Nitrite oxidation and tyrosine nitration by human myeloperoxidase

dc.authorid0000-0002-2948-2635
dc.authorid0000-0002-0310-9998
dc.contributor.authorSarkarati, Bahram
dc.contributor.authorKılınç, Kamer
dc.date.accessioned2021-06-23T19:43:02Z
dc.date.available2021-06-23T19:43:02Z
dc.date.issued2016
dc.departmentBAİBÜ, Rektörlük, Bilimsel Endüstriyel ve Teknolojik Uygulama ve Araştırma Merkezien_US
dc.description41st FEBS Congress on Molecular and Systems Biology for a Better Life -- SEP 03-08, 2016 -- Kusadasi, TURKEYen_US
dc.description.abstractBiomineralization is deposition of hydroxyapatite, the crystallized mineral form of calcium and phosphate, by cells to their extracellular matrix (ECM), and it is an essential mechanism of bone and teeth formation in humans. Biomineralization is especially important in adults for tissue regeneration in bone defects. ECM molecules regulate mineral formation and provide crystal growth and nucleation. One of the most important ECM molecules, Alkaline Phosphatase (ALP), is the key enzyme in biomineralization process by the activity of converting organophosphate into inorganic phosphate. Moreover, osteocalcin and osteopontin are small soluble noncollagenous proteins of ECM and they regulate biomineralization by binding to calcium atoms available at crystal surfaces due to their highly negative charged amino acid residues. In this study, ALP, osteocalcin and osteopontin are expressed in bacterial systems and purified to assess in vitro biomineralization. Optimization of in vitro biomineralization activities with osteocalcin and osteopontin proteins provided the understanding of the effect of protein concentrations in crystal structure of calcium crystals. Understanding of the effect of protein concentrations will provide control over biomineralization in different cell types by designing synthetic genetic circuits. Programming non-biomineral formation cells for biomineral formation will enable differentiation free bone mineral formation. Reprogramming of non-biomineral formation cells will help to treat bone defects and bone-impairing diseases, such as osteoporosis. Consequently, it is an outstanding approach to understand the activity of bone ECM proteins and construct synthetic genetic systems that can reprogram non-mineral formation cells for biomineralization within the scope of bone tissue engineering.en_US
dc.description.sponsorshipFEBSen_US
dc.identifier.endpage65en_US
dc.identifier.issn1742-464X
dc.identifier.issn1742-4658
dc.identifier.startpage65en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12491/8675
dc.identifier.urihttps://www.webofscience.com/wos/woscc/full-record/WOS:000383616900193
dc.identifier.volume283en_US
dc.identifier.wosWOS:000383616900193en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.institutionauthorSarkarati, Bahram
dc.language.isoenen_US
dc.publisherWiley-Blackwellen_US
dc.relation.ispartofFebs Journalen_US
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectNitrite Oxidationen_US
dc.subjectTyrosine Nitrationen_US
dc.subjectMyeloperoxidaseen_US
dc.subjectNO2-en_US
dc.subjectNO2en_US
dc.titleNitrite oxidation and tyrosine nitration by human myeloperoxidaseen_US
dc.typeConference Objecten_US

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