Development, characterization and mapping of microsatellite markers for lentil (Lens culinaris Medik.)

dc.authorid0000-0002-7470-0080
dc.authorid0000-0001-5774-8990
dc.authorid0000-0002-0405-6912
dc.contributor.authorAndeden, Enver Ersoy
dc.contributor.authorBaloch, Faheem Shahzad
dc.contributor.authorÇakır, Esra
dc.contributor.authorToklu, Faruk
dc.contributor.authorÖzkan, Hakan
dc.date.accessioned2021-06-23T18:55:00Z
dc.date.available2021-06-23T18:55:00Z
dc.date.issued2015
dc.departmentBAİBÜ, Ziraat Fakültesi, Tarla Bitkileri Bölümüen_US
dc.description.abstractLentil is the sixth most important pulse crop terms of production in the world, but the number of available and mapped SSR markers are limited. To develop SSR markers in lentil, four genomic libraries for (CA)n, (GA)n, (AAC)n and (ATG)n repeats were constructed. A total of 360 SSR primers were designed and validated using 15 Turkish lentil cultivars and genotypes. The most polymorphic repeat motifs were GA and CT, with a mean number of alleles per locus of 7.80 and 6.55, respectively. Seventy-eight SSR primers amplified a total of 400 polymorphic alleles, whereas 71 SSR primers produced markers within the expected size range. For 78 polymorphic SSR primers, the average number of alleles per locus was 5.1 and PIC value ranged from 0.07 to 0.89, with an average of 0.58. A linkage map was constructed using 92 individual F2 plants derived from a cross between Karacadağ × Silvan, with 47 SSR markers. The SSR markers developed in this study could be used for germplasm classification and identification and mapping of QTL in lentil. © 2015 Blackwell Verlag GmbH.en_US
dc.identifier.doi10.1111/pbr.12296
dc.identifier.endpage598en_US
dc.identifier.issn0179-9541
dc.identifier.issue5en_US
dc.identifier.scopus2-s2.0-84942992183en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage589en_US
dc.identifier.urihttps://doi.org/10.1111/pbr.12296
dc.identifier.urihttps://hdl.handle.net/20.500.12491/4650
dc.identifier.volume134en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorBaloch, Faheem Shahzad
dc.language.isoenen_US
dc.publisherBlackwell Publishing Ltden_US
dc.relation.ispartofPlant Breedingen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEnriched Genomic Librariesen_US
dc.subjectGenetic dDversityen_US
dc.subjectLentilen_US
dc.subjectLinkage Mappingen_US
dc.subjectSimple Sequence Repeatsen_US
dc.titleDevelopment, characterization and mapping of microsatellite markers for lentil (Lens culinaris Medik.)en_US
dc.typeArticleen_US

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