A novel technology for the one-step detection of prune dwarf virus: Colorimetric reverse transcription loop-mediated isothermal amplification assay

Basit Öğe Kaydı
dc.authorid0000-0002-5836-8030en_US
dc.contributor.authorÇelik, Ali
dc.date.accessioned2023-11-09T07:40:29Z
dc.date.available2023-11-09T07:40:29Z
dc.date.issued2022en_US
dc.departmentBAİBÜ, Ziraat Fakültesi, Bitki Koruma Bölümüen_US
dc.description.abstractA one-step colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed to target the coat protein region of genomic RNA of prune dwarf virus (PDV). WarmStart 2X Master Mix from NEB (New England Biolabs) was used for naked eye observation of positive LAMP reactions, where the changing in color from pink to yellowish indicates a positive result. The detection sensitivity of the new technique was 10-fold higher than that of conventional PCR. The assay's specificity was evaluated against the nucleic acids of potential hosts of PDV, prunus necrotic ringspot virus (PNRSV), and apple mosaic virus (ApMV) from the Ilarvirus genus. The novel RT-LAMP test showed high sensitivity in distinguishing PDV and genome of potential hosts and other Ilarvirus genus members. The RT-LAMP test was proven to be reliable for diagnosis of PDV in infected sweet cherry samples from the field. The specific, sensitive, and quick RT-LAMP test described in this study can be used in laboratories and for PDV surveillance in the field. To best of our knowledge, this is the first report of RT-LAMP detection for PDV.en_US
dc.identifier.citationÇelik, A. (2022). A novel technology for the one-step detection of prune dwarf virus: Colorimetric reverse transcription loop-mediated isothermal amplification assay. Crop Protection, 155, 105910.en_US
dc.identifier.doi10.1016/j.cropro.2022.105910
dc.identifier.endpage8en_US
dc.identifier.issn0261-2194
dc.identifier.issn1873-6904
dc.identifier.scopus2-s2.0-85122956335en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.cropro.2022.105910
dc.identifier.urihttps://hdl.handle.net/20.500.12491/11818
dc.identifier.volume155en_US
dc.identifier.wosWOS:000790734900011en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorÇelik, Ali
dc.language.isoenen_US
dc.publisherElsevier Science Ltden_US
dc.relation.ispartofCrop Protectionen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectRT-LAMPen_US
dc.subjectPDVen_US
dc.subjectRapiden_US
dc.subjectPolymerase-Chain-Reactionen_US
dc.subjectRapid Detectionen_US
dc.subjectMosaic-Virusen_US
dc.titleA novel technology for the one-step detection of prune dwarf virus: Colorimetric reverse transcription loop-mediated isothermal amplification assayen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
ali-celik.pdf
Boyut:
5.12 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin/Full Text
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Bitki Koruma Bölümü Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu