Purification and in vitro toxicity of gamma amanitin

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dc.authorid0000-0003-1170-6036
dc.authorid0000-0001-7550-6370
dc.contributor.authorBakırcı, Sinan
dc.contributor.authorBayram, Recep
dc.contributor.authorYılmaz, İsmail
dc.contributor.authorYaykaşlı, Kürşat Oğuz
dc.contributor.authorBayram, Sait
dc.date.accessioned2021-06-23T19:42:25Z
dc.date.available2021-06-23T19:42:25Z
dc.date.issued2015
dc.departmentBAİBÜ, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümüen_US
dc.description.abstractWe aimed to obtain gamma amanitin with high purity through a purification process and compare toxic effects of alpha, beta, and gamma amanitin. Specific concentrations of the toxins (25, 10, 1, and 0.1 mu g/mL) were applied to the C3A human hepatocytes. A MTT test was performed to determine the level of toxicity. Alpha amanitin showed a higher toxicity in 48 h while the lowest toxicity was observed in beta amanitin. The toxicity level of gamma amanitin was found between the alpha and beta amanitin toxicity. Our method is suitable for obtaining gamma amanitin with high purity (>99%) as well as for obtaining alpha amanitin and beta amanitin. Gamma amanitin has been shown to have equal responsibility for toxicity as alpha amanitin in amanita poisoning.en_US
dc.identifier.doi10.3109/15569543.2015.1135172
dc.identifier.endpage205en_US
dc.identifier.issn1556-9543
dc.identifier.issn1556-9551
dc.identifier.issue4en_US
dc.identifier.startpage200en_US
dc.identifier.urihttps://doi.org/10.3109/15569543.2015.1135172
dc.identifier.urihttps://hdl.handle.net/20.500.12491/8479
dc.identifier.volume34en_US
dc.identifier.wosWOS:000375245500008en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.institutionauthorBayram, Recep
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofToxin Reviewsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAlpha Amanitinen_US
dc.subjectBeta Amanitinen_US
dc.subjectGamma Amanitinen_US
dc.subjectMTT Assayen_US
dc.subjectC3A Human Hepatocyte Cell Lineen_US
dc.titlePurification and in vitro toxicity of gamma amanitinen_US
dc.typeArticleen_US

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