Neurotoxicity evaluation of three root canal sealers on cultured rat trigeminal ganglion neurons

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dc.authorid0000-0002-0667-4909
dc.authorid0000-0002-9449-9564
dc.authorid0000-0002-7574-1183
dc.authorid0000-0001-8512-5280
dc.authorid0000-0002-1951-3987
dc.contributor.authorEr, Kürşat
dc.contributor.authorAyar, Ahmet
dc.contributor.authorKalkan, Ömer Faruk
dc.contributor.authorTaşdemir, Tamer
dc.contributor.authorCanpolat, Sinan
dc.contributor.authorOzan, Ülkü
dc.date.accessioned2021-06-23T18:51:42Z
dc.date.available2021-06-23T18:51:42Z
dc.date.issued2017
dc.departmentBAİBÜ, Diş Hekimliği Fakültesi, Endodonti Ana Bilim Dalıen_US
dc.description.abstractBackground: The aim of this study was to investigate the possible neurotoxic effects of 3 root canal sealers (RCSs) (AH Plus, GuttaFlow, iRoot SP) on cultured rat trigeminal ganglion (TG) neurons. Material and Methods: Primary cultures of TG neurons were obtained from 1 to 2-day old rats. Freshly mixed RCSs were incubated in sterile phosphate buffered saline and cells were incubated with supernatants of the RCSs for different time intervals (1-, 3-, 6- and 24-h; 1 or 1/10 diluted) and viability/cytotoxicity was tested by counting the number of live cells. Pair of dishes with cells from the same culture incubated with only culture medium was considered as negative controls. Cell images were captured and acquired at x200 magnification using a microscope equipped with a camera using special image program. The viable cells were manually counted assigned from the images for each dose and incubation duration. Data was analysed by using 1-way analysis of variance with Tukey post hoc tests. Results: There was no significant change in cell viability after short duration of incubation (1- and 3-h) with the supernatant of any of RCSs, except for undiluted-AH Plus at 3-h. When AH Plus was compared with other RCSs, for diluted supernatants, there was only significant difference between iRoot SP and AH Plus at 24-h (P < 0.05). Whereas undiluted-AH Plus was significantly more cytotoxic for 3-, 6- and 24-h periods as compared to respective incubation periods of undiluted other groups (P < 0.05). GuttaFlow groups had similar neurotoxic effect on cells for all test periods. Conclusions: All tested RCSs exhibited a variable degree of neurotoxicity on these primary sensory neurons of orofacial tissues, depending on their chemical compositions. GuttaFlow and iRoot SP evoked a less toxic response to TG cells than AH Plus. © Medicina Oral S. L. C.I.F. B 96689336.en_US
dc.identifier.doi10.4317/jced.52901
dc.identifier.endpagee39en_US
dc.identifier.issn1989-5488
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85011928517en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpagee34en_US
dc.identifier.urihttps://doi.org/10.4317/jced.52901
dc.identifier.urihttps://hdl.handle.net/20.500.12491/3953
dc.identifier.volume9en_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorOzan, Ülkü
dc.language.isoenen_US
dc.publisherMedicina Oral S.L.en_US
dc.relation.ispartofJournal of Clinical and Experimental Dentistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAH Plusen_US
dc.subjectCell cultureen_US
dc.subjectGuttaFlowen_US
dc.subjectIRoot SPen_US
dc.subjectNeurotoxicityen_US
dc.subjectRoot canal sealeren_US
dc.subjectTrigeminal gangliaen_US
dc.titleNeurotoxicity evaluation of three root canal sealers on cultured rat trigeminal ganglion neuronsen_US
dc.typeArticleen_US

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