Molecular identification of some yeast strains isolated from various sugary foods

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dc.authorid0000-0003-0438-1130
dc.authorid0000-0002-1189-800_
dc.contributor.authorŞenses-Ergül, Şule
dc.contributor.authorKarasu-Yalçın, Seda
dc.contributor.authorÖzbaş, Z. Yeşim
dc.date.accessioned2021-06-23T19:28:53Z
dc.date.available2021-06-23T19:28:53Z
dc.date.issued2012
dc.departmentBAİBÜ, Mühendislik Fakültesi, Gıda Mühendisliği Bölümüen_US
dc.description.abstractIn this study, 37 yeast strains belonging to 19 different species, which had been isolated from various Turkish sugary foods, were characterized by molecular methods. Identification of yeast isolates at the species level was performed with restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) by using five different restriction enzymes (MspI, HaeIII, AluI, RsaI, and ScrFI). For each enzyme, different restriction patterns obtained for endogenic yeast strains were compared to those obtained for the control (type or reference) strains. The strains could be identified when their digestion profiles obtained by all of the enzymes matched those of the control strains. Among the yeast strains, 16 could be identified adequately by RFLP-PCR. Tested yeast species were determined as Candida colliculosa, Candida guilliermondii, Candida kefyr, Candida lambica, Candida parapsilosis, Candida pelliculosa, Candida pulcherrima, Pichia anomala, Saccharomyces cerevisiae, Torulaspora delbrueckii and Zygosaccharomyces rouxii. Strain variation was determined by randomly amplified polymorphic DNA (RAPD-PCR) analysis by using (GTG)(3) and M13 primers. The discrimination among the strains were detected by cluster analysis. Generally, strains of the same species clustered together with a few exceptions. By this way, all the strains in the same species could be differentiated by using both the primers.en_US
dc.identifier.doi10.1007/s13213-011-0404-5
dc.identifier.endpage1516en_US
dc.identifier.issn1590-4261
dc.identifier.issn1869-2044
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-84871446328en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1501en_US
dc.identifier.urihttps://doi.org/10.1007/s13213-011-0404-5
dc.identifier.urihttps://hdl.handle.net/20.500.12491/7081
dc.identifier.volume62en_US
dc.identifier.wosWOS:000310886200020en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorKarasu-Yalçın, Seda
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofAnnals Of Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectYeasten_US
dc.subjectHigh-sugar Fooden_US
dc.subjectMolecular Identificationen_US
dc.subjectRFLP-PCRen_US
dc.subjectRAPD-PCRen_US
dc.titleMolecular identification of some yeast strains isolated from various sugary foodsen_US
dc.typeArticleen_US

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