Characterization of cereal cyst nematodes in wheat using morphometrics, SCAR markers, RFLP, and rDNA-ITS sequence analyses

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dc.authorid0000-0002-6982-5874en_US
dc.authorid0000-0002-7217-9092en_US
dc.authorid0000-0002-3385-2520en_US
dc.authorid0000-0003-0299-6094en_US
dc.contributor.authorDağlı, Deniz
dc.contributor.authorDuman, Nagihan
dc.contributor.authorYüksel, Ebubekir
dc.contributor.authorYıldız, Şenol
dc.contributor.authorÖzer, Göksel
dc.contributor.authorDababat, Abdelfattah
dc.contributor.authorİmren,Mustafa
dc.date.accessioned2023-08-11T10:21:15Z
dc.date.available2023-08-11T10:21:15Z
dc.date.issued2023en_US
dc.departmentBAİBÜ, Ziraat Fakültesi, Bitki Koruma Bölümüen_US
dc.description.abstractIn this study, morphological and molecular characterizations of twenty-four Heterodera populations (cereal cyst nematodes, CCNs) collected from wheat production fields in Turkey were carried out. Light microscopy, species-specific markers, RFLP, and ITS sequencing were used to identify the nematode populations. The obtained CCN populations were identified as Heterodera avenae, H. filipjevi, and H. latipons according to the morphometric analysis, which was confirmed by the molecular techniques. The ITS region sequencing analysis confirmed the species identification, and phylogenetic analysis of this region grouped the populations with representative Heterodera populations from different origin countries deposited in GenBank. The simulation of four restriction enzymes, Alul, PstI, BsuRI (HaeIII), and Rsal, employed the ITS sequences of isolates to discriminate the Turkish Heterodera populations. ITS-RFLP patterns produced by endonuclease enzymes provided variations among Heterodera species. There was no intraspecific variation in populations of each Heterodera species in the ITS-RFLP analyses. The species-specific primers, AvenF-COI/AvenR-COI, HfF/HfR, and H-LatF/H-LatR, yielded 109 bp, 646 bp, and 204 bp products for H. avenae, H. filipjevi, and H. latipons populations, respectively. This is the first research to provide conclusive diagnostic tests for cyst nematode populations isolated from Turkey. These assays provide a sensitive, practical, and quick method for detecting Heterodera species and, therefore, have the potential to be utilized in the early identification of populations and monitoring of infestations without morphometric studies.en_US
dc.identifier.citationDağlı, D., Duman, N., Yüksel, E., Yıldız, Ş., Özer, G., Dababat, A., & Imren, M. (2023). Characterization of cereal cyst nematodes in wheat using morphometrics, SCAR markers, RFLP, and rDNA-ITS sequence analyses. Tropical Plant Pathology, 48(2), 207-216.en_US
dc.identifier.doi10.1007/s40858-022-00528-7
dc.identifier.endpage216en_US
dc.identifier.issn1983-2052
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85148420874en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage207en_US
dc.identifier.urihttp://dx.doi.org/10.1007/s40858-022-00528-7
dc.identifier.urihttps://hdl.handle.net/20.500.12491/11497
dc.identifier.volume48en_US
dc.identifier.wosWOS:000936088900001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorDağlı, Deniz
dc.institutionauthorDuman, Nagihan
dc.institutionauthorYıldız, Şenol
dc.institutionauthorÖzer, Göksel
dc.institutionauthorİmren, Mustafa
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofTropical Plant Pathologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - İdari Personel ve Öğrencien_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDiagnosticsen_US
dc.subjectHeterodera Avenae Groupen_US
dc.subjectMolecularen_US
dc.subjectMorphometricsen_US
dc.titleCharacterization of cereal cyst nematodes in wheat using morphometrics, SCAR markers, RFLP, and rDNA-ITS sequence analysesen_US
dc.typeArticleen_US

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