Molecular analysis of isoniazid, rifampin and streptomycin resistance in Mycobacterium tuberculosis isolates from patients with tuberculosis in Duzce, Turkey

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dc.contributor.authorOzturk, CE
dc.contributor.authorSanic, A
dc.contributor.authorKaya, D
dc.contributor.authorCeyhan, I
dc.date.accessioned2024-09-25T20:01:45Z
dc.date.available2024-09-25T20:01:45Z
dc.date.issued2005
dc.departmentAbant İzzet Baysal Üniversitesien_US
dc.description.abstractThe aim of this study was to use DNA sequencing analysis to analyze the mutations in the most commonly targeted genes (katG, inhA, rpoB, rpsL) in isoniazid (INH)-, rifampin (RIF)- and streptomycin (SM)-resistant Mycobacterium tuberculosis strains obtained from subjects in Duzce, Turkey. Four isolates were found to be INH-resistant, 3 were RIF-resistant and 5 were SM-resistant, out of a total of 52 M. tuberculosis strains. In 3 of the 4 INH-resistant strains, a mutation in the katG gene in codon 315 appeared as AGC -> ACC (Ser -> Thr), and the other INH-resistant strain showed a mutation in the katG gene in codon 314 as ACC -> CCC (Thr -> Pro). There were no mutations in the inhA gene in INH-resistant isolates. Two of the 3 RIF-resistant strains were found to have mutations in the rpoB gene in codon 516 appearing as GAC -> GTC (Asp -> Val), and the other RIF-resistant strain has a mutation in the rpoB gene in codon 531 as TCG -> TTG (Ser -> Leu). These 3 RIF-resistant strains are also INH-resistant. All 5 SM-resistant strains have mutations in the rpsL gene in codon 43 appearing as AAG -> AGG (Lys -> Arg). Thus, we found common gene mutations that bring about the resistance of M. tuberculosis to antituberculosis drugs in all of our isolates from Duzce. To the best of our knowledge, the ACC -> CCC (Thr -> Pro) mutation in the katG gene in codon 314 has not been previously defined.en_US
dc.identifier.endpage312en_US
dc.identifier.issn1344-6304
dc.identifier.issn1884-2836
dc.identifier.issue5en_US
dc.identifier.pmid16249627en_US
dc.identifier.startpage309en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12491/14318
dc.identifier.volume58en_US
dc.identifier.wosWOS:000232992700011en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherNatl Inst Infectious Diseasesen_US
dc.relation.ispartofJapanese Journal of Infectious Diseasesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzYK_20240925en_US
dc.titleMolecular analysis of isoniazid, rifampin and streptomycin resistance in Mycobacterium tuberculosis isolates from patients with tuberculosis in Duzce, Turkeyen_US
dc.typeArticleen_US

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