Selective dopamine detection by SPR sensor signal amplification using gold nanoparticles

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dc.authorid0000-0003-0161-172Xen_US
dc.authorid0000-0002-5737-720Xen_US
dc.authorid0000-0001-7292-7241en_US
dc.authorid0000-0002-6672-6862en_US
dc.authorid0000-0003-3260-1639en_US
dc.contributor.authorTürkmen, Deniz
dc.contributor.authorBakhshpour, Monireh
dc.contributor.authorGöktürk, Ilgım
dc.contributor.authorAşır, Süleyman
dc.contributor.authorYılmaz, Fatma
dc.date.accessioned2023-06-14T08:07:43Z
dc.date.available2023-06-14T08:07:43Z
dc.date.issued2021en_US
dc.departmentBAİBÜ, Gerede Meslek Yüksekokulu, Kimya ve Kimyasal İşleme Teknolojileri Bölümüen_US
dc.description.abstractIn this study, selective and sensitive detection of the neurotransmitter dopamine (DA) in both aqueous solution and biological samples was performed using a surface plasmon resonance (SPR) sensor based on a molecular imprinting technique. For this, dopamine-imprinted poly(2-hydroxyethyl methacrylate-N-methacroyl-(l)-cysteine methyl ester-gold nanoparticles-N-methacryloyl-l-phenylalanine methyl ester) [PHEMAC-AuNPs/MAPADA] nanoparticles were prepared. Furthermore, to evaluate the imprinting efficiency, non-imprinted [PHEMAC-AuNPs/MAPA] nanoparticles were designed using the same polymerization procedure except for the addition of dopamine molecules. To examine the effect of incorporating AuNPs to increase the SPR signal response, control experiments were carried out via the SPR biosensor produced using [PHEMAC/MAPADA] nanoparticles prepared without the addition of AuNPs. Characterization studies of dopamine- + imprinted [PHEMAC-AuNPs/MAPADA] and non-imprinted [PHEMAC-AuNPs/MAPA] nanoparticles was performed with a zetasizer and an FTIR-ATR spectrophotometer. In addition, dopamine-imprinted [PHEMAC-AuNPs/MAPADA] and non-imprinted [PHEMAC-AuNPs/MAPADA] SPR sensors were characterized by ellipsometer and contact angle measurements. The high imprinting efficiency (I.F: 9.67) of the dopamine-imprinted [PHEMAC-AuNPs/MAPADA] SPR sensor was determined by comparing it with the non-imprinted [PHEMAC-AuNPs/MAPA] SPR sensor. A good linear relationship was obtained in the 0.01-0.5 ppb concentration range with correlation coefficients of 0.9818 and 0.9819, respectively. The dopamine-imprinted [PHEMAC-AuNPs/MAPADA] SPR sensor was 5.53 and 4.59 times more selective for the target molecule dopamine than for epinephrine (EP) and norepinephrine (NE), respectively. The repeatability of the [PHEMAC-AuNPs/MAPADA] SPR sensor was assessed with a 0.5 ppb dopamine solution, with the percent relative standard deviation of the intra-assays (RSD) being less than 1.7%, indicating negligible loss of dopamine sensing capability after four adsorption-desorption cycles with the same sensor.en_US
dc.identifier.citationTürkmen, D., Bakhshpour, M., Göktürk, I., Aşır, S., Yılmaz, F., & Denizli, A. (2021). Selective dopamine detection by SPR sensor signal amplification using gold nanoparticles. New Journal of Chemistry, 45(39), 18296-18306.en_US
dc.identifier.doi10.1039/d1nj01938d
dc.identifier.endpage18306en_US
dc.identifier.issn1144-0546
dc.identifier.issn1369-9261
dc.identifier.issue36en_US
dc.identifier.scopus2-s2.0-85117384573en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage18296en_US
dc.identifier.urihttp://dx.doi.org/10.1039/d1nj01938d
dc.identifier.urihttps://hdl.handle.net/20.500.12491/11114
dc.identifier.volume45en_US
dc.identifier.wosWOS:000697256200001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorYılmaz, Fatma
dc.language.isoenen_US
dc.publisherROYAL SOC CHEMISTRYen_US
dc.relation.ispartofNew Journal of Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHighly Sensitive Detectionen_US
dc.subjectSilver Nanoparticlesen_US
dc.subjectImprinted Polymeren_US
dc.subjectGraphene Oxideen_US
dc.subjectBiosensoren_US
dc.titleSelective dopamine detection by SPR sensor signal amplification using gold nanoparticlesen_US
dc.typeArticleen_US

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