The impact of sucrose and 6-benzylaminopurine on shoot propagation and vitrification in Aroniamelanocarpa (black chokeberry)

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dc.contributor.authorBayhan, Nida
dc.contributor.authorYucesan, Buhara
dc.date.accessioned2024-09-25T19:56:22Z
dc.date.available2024-09-25T19:56:22Z
dc.date.issued2024
dc.departmentAbant İzzet Baysal Üniversitesien_US
dc.description.abstractVitrification is one of the most significant issues encountered in plant tissue culture applications. It diminishes the quality of in vitro plants, causing their leaves and stems to appear watery and translucent, and it may impede the success of the acclimatization step. In this respect, this study investigates the impact of sucrose and 6-Benzylaminopurine (BAP) concentrations on shoot regeneration and vitrification development in Aronia melanocarpa, known for its high antioxidant content and health benefits. Initially, the presence of BAP, in combination with varying sucrose concentrations, leads to a substantial increase in shoot number, and the largest number (7 shoots per nodal explant) was observed in the Murashige and Skoog (MS) medium containing 3% sucrose and 5.0 mg/L BAP. Furthermore, sucrose concentration plays a crucial role in shoot growth, with higher concentrations promoting more extensive shoot development. However, when 3% sucrose was combined with higher BAP (from 1.0- to 5.0 mg/L), an increased incidence of vitrification was observed over time. Interestingly, lower sucrose concentrations (1% or 2%) combined with 0.5 mg/L or 2.5 mg/L BAP initially delayed vitrification but eventually led to its occurrence. Microscopic analysis of leaf samples with varying levels of vitrification indicates significant differences in the density of stomata, further confirming the detrimental impact of vitrification on cellular structures and physiological processes. The recovery of vitrified plants was evaluated using different growth media combinations. The absence of BAP in the medium led to higher recovery percentages (min 96%) without necrosis, while the addition of 0.5 mg/L BAP promoted shoot growth but potentially inhibited root development. It has been found that media with 1 mg/L BAP and either 10 g/L or 20 g/L sucrose, as well as media with 30 g/L sucrose and 0.5 mg/L BAP, are the most suitable for efficient shoot regeneration with minimal vitrification risk. However, increasing BAP levels for faster shoot regeneration also raises the risk of vitrification. During acclimatization, vitrified plants exhibited stunted shoot growth, shorter and narrower leaves, reduced root numbers and lengths, and decreased survival rates, particularly under lower humidity conditions. The cultivation period required for the recovery of the crop was determined to be 6 weeks under greenhouse conditions for a sustainable plant propagation.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkiye (TUBiTAK); Scientific Council of Bolu Abant Izzet Baysal University [2022.10.04.1558]en_US
dc.description.sponsorshipOpen access funding provided by the Scientific and Technological Research Council of Turkiye (TUBiTAK). This study was financially supported by the Scientific Council of Bolu Abant Izzet Baysal University under the project number 2022.10.04.1558.en_US
dc.identifier.doi10.1007/s11240-023-02652-x
dc.identifier.issn0167-6857
dc.identifier.issn1573-5044
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85182403314en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1007/s11240-023-02652-x
dc.identifier.urihttps://hdl.handle.net/20.500.12491/13254
dc.identifier.volume156en_US
dc.identifier.wosWOS:001143284400001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofPlant Cell Tissue And Organ Cultureen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzYK_20240925en_US
dc.subjectAronia melanocarpaen_US
dc.subjectBlack chokeberryen_US
dc.subjectVitrificationen_US
dc.subjectShoot regenerationen_US
dc.subject6-benzylaminopurine (BAP)en_US
dc.subjectSucroseen_US
dc.titleThe impact of sucrose and 6-benzylaminopurine on shoot propagation and vitrification in Aroniamelanocarpa (black chokeberry)en_US
dc.typeArticleen_US

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