iPBS retrotransposons 'a universal retrotransposons' now in molecular phylogeny of fungal pathogens
Yükleniyor...
Tarih
2016
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Pergamon-Elsevier Science Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Methods for the diagnostics and detection of plant pathogens need to be quick, simple, reliable, and cost effective. In the present study, inter-priming binding sites (iPBS)-retrotransposons were used to study the genetic diversity of 96 isolates belonging to 16 different fungal species at intra- and inter-species levels. The 6 iPBS-retrotransposon primers amplified 389 bands, of which 387 were polymorphic (99%) with an average of 64.83 polymorphic fragments per primer. Polymorphism information contents per primers ranged from 0.976 to 0.870 with the average being 0.972. Neighbor-joining analysis differentiated all fungal isolates according to their species, and at the intra-specific level revealed considerable genetic variability among fungal isolates. The data supports the use of iPBS-retrotransposons as 'a universal marker' for the molecular characterization of different fungal species. It also suggests that only one or a few iPBS-retrotransposon primers would be sufficient for the discrimination of fungal isolates at both intra- and inter-species levels.
Açıklama
Anahtar Kelimeler
iPBS, Retrotransposons, Fungi, Genetic Diversity
Kaynak
Biochemical Systematics And Ecology
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
68
