Inter-primer binding site retrotransposon and inter-simple sequence repeat diversity among wild Lens species

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dc.authorid0000-0002-7470-0080en_US
dc.authorid0000-0001-7078-3221en_US
dc.authorid0000-0001-5774-8990en_US
dc.authorid0000-0002-9145-6980en_US
dc.authorid0000-0003-3530-2626en_US
dc.authorid0000-0002-6522-4596en_US
dc.contributor.authorBaloch, Faheem Shehzad
dc.contributor.authorDerya, Muazzez
dc.contributor.authorAndeden, Enver Ersoy
dc.contributor.authorAlsaleh, Ahmad
dc.contributor.authorCömertpay, Gönül
dc.contributor.authorKilian, Benjamin
dc.contributor.authorÖzkan, Hakan
dc.date.accessioned2021-06-23T19:42:20Z
dc.date.available2021-06-23T19:42:20Z
dc.date.issued2015
dc.departmentBAİBÜ, Ziraat Fakültesi, Tarla Bitkileri Bölümüen_US
dc.description.abstractEven though lentil has been an important food legume for centuries, genetic studies in lentil are still in their infancy. Genetic diversity and relationships among wild Lens species from Turkey has seldom been investigated. Additionally, a limited number of simple sequence repeat (SSR) markers have been developed for use in breeding and genetic studies of lentil crop. In this study, molecular characterization of 50 accessions mostly from Turkey, belonging to 6 wild and 1 cultivated Lens species, was performed using newly developed inter-primer binding site (iPBS) retrotransposons and inter-SSR (ISSR) markers. The 10 iPBS primers generated a total of 151 scorable bands, of which 150 were polymorphic (99.3%) with an average of 15.0 polymorphic fragments per primer. The 10 ISSR primers detected 138 scorable bands showing 100% polymorphism, with an average of 13.5 bands per primer. The average polymorphism information content (PIC) value for ISSR markers (0.97) was higher than that for iPBS markers (0.90). Lens orientalis was found to be the most diverse species, raising the possibility of wide crosses with cultivated species Lens culinaris. Cultivated varieties also showed high level of polymorphism, at 82.92% and 51.92% with ISSR and iPBS markers, respectively. Lens lamottei and Lens tomentosus were found as the least polymorphic species using both marker systems. The grouping of accessions and species within clusters were almost similar when iPBS and ISSR graphs were compared. Our data also suggested the role of iPBS-retrotransposons as 'a universal marker' for molecular characterization of wild and cultivated Lens species. (C) 2014 Elsevier Ltd. All rights reserved.en_US
dc.identifier.doi10.1016/j.bse.2014.12.002
dc.identifier.endpage168en_US
dc.identifier.issn0305-1978
dc.identifier.issn1873-2925
dc.identifier.scopus2-s2.0-84920140057en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage162en_US
dc.identifier.urihttps://doi.org/10.1016/j.bse.2014.12.002
dc.identifier.urihttps://hdl.handle.net/20.500.12491/8445
dc.identifier.volume58en_US
dc.identifier.wosWOS:000351320000025en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorBaloch, Faheem Shehzad
dc.language.isoenen_US
dc.publisherPergamon-Elsevier Science Ltden_US
dc.relation.ispartofBiochemical Systematics And Ecologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectRetrotransposonsen_US
dc.subjectWild Speciesen_US
dc.subjectLentilen_US
dc.subjectGenetic Resourcesen_US
dc.subjectTaxonomyen_US
dc.subjectTurkeyen_US
dc.titleInter-primer binding site retrotransposon and inter-simple sequence repeat diversity among wild Lens speciesen_US
dc.typeArticleen_US

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