Improvement of pectinase production by co-culture of Bacillus spp. using apple pomace as a carbon source

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dc.authorid0000-0003-1050-8206en_US
dc.authorid0000-0002-0537-9860en_US
dc.contributor.authorKuvvet, Ceren
dc.contributor.authorUzuner, Sibel
dc.contributor.authorÇekmecelioğlu, Deniz
dc.date.accessioned2021-06-23T19:51:45Z
dc.date.available2021-06-23T19:51:45Z
dc.date.issued2019
dc.departmentBAİBÜ, Mühendislik Fakültesi, Gıda Mühendisliği Bölümüen_US
dc.description.abstractIncreasing interest in using food and agricultural wastes for low cost enzyme production continues in fermentation industry. Bacteria, especially Bacillus subtilis, are known to produce pectinases in synthetic media and media amended with agricultural wastes as a carbon source, whereas the potential of co-culturing Bacillus species to produce pectinases has not been fully studied yet. In this study, co-culture of B. subtilis and B. pumilus was optimized for solid load and pH to maximize pectinase production in submerged fermentation using apple pomace as the carbon source. Optimal pectinase activity was achieved as 11.25IU/mL with 15% apple pomace (solid load), pH 9.0, and 1/4 of culture ratio (B. subtilis/B. pumilus) at 30 degrees C after 24h of fermentation period according to the Box-Behnken response surface methodology. Thus, it was concluded that a twofold-increase in pectinase production was achieved by co-culturing of Bacillus spp. compared to our previous monoculture Bacillus study.en_US
dc.identifier.doi10.1007/s12649-017-0142-4
dc.identifier.endpage1249en_US
dc.identifier.issn1877-2641
dc.identifier.issn1877-265X
dc.identifier.issue5en_US
dc.identifier.scopus2-s2.0-85034639968en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1241en_US
dc.identifier.urihttps://doi.org/10.1007/s12649-017-0142-4
dc.identifier.urihttps://hdl.handle.net/20.500.12491/10040
dc.identifier.volume10en_US
dc.identifier.wosWOS:000464765200015en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorUzuner, Sibel
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofWaste And Biomass Valorizationen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectApple Pomaceen_US
dc.subjectPectinasesen_US
dc.subjectSubmerged Fermentationen_US
dc.subjectBacillus Subtilisen_US
dc.subjectBacillus Pumilusen_US
dc.subjectMixed Cultureen_US
dc.titleImprovement of pectinase production by co-culture of Bacillus spp. using apple pomace as a carbon sourceen_US
dc.typeArticleen_US

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