Poly(vinyl alcohol)/polyethyleneimine (PVA/PEI) blended monolithic cryogel columns for the depletion of haemoglobin from human blood
Küçük Resim Yok
Tarih
2016
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
We have synthesized PVA/PEI monolithic cryogel columns chelated with Cu2+ ions as a model adsorbent, which is capable of binding haemoglobin (Hb) from human blood. The goal of this study is to perform the depletion of Hb via a single and easy process to be useful in proteomic studies. PVA/PEI-Cu2+ cryogel columns were subjected to adsorption studies of Hb from both aqueous solution and human plasma to evaluate the extent of interaction between cryogel columns and Hb. The effects of experimental parameters, such as pH, Hb equilibrium concentration, adsorption time, temperature, and ionic strength, on Hb adsorption capacity were investigated.
Açıklama
Anahtar Kelimeler
Cryogel, haemoglobin, IMAC, PEI-Cu2+, PVA
Kaynak
Separation Science And Technology
WoS Q Değeri
Q3
Scopus Q Değeri
Cilt
51
Sayı
11
