Molecular identification of the yeast strains associated with spontaneous wine fermentation of Kalecik Karası and Emir Grapes

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dc.authorid0000-0003-0438-1130
dc.authorid0000-0003-3158-0901
dc.authorid0000-0002-1189-800X
dc.contributor.authorKarasu-Yalçın, Seda
dc.contributor.authorŞenses-Ergül, Şule
dc.contributor.authorÖzbaş, Z. Yeşim
dc.date.accessioned2021-06-23T19:34:05Z
dc.date.available2021-06-23T19:34:05Z
dc.date.issued2013
dc.departmentBAİBÜ, Mühendislik Fakültesi, Gıda Mühendisliği Bölümüen_US
dc.description.abstractIn this study, 49 yeast isolates, which had been isolated during spontaneous wine fermentation of Kalecik Karasi and Emir grapes, were identified by molecular methods. Nineteen of the 24 Kalecik Karasi isolates and 22 of the 25 Emir isolates could be identified to species level by Restriction Fragment Length Polymorphism (RFLP) of the ITS-18S rRNA gene. The identified Kalecik Karasi isolates were found to be strains of Candida pulcherrima, Candida robusta, Cryptococcus albidus, Kloeckera apiculata, Kloeckera apis, Saccharomyces cerevisiae and Torulaspora delbrueckii. The Emir isolates belonged to Candida krusei, C. pulcherrima, K. apiculata and S. cerevisiae. All of the S. cerevisiae strains and most of the non-Saccharomyces strains could be differentiated by randomly amplified polymorphic DNA (RAPD-PCR) analysis. Although satisfactory results were obtained by M13 primer, (GTG)(3) primer was much more effective in the discrimination of K. apiculata, Cry. albidus and C. krusei strains.en_US
dc.identifier.endpage2672en_US
dc.identifier.issn0973-7510
dc.identifier.issue4en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage2653en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12491/7370
dc.identifier.volume7en_US
dc.identifier.wosWOS:000331428900021en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorKarasu-Yalçın, Seda
dc.language.isoenen_US
dc.publisherDr M N Khanen_US
dc.relation.ispartofJournal Of Pure And Applied Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectYeasten_US
dc.subjectWineen_US
dc.subjectSpontaneous Fermentationen_US
dc.subjectMolecular Identificationen_US
dc.subjectPCR-RFLPen_US
dc.subjectRAPD-PCRen_US
dc.titleMolecular identification of the yeast strains associated with spontaneous wine fermentation of Kalecik Karası and Emir Grapesen_US
dc.typeArticleen_US

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Gıda Mühendisliği Bölümü Koleksiyonu
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