Molecular characterization of cold tolerant germplasm of phaseolus beans with sequence related amplified polymorphism (SRAP) and retrotransposon-based interprimer binding sites (IPBS) markers

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dc.authorid0000-0003-0547-9527
dc.contributor.authorCertel, B. T.
dc.contributor.authorIkten, H.
dc.contributor.authorYilmaz, Y.
dc.contributor.authorKantar, F.
dc.contributor.authorÇiftçi, Vahdettin
dc.contributor.authorGozen, V
dc.contributor.authorTepe, A.
dc.date.accessioned2024-09-25T19:59:47Z
dc.date.available2024-09-25T19:59:47Z
dc.date.issued2023
dc.departmentBAİBÜ, Ziraat Fakültesi, Tarla Bitkileri Bölümü en_US
dc.description.abstractIn this study genetic diversity of 55 Phaseolus sp. beans selected for cold tolerance from different regions of Turkiye was investigated by using sequence related amplified polymorphism (SRAP) and retrotransposon-based interprimer binding sites (iPBS)markers. Four commercially registered cultivars, one accession of Phaseolus coccineus and a tepary bean Phaseaolus acutifolius species were included for comparison. Genomic DNA was isolated from young fresh leaves and PCR reaction was carried out using 30 SRAP (sequence related amplified polymorphism) and 12 iPBS (retrotransposon-based interprimer binding sites) primers. Similarity analyses were performed and dendrograms were produced according to the Unweighted Pair-Group Mean Arithmetic method (UPGMA). In PCR reactions, 331 total and 146 polymorphic bands were produced with 30 SRAP primer combinations. The number of polymorphic bands ranged between 1 and 12 with an average 4.86 polymorphic marker for each primer pair. Twelve iPBS primers produced 156 total bands and 72 of them were polymorphic. The highest polymorphism was obtained with SRAP primer combinations of Me8Em3 and Me7Em14 and iPBS primers 2270, 2394 and 2252. Characterization of germplasm with SRAP and iPBS primers was discussed in relation to cold tolerance, species, source, seed size, seed color and growth type. In conclusion, genetic variability of germplasm of 55 Phaseolus bean species, genotypes and cultivars selected for cold tolerance were effectively assessed by PCR based molecular techniques, SRAP and iPBS. High levels of polymorphism determined in the core collection may be used in breeding programs for the development of cold tolerant superior cultivars.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkiye (TUBITAK) as part of COST Action [FA1306]en_US
dc.description.sponsorshipThis work was financially supported by The Scientific and Technological Research Council of Turkiye (TUBITAK) as part of COST Action No. FA1306 The quest for tolerant varieties: Phenotyping at plant and cellular level) (TOVAG 114 O 806).en_US
dc.identifier.doi10.36899/JAPS.2023.3.0655
dc.identifier.endpage632en_US
dc.identifier.issn1018-7081
dc.identifier.issn2309-8694
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85161280768en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage620en_US
dc.identifier.urihttps://doi.org/10.36899/JAPS.2023.3.0655
dc.identifier.urihttps://hdl.handle.net/20.500.12491/13911
dc.identifier.volume33en_US
dc.identifier.wosWOS:001104092800010en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorÇiftçi, Vahdettin
dc.institutionauthorid0000-0003-0547-9527
dc.language.isoenen_US
dc.publisherPakistan Agricultural Scientists Forumen_US
dc.relation.ispartofJournal of Animal And Plant Sciences-Japsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzYK_20240925en_US
dc.subjectPhaseolus sp. Beansen_US
dc.subjectChilling Toleranceen_US
dc.subjectLandracesen_US
dc.subjectGenetic Diversityen_US
dc.titleMolecular characterization of cold tolerant germplasm of phaseolus beans with sequence related amplified polymorphism (SRAP) and retrotransposon-based interprimer binding sites (IPBS) markersen_US
dc.typeArticleen_US

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