Protein depletion with bacterial cellulose nanofibers

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dc.authoridGOKTURK, ILGIM/0000-0001-7292-7241
dc.authoridTamahkar Irmak, Emel/0000-0002-5913-8333
dc.contributor.authorGokturk, Ilgim
dc.contributor.authorTamahkar, Emel
dc.contributor.authorYilmaz, Fatma
dc.contributor.authorDenizli, Adil
dc.date.accessioned2024-09-25T19:56:25Z
dc.date.available2024-09-25T19:56:25Z
dc.date.issued2018
dc.departmentAbant İzzet Baysal Üniversitesien_US
dc.description.abstractIn this study, we have reported a novel fabrication technique for human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers (MIP-cBCNFs) used for the depletion of HSA selectively from artificial blood plasma for proteomic applications. Molecular imprinting was achieved by using metal ion coordination interactions of N-methacryloyl-(L)-histidinemethylester (MAH) monomer and Cu(II) ions. MAH center dot Cu(II)center dot HSA complex was polymerized with bacterial cellulose nanofibers (BCNFs) under constant stirring at room temperature. The characterization of the MIP-cBCNFs was carried out by FTIR-ATR, SEM, contact angle measurements and surface area measurements. The adsorption experiments of HSA onto the MIP-BCNFs and NIP-BCNFs from aqueous HSA solutions were investigated in a batch system. The selectivity of the MIP-cBCNFs was investigated by using non-template human transferrin (HTR), and myoglobin (Myo). The relative selectivity coefficients of the MIP-cBCNFs were calculated as 4.73 and 3.02 for HSA/HTR and HSA/Myo molecules, respectively. In addition, the depletion of HSA from artificial human plasma was confirmed by SDS-PAGE and 2-D gel electrophoresis. As a result, it has been shown that metal ion coordination interactions contribute to specific binding of template when preparing MIP-cBCNFs for the depletion of HSA with a high adsorption capacity, significant selectivity and reusability.en_US
dc.identifier.doi10.1016/j.jchromb.2018.08.030
dc.identifier.endpage9en_US
dc.identifier.issn1570-0232
dc.identifier.issn1873-376X
dc.identifier.pmid30219335en_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://doi.org/10.1016/j.jchromb.2018.08.030
dc.identifier.urihttps://hdl.handle.net/20.500.12491/13290
dc.identifier.volume1099en_US
dc.identifier.wosWOS:000447579400001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofJournal of Chromatography B-Analytical Technologies in The Biomedical And Life Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzYK_20240925en_US
dc.subjectBacterial cellulose nanofibersen_US
dc.subjectMetal-ion coordinationen_US
dc.subjectMolecularly imprinted polymersen_US
dc.subjectProtein recognitionen_US
dc.subjectProteomicsen_US
dc.titleProtein depletion with bacterial cellulose nanofibersen_US
dc.typeArticleen_US

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