Evaluation of Bio-Rad D-10® and arkray adams HA-8160® HPLC analyzers in HbA1c measurement

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dc.authorid0000-0002-7006-3125
dc.authorid0000-0002-4060-3354
dc.authorid0000-0001-6878-0646
dc.contributor.authorYis, Özgür Mehmet
dc.contributor.authorPehlivan, Mine Büşra
dc.contributor.authorBuğdaycı, Güler
dc.contributor.authorYüce, Neslihan
dc.date.accessioned2021-06-23T18:34:12Z
dc.date.available2021-06-23T18:34:12Z
dc.date.issued2019
dc.departmentBAİBÜ, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.description.abstractGlycosylated hemoglobin A1c (HbA1c) is an important parameter used for the assessment of time-dependent glycemic status and the diagnosis and follow-up of diabetes. This study aimed to evaluate the analytical performance of two HPLC analyzers, the Bio-Rad D-10®, and the Arkray Adams HA-8160®. Accuracy and imprecision studies were conducted, and a method comparison study was performed with 105 samples. Samples were collected on five consecutive days and measured on both analyzers within two hours. Bland-Altman and regression analyses were used for statistical evaluation of the data. Low and high-level within-day CV values were calculated as 1.22% and 0.60% for the Arkray analyzer and 1.2% and 0.30% for the Bio-Rad analyzer, respectively. They were calculated as 1.27% and 2.52% for the Arkray analyzer and 2.32% and 3.44% for the Bio-Rad analyzer, respectively, between days. The within-day CV values for both analyzers were below the limit of 2.5% specified by the International Federation of Clinical Chemistry (IFCC). The bias average for three months was 3.2% for the Arkray analyzer and 1.2% for the Bio-Rad analyzer. The Spearman correlation coefficient (r) was 0.973 (p<0.0001) between the two measurements, and the slope and intercept values calculated in the regression analyses showed the linear relationship and harmony between the two analyzers [R2 = 0.967, (y = 0.860x + 0.604)]. Both HPLC analyzers are shown to be reproducible and accurate for routine biochemistry laboratory use.en_US
dc.identifier.doi10.5455/medscience.2019.08.9084
dc.identifier.endpage956en_US
dc.identifier.issn2147-0634
dc.identifier.issn2147-0634
dc.identifier.issue4en_US
dc.identifier.startpage953en_US
dc.identifier.trdizinid379308en_US
dc.identifier.urihttps://doi.org/10.5455/medscience.2019.08.9084
dc.identifier.urihttps://app.trdizin.gov.tr/makale/TXpjNU16QTRPQT09
dc.identifier.urihttps://hdl.handle.net/20.500.12491/2433
dc.identifier.volume8en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.institutionauthorYis, Özgür Mehmet
dc.institutionauthorBuğdaycı, Güler
dc.language.isoenen_US
dc.relation.ispartofMedicine Scienceen_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectDiabetes Mellitus
dc.subjectHbA1c
dc.subjectHPLC
dc.subjectAnalytical Performance
dc.titleEvaluation of Bio-Rad D-10® and arkray adams HA-8160® HPLC analyzers in HbA1c measurementen_US
dc.typeArticleen_US

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