Expression comparisons of defense-related genes in resistant and susceptible chickpea cultivars in response to Ascochyta rabiei

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dc.authorid0000-0002-3603-2413
dc.authorid0000-0002-3385-2520
dc.authorid0000-0003-2562-4461
dc.contributor.authorPalacıoğlu, Gülsüm
dc.contributor.authorÖzer, Göksel
dc.contributor.authorBayraktar, Harun
dc.date.accessioned2024-09-25T19:56:22Z
dc.date.available2024-09-25T19:56:22Z
dc.date.issued2023
dc.departmentBAİBÜ, Ziraat Fakültesi, Bitki Koruma Bölümü
dc.description.abstractAscochyta blight caused by Ascochyta rabiei is a destructive disease affecting chickpea crops worldwide. Numerous studies have sought to identify resistance genes involved in the defense response against Ascochyta blight, but the function of potential resistant genes across different chickpea genetic backgrounds remains poorly understood. This study evaluated the potential role of 16 genes involved in defense responses in partially resistant (ILC482) and susceptible (Sari98) chickpea cultivars. The leaf samples were collected from 2-week-old seedlings at 6, 12, 24, 48, and 72 h post-inoculation (hpi) with A. rabiei and analyzed for differential expression of defense genes using qRT-PCR. Twelve out of 16 genes were differentially regulated between chickpea cultivars. The qRT-PCR analysis indicated that the expression of defense genes was significantly higher in the partially resistant cultivar than that in the susceptible cultivar. The time point of the highest upregulation ratio for defense genes was variable between the cultivars. The expression of glutathione S-transferase, serine/threonine-protein kinase, WRKY gene (CaWRKY16), and wall associated-receptor kinase genes reached maximum levels in the resistant cultivar at 6 hpi, while antimicrobial peptide precursor (SNAKIN2), polymorphic antigen membrane protein, ethylene receptor-like sequences (CaETR1), and two nucleotide-binding site-leucine-rich repeat genes showed the highest activity at 24 hpi. In particular, CaWRKY16, CaWRKY50, glutathione S-transferase, CaETR1, and wall-associated-receptor kinase genes provided valuable information for assessing chickpea cultivars with different resistance levels. The results indicated that the expression changes of these genes involved in defense pathways play a crucial role in enhanced resistance of chickpea plants against Ascochyta blight and, therefore could be potential candidate genes for future breeding studies.en_US
dc.description.sponsorshipBolu Abant I ? zzet Baysal University Scientific Research Projects Coordination Unit [2016.10.06.1072]en_US
dc.description.sponsorshipThis research was funded by Bolu Abant I ? zzet Baysal University Scientific Research Projects Coordination Unit (2016.10.06.1072) .en_US
dc.identifier.doi10.1016/j.pmpp.2023.102107
dc.identifier.endpage8
dc.identifier.issn0885-5765
dc.identifier.scopus2-s2.0-85165635900en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1
dc.identifier.urihttps://doi.org/10.1016/j.pmpp.2023.102107
dc.identifier.urihttps://hdl.handle.net/20.500.12491/13261
dc.identifier.volume127en_US
dc.identifier.wosWOS:001050371900001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorÖzer, Göksel
dc.institutionauthorid0000-0002-3385-2520
dc.language.isoenen_US
dc.publisherAcademic Press Ltd- Elsevier Science Ltden_US
dc.relation.ispartofPhysiological And Molecular Plant Pathologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzYK_20240925en_US
dc.subjectAscochyta Blighten_US
dc.subjectChickpeaen_US
dc.subjectDefense-Related Gene Expressionen_US
dc.subjectqRT-PCRen_US
dc.subjectResistanceen_US
dc.titleExpression comparisons of defense-related genes in resistant and susceptible chickpea cultivars in response to Ascochyta rabieien_US
dc.typeArticleen_US

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