Assessing genetic diversity of potato genotypes using inter-PBS retrotransposon marker system

dc.authorid0000-0002-7470-0080en_US
dc.authorid0000-0002-3457-5086en_US
dc.contributor.authorDemirel, Ufuk
dc.contributor.authorTındaş, İlknur
dc.contributor.authorYavuz, Caner
dc.contributor.authorBaloch, Faheem Shehzad
dc.contributor.authorÇalışkan, Mehmet Emin
dc.date.accessioned2021-06-23T19:49:52Z
dc.date.available2021-06-23T19:49:52Z
dc.date.issued2018
dc.departmentBAİBÜ, Ziraat Fakültesi, Tarla Bitkileri Bölümüen_US
dc.description.abstractHaving knowledge on genetic similarity and DNA profile of potato genotypes facilitates a breeder's decision for parent selection and provides accurate variety identification. Fingerprinting and identification of genetic similarity among 151 potato genotypes were achieved using an inter-primer-binding sites (iPBS) retrotransposon marker system. Our study is the first application of iPBS markers for fingerprinting and distinguishing large numbers of Solanum tuberosum genotypes. Initially, 16 potato genotypes were screened using 45 iPBS retrotransposon markers to identify polymorphisms. Seventeen of these primers were selected for fingerprinting the whole set of accessions due to strong, reproducible and polymorphic bands. The 17 iPBS primers produced 290 scorable bands of which 224 were polymorphic. The number of bands per primer ranged from 10 to 26 with an average of 17.1. The number of polymorphic bands per primer was between 6 and 21. The polymorphism percentage per primer ranged from 46.2 to 100.0% with an average of 77.2% per primer. The mean polymorphism information content (PIC) values of iPBS primers varied from 0.12 to 0.31 per primer. Genetic similarity based on Jaccard's coefficient of potato genotypes ranged from 0.61 to 0.93 with an average of 0.73. The data produced herein may be used for selection of appropriate parents and variety description in the future. The findings of the present study suggest that iPBS retrotransposons are powerful and easy DNA markers for fingerprinting the large samples of potato germplasm.en_US
dc.identifier.doi10.1017/S1479262117000041
dc.identifier.endpage145en_US
dc.identifier.issn1479-2621
dc.identifier.issn1479-263X
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85013151862en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage137en_US
dc.identifier.urihttps://doi.org/10.1017/S1479262117000041
dc.identifier.urihttps://hdl.handle.net/20.500.12491/9637
dc.identifier.volume16en_US
dc.identifier.wosWOS:000425490500005en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorBaloch, Faheem Shehzad
dc.language.isoenen_US
dc.publisherCambridge Univ Pressen_US
dc.relation.ispartofPlant Genetic Resources-Characterization And Utilizationen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGenetic Similarityen_US
dc.subjectIPBS Primersen_US
dc.subjectMolecular Characterizationen_US
dc.subjectPotatoen_US
dc.subjectTurkeyen_US
dc.titleAssessing genetic diversity of potato genotypes using inter-PBS retrotransposon marker systemen_US
dc.typeArticleen_US

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