Surgical pinealectomy in hamsters

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Tarih

2013

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Yayıncı

Nova Science Publishers, Inc.

Erişim Hakkı

info:eu-repo/semantics/closedAccess

Özet

Pineal gland is a small pine-shaped structure located in the epithalamus of the brain and melatonin is the main hormone of this gland that informs the body about the environmental light and dark. Melatonin modulates many physiological functions such as sleep, circadian, visual, cerebrovascular, reproductive, neuroendocrine, neuroimmunological and behavioral functions of the rodents. The removal of the pineal gland (pinealectomy) abolishes the rhythmic endogenous melatonin release and decreases the plasma levels of melatonin significantly. Pinealectomy operation in Syrian hamster may be examined under the four different sections. First, all necessary equipments used in pinealectomy operation is introduced. Second, the hamster is prepared for the surgery. This starts with the anesthesia. In anesthetized hamster, the head is shaved and the surgical area is sterilized. Hamster is placed in a stereotaxic apparatus to stabilize the head during the surgery. Third, the surgery procedures are applied. An incision is made in the scalp. Muscle attachments are removed from the dorsal skull. After drying the skull, an incomplete circular cut is made with a dental drill burr at the ? (lambda) suture and a piece of cranium covering the pineal gland is folded forward anteriorly. Fine-tipped forceps is used to extend into the confluence of the sinuses to grasp and remove the pineal gland. Aspiration is used to control the hemorraging. After removal of the pineal gland, the bone flap is replaced and a small square of absorbable gelatin sponge is applied to the skull surface to help promote clotting. The scalp is closed with stainless steel surgical clips and pinealectomy ends. Finally, the post surgery procedures are applied. The incision is treated with Newskin adhesive to prevent any contamination. The antibacterial and anti-inflammatory drug injections are applied. Hamster is placed on a heating apparatus till it awakes. After awakening, the analgesic drug injections are applied every eight hours for two days. © 2013 Nova Science Publishers, Inc. All rights reserved.

Açıklama

Anahtar Kelimeler

Surgical Pinealectomy

Kaynak

Step by Step Experimental Pinealectomy Techniques in Animals for Researchers

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N/A

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