Alkaloid production from cell suspension cultures obtained from osmotic-stressed callus lines of Catharanthus roseus

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dc.authorid0000-0001-6262-2866
dc.contributor.authorAkçam-Oluk, Esin
dc.contributor.authorDemiray, Hatice
dc.contributor.authorGürel, Ekrem
dc.date.accessioned2021-06-23T18:54:44Z
dc.date.available2021-06-23T18:54:44Z
dc.date.issued2003
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractCatharanthus roseus callus cultures established were first exposed to osmotic stress using polyethylene glycol (9.0%, w/v)(MW 3350) in solid MS medium supplemented with 20 g/l sucrose, 2 mg/l ?-naphtalene acetic acid (NAA) and 3 mg/l 6-benzylamino purine (BAP). Cell suspensions were then initiated from stressed (S) and non-stressed (NS) calli in liquid LS medium. When LS medium was employed, only ajmalicine was detected at 1.308 ?g/ml and 0.405 ?g/ml concentrations for the S and NS cell lines, respectively. None of the other alkaloids normally found in the intact plant, such as vinblastine, vincristine and vindoline was detected at the end of experiments carried out with both media. From these results it can be concluded that, application of the osmotic stress to Catharanthus roseus cell suspension cultures increases ajmalicine production capacity, although it does not trigger the production of other alkaloids involved.en_US
dc.identifier.endpage94en_US
dc.identifier.issn0972-2025
dc.identifier.issue1-2en_US
dc.identifier.scopus2-s2.0-0344585312en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage91en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12491/4557
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-0344585312&partnerID=40&md5=39adc732bd2261d1827364def27c3f20
dc.identifier.volume4en_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorGürel, Ekrem
dc.language.isoenen_US
dc.relation.ispartofPlant Cell Biotechnology and Molecular Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAjmalicine Productionen_US
dc.subjectCatharanthus Roseusen_US
dc.subjectCell Suspension Culturesen_US
dc.subjectOsmotic Stressen_US
dc.titleAlkaloid production from cell suspension cultures obtained from osmotic-stressed callus lines of Catharanthus roseusen_US
dc.typeArticleen_US

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