Performance of Rapid Test in Detection of Anti-HCV in Frozen Sera

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dc.contributor.authorMengeloglu, Zafer
dc.contributor.authorBucak, Ozlem
dc.contributor.authorKocoglu, Esra
dc.contributor.authorTas, Tekin
dc.contributor.authorKarabork, Seyda
dc.date.accessioned2024-09-25T19:59:56Z
dc.date.available2024-09-25T19:59:56Z
dc.date.issued2014
dc.departmentAbant İzzet Baysal Üniversitesien_US
dc.description.abstractObjective: Hepatitis C is a common infectious disease throughout the world. Rapid test of hepatitis C, detecting anti-HCV, is a useful test that is easy to perform as well as it may have lack of sensitivity and specificity. In the present study, it was aimed to investigate whether the rapid test of HCV infection can still reveal accurate results in anti-HCV-positive sera frozen for a few years. Materials and Methods: Sera of a total of 100 patients that were determined to be positive for anti-HCV using chemiluminescence technology system (Architect i2000sr, IL, USA) were stored at -200 degrees C for a maximum period of 3.5 years. The sera were thawed and tested again for anti-HCV using the Nanosign rapid test kit (Bioland, Korea) for HCV infection. Results: The positivity rate of the test was 37%. In addition, the rapid test revealed a very low positivity rate as 5.2% in sera with a level of anti-HCV below 10.0 S/CO. In contrast, the positivity rate was 71.4% in samples with high anti-HCV levels. A significant positive correlation was found between positivity levels and anti-HCV levels (r=0.708, p<0.001). No correlation was found between positivity levels and time passed after freezing the samples (r=-0.91, p=0.367). Besides this, no significant difference was observed amongst the groups in terms of the time intervals of freezing (p>0.05). Conclusion: The findings of this study suggest that the rapid test of HCV infection is not reliable in frozen sera with low anti-HCV levels due to the instability of the molecules in the samples, and the time passed after freezing of the sample doesn't change the results of the tests.en_US
dc.description.sponsorshipViral Hepatitis Prevention Societyen_US
dc.description.sponsorshipThis study was supported by Viral Hepatitis Prevention Society.en_US
dc.identifier.doi10.4274/Vhd.48658
dc.identifier.endpage10en_US
dc.identifier.issn1307-9441
dc.identifier.issn2147-2939
dc.identifier.issue1en_US
dc.identifier.startpage8en_US
dc.identifier.trdizinid179972en_US
dc.identifier.urihttps://doi.org/10.4274/Vhd.48658
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/179972
dc.identifier.urihttps://hdl.handle.net/20.500.12491/13973
dc.identifier.volume20en_US
dc.identifier.wosWOS:000217237300004en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherGalenos Yayinciliken_US
dc.relation.ispartofViral Hepatit Dergisi-Viral Hepatitis Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzYK_20240925en_US
dc.subjectRapid testen_US
dc.subjectanti-HCVen_US
dc.subjectfrozen seraen_US
dc.subjectHCVen_US
dc.titlePerformance of Rapid Test in Detection of Anti-HCV in Frozen Seraen_US
dc.typeArticleen_US

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