of penicillin G (500 IU, 2.5 mu L, i.c.) into the cortex region through a skull opening. ECoG recordings were conducted using PowerLab analysis for 180 min. Statistical analysis of the results was performed using the SPSS program version 22 (IBM Corp., Armonk, NY, USA). One-way analysis of variance (ANOVA) and independent samples t-test were employed for groups with normally distributed data, while the Kruskal-Wallis test and Mann-Whitney U-test were used for groups with nonnormally distributed data. Statistical significance was set at P < 0.05. Results: Histological evaluation confirmed that vaginal smears and ovarian histopathology were consistent with each other, validating the data pertaining to the groups. In the ECoG recording results, the spike-wave frequency between 6 and 10 min was higher in the metestrus group compared to the proestrus (P = 0.004), estrus (P = 0.046), and diestrus (P = 0.025) groups. No significant differences were observed among the groups in terms of spike-wave amplitude and latency. Conclusion: Our study using a penicillin-induced epilepsy model in female Wistar rats demonstrated that the spike-wave frequency was highest during the metestrus phase. This finding provides valuable insights for future studies aiming to understand and mitigate the sensitivity observed during the metestrus phase, which is characterized by rapid progesterone withdrawal.en_US
dc.identifier.doi10.4103/nsn.nsn_68_23
dc.identifier.endpage187en_US
dc.identifier.issn2636-865X
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-85183546737en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage179en_US
dc.identifier.trdizinid1263287en_US
dc.identifier.urihttps://doi.org/10.4103/nsn.nsn_68_23
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1263287
dc.identifier.urihttps://hdl.handle.net/20.500.12491/13568
dc.identifier.volume40en_US
dc.identifier.wosWOS:001188380500004en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherWolters Kluwer Medknow Publicationsen_US
dc.relation.ispartofNeurological Sciences And Neurophysiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzYK_20240925en_US
dc.subjectElectrocorticographyen_US
dc.subjectepilepsyen_US
dc.subjectestrous cycleen_US
dc.subjectfemale raten_US
dc.subjectpenicillinen_US
dc.titleInvestigation of penicillin-induced epilepsy model in female rats with different estrous cycles by ECoG methoden_US
dc.typeArticleen_US

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