Evaluation of the analytical performance of two vitamin D immunoassay methods

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dc.authorid0000-0002-7006-3125
dc.authorid0000-0002-4060-3354
dc.contributor.authorYis, Özgür Mehmet
dc.contributor.authorBuğdaycı, Güler
dc.contributor.authorYıldız, Rabia Nur
dc.contributor.authorOğuzman, Hamdi
dc.date.accessioned2021-06-23T18:32:35Z
dc.date.available2021-06-23T18:32:35Z
dc.date.issued2019
dc.departmentBAİBÜ, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractObjectives: The increasing clinical significance of 25-hydroxyvitamin D [25(OH)D] has made it an indispensable test in the laboratory. The aim of this study was to investigate the analytical performance of 2 widely used immunoassay methods, the new Abbott Architect 5P02 25-OH Vitamin D assay (Abbott Laboratories, Lake Bluff, IL, USA) and the Beckman Coulter product, the Access 25 (OH) Vitamin D Total assay (Beckman Coulter, Inc., Brea, CA, USA). Methods: A total of 96 serum samples were used to compare the accuracy, linearity, limit of blank, limit of detection, limit of quantification, and precision of the 2 tests using the Architect i2000SR System (Abbott Laboratories, Lake Bluff, IL, USA) and the Access2 (Beckman Coulter, Brea, CA, USA) immunoassay platform. Deming regression analysis and Bland Altman graphs were used to compare the 2 methods. Results: The within-run coefficient of variation (CV%) of the Architect and the Access systems was <3.1% and <6.72%, respectively. There was good linearity of the 25(OH)D measurement at 98.9-114% for the Architect assay and 94.1- 103.5% for the Access assay at concentrations of 5-160 ng/mL and 6.2-210.4 ng/mL, respectively. The correlation coefficient was 0.921 (y=-2.1+0.97x). Conclusion: Thorough testing indicated that the new 5P02 Abbott Architect 25(OH)D and the Beckman Coulter Access2 25(OH)D Total test demonstrated appropriate analytical performance.en_US
dc.identifier.endpage112en_US
dc.identifier.issn2587-2362
dc.identifier.issn2618-642X
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85145003982en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage107en_US
dc.identifier.trdizinid331820en_US
dc.identifier.urihttps://app.trdizin.gov.tr/makale/TXpNeE9ESXdNQT09
dc.identifier.urihttps://hdl.handle.net/20.500.12491/1935
dc.identifier.volume2en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.institutionauthorYis, Özgür Mehmet
dc.institutionauthorBuğdaycı, Güler
dc.institutionauthorYıldız, Rabia Nur
dc.institutionauthorOğuzman, Hamdi
dc.language.isoenen_US
dc.relation.ispartofInternational Journal of Medical Biochemistryen_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectImmunoassay
dc.subjectMethod Comparison
dc.subjectVitamin D
dc.titleEvaluation of the analytical performance of two vitamin D immunoassay methodsen_US
dc.typeArticleen_US

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