Characterisation of Cryphonectria hypovirus 1 strains in Turkey and their transmission to various vegetative compatibility types of Cryphonectria parasitica

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dc.contributor.authorCakar, Deniz
dc.contributor.authorOzer, Goksel
dc.contributor.authorSimsek, Secil Akilli
dc.contributor.authorMaden, Salih
dc.date.accessioned2024-09-25T19:57:25Z
dc.date.available2024-09-25T19:57:25Z
dc.date.issued2024
dc.departmentAbant İzzet Baysal Üniversitesien_US
dc.description.abstractCharacterization of the Cryphonectria parasitica population was initially done by a phenotypical assessment of 40 in vitro grown isolates obtained from 52 healing cankers collected from eight important chestnut-growing provinces of Turkey. The results of Bavendamm test, often correlated indirectly to hypovirulence, suggested 31 possibly hypovirulent and 9 virulent isolates. PCR tests amplified two regions of ORFs A and B of Cryphonectria hypovirus 1 (CHV-1) from 36 of 40 isolates. The PCR test confirmation was more sensitive than the Bavendamm test. Partial ORFA sequencing revealed 36 CHV-1 haplotypes belonging to Italian subtype (I), with all hypovirulent isolates being of EU-1 vc type. The CHV-1 from 10 native EU-1 isolates were first transferred to six European vc type testers, EU-2, EU-3, EU-5, EU-7, EU-26, and EU-44, having heteroallelism at one vic locus. The presence of the vic locus difference generally reduces virus transmission. The easiest and highest frequency virus transfer was obtained by vic4 and vic6 allelic differences, while the differences vic2 and vic7 made the transfer more challenging. Finally, in this study we successfully transferred CHV-1 to an EU-1 isolate obtained from the Bursa province to an EU-12 European tester isolate.en_US
dc.description.sponsorshipCankimath;rimath; Karatekin University Research Fund [FF210621D05]; Scientific and Technological Re-search Council of Turkiye (TUBIdot;TAK)en_US
dc.description.sponsorshipThis work was supported by Cank & imath;r & imath; Karatekin University Research Fund (Project no: FF210621D05). Open access funding provided by the Scientific and Technological Re-search Council of Turkiye (TUB & Idot;TAK).en_US
dc.identifier.doi10.1007/s42161-024-01691-3
dc.identifier.issn1125-4653
dc.identifier.issn2239-7264
dc.identifier.scopus2-s2.0-85195884938en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1007/s42161-024-01691-3
dc.identifier.urihttps://hdl.handle.net/20.500.12491/13402
dc.identifier.wosWOS:001247207600001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofJournal of Plant Pathologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzYK_20240925en_US
dc.subjectChestnut blighten_US
dc.subjectCastanea sativaen_US
dc.subjectHypovirulenceen_US
dc.subjectHeteroallelismen_US
dc.subjectVegetative incompatibilityen_US
dc.titleCharacterisation of Cryphonectria hypovirus 1 strains in Turkey and their transmission to various vegetative compatibility types of Cryphonectria parasiticaen_US
dc.typeArticleen_US

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