Plasmonic sensing platform for C-reactive protein recognition via synthetic receptors decorated on graphene oxide and gold nanoparticles

Basit Öğe Kaydı
dc.contributor.authorOvezova, Mamajan
dc.contributor.authorYilmaz, Fatma
dc.contributor.authorGokturk, Ilgim
dc.contributor.authorDenizli, Adil
dc.date.accessioned2024-09-25T19:57:35Z
dc.date.available2024-09-25T19:57:35Z
dc.date.issued2024
dc.departmentAbant İzzet Baysal Üniversitesien_US
dc.description.abstractC-reactive protein (CRP) level provides important information about the health status of the individual in predicting many diseases such as cardiovascular, chronic inflammatory, and neurodegenerative diseases. Therefore, determining CRP levels is important for correct health intervention and treatment follow-up. For the selective detection of CRP, we developed a (GO/Au-MIP) SPR sensor containing CRP-imprinted polymer modified with graphene oxide and gold nanoparticles. To prove the enhanced sensitivity of the sensor resulting from the presence of graphene oxide (GO) and gold nanoparticles (AuNPs), a CRP-imprinted (MIP) SPR sensor was prepared using the same method, excluding the incorporation of GO and AuNPs. In addition, a non-imprinted GO/Au-NIP SPR sensor was also prepared to evaluate the imprinting efficiency. In detecting CRP in PBS buffer, the GO/Au-MIP SPR sensor exhibited linearity in the concentration ranges of 0.1-2 ppm (R-2 = 0.9721) and 5-100 ppm (R-2 = 0.9740). The detection limit of the prepared sensor was calculated as 0.0082 ppm. In the study, the imprinting process efficiency was also evaluated by calculating the imprinting factor value (I.F=13.84). In the selectivity studies, the GO/Au-MIP SPR sensor was determined to be 9.23 times more selective against CRP protein than bovine serum albumin and 29.53 times more selective than hemoglobin. When the repeatability of the GO/Au-MIP SPR sensor was examined, it was determined that the GO/Au-MIP SPR sensor was able to detect CRP without any deterioration in performance in five consecutive reuses (RSD<1.5). Finally, CRP detection studies from the serum and urine solutions, which were selected as real samples, were carried out by the GO/Au-MIP SPR sensor to evaluate the matrix effect. CRP spiked serum sample was analyzed by the other standard method to validate the analytical results using CRP analyser.en_US
dc.description.sponsorshipThis article is part of the results of Mamajan Ovezova's Ph.D. thesis submitted to Hacettepe University, Department of Chemistry, Institute of Science, Beytepe, Ankara, Turkey. M. O. thanks the Turkiye Schol-arships for the fellowship.en_US
dc.identifier.doi10.1016/j.microc.2024.111545
dc.identifier.issn0026-265X
dc.identifier.issn1095-9149
dc.identifier.scopus2-s2.0-85203009726en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.microc.2024.111545
dc.identifier.urihttps://hdl.handle.net/20.500.12491/13472
dc.identifier.volume206en_US
dc.identifier.wosWOS:001310844400001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofMicrochemical Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzYK_20240925en_US
dc.subjectC-reactive protein detectionen_US
dc.subjectGraphene oxideen_US
dc.subjectGold nanoparticlesen_US
dc.subjectMolecular imprinted polymeren_US
dc.subjectSurface plasmon resonance sensoren_US
dc.titlePlasmonic sensing platform for C-reactive protein recognition via synthetic receptors decorated on graphene oxide and gold nanoparticlesen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu