Performance of Rapid Test in Detection of Anti-HCV in Frozen Sera
Küçük Resim Yok
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Galenos Yayincilik
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Objective: Hepatitis C is a common infectious disease throughout the world. Rapid test of hepatitis C, detecting anti-HCV, is a useful test that is easy to perform as well as it may have lack of sensitivity and specificity. In the present study, it was aimed to investigate whether the rapid test of HCV infection can still reveal accurate results in anti-HCV-positive sera frozen for a few years. Materials and Methods: Sera of a total of 100 patients that were determined to be positive for anti-HCV using chemiluminescence technology system (Architect i2000sr, IL, USA) were stored at -200 degrees C for a maximum period of 3.5 years. The sera were thawed and tested again for anti-HCV using the Nanosign rapid test kit (Bioland, Korea) for HCV infection. Results: The positivity rate of the test was 37%. In addition, the rapid test revealed a very low positivity rate as 5.2% in sera with a level of anti-HCV below 10.0 S/CO. In contrast, the positivity rate was 71.4% in samples with high anti-HCV levels. A significant positive correlation was found between positivity levels and anti-HCV levels (r=0.708, p<0.001). No correlation was found between positivity levels and time passed after freezing the samples (r=-0.91, p=0.367). Besides this, no significant difference was observed amongst the groups in terms of the time intervals of freezing (p>0.05). Conclusion: The findings of this study suggest that the rapid test of HCV infection is not reliable in frozen sera with low anti-HCV levels due to the instability of the molecules in the samples, and the time passed after freezing of the sample doesn't change the results of the tests.
Açıklama
Anahtar Kelimeler
Rapid test, anti-HCV, frozen sera, HCV
Kaynak
Viral Hepatit Dergisi-Viral Hepatitis Journal
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
20
Sayı
1
