Evaluation of various sunflower (Helianthus annuus L.) genotypes for Agrobacterium tumefaciens-mediated gene transfer

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dc.authorid0000-0001-6262-2866
dc.authorid0000-0001-6262-2866
dc.contributor.authorGürel, Ekrem
dc.contributor.authorKazan, Kemal
dc.date.accessioned2021-06-23T18:54:56Z
dc.date.available2021-06-23T18:54:56Z
dc.date.issued1999
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractIn this work, 10 sunflower (Helianthus annuus L.) genotypes were evaluated for their suitability for Agrobacterium tumefaciens-mediated gene transfer based on detection of a reported gene expression (GUS). A number of factors including the type of shoot-tip explant (split vs. intact), bacterial strain/binary vector combinations and wounding intact shoot-tips by particle bombardment were examined. Genotypes showed significant differences in their transformation efficiencies ranging from 0.0 to 82.7% GUS positive explants, hybrid genotypes being more responsive to Agrobacterium infection than inbred lines. Use of split shoot-tip explants did not increase the transformation efficiency over intact explants but the AGL-1/pKIWI strain/vector combination was more effective than the LBA4404/pTOK233 combination. Wounding explants by particle bombardment prior to inoculations with Agrobacterium had no positive effect on transformation.en_US
dc.identifier.endpage177en_US
dc.identifier.issn1300-008X
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-0040964387en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage171en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12491/4624
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-0040964387&partnerID=40&md5=b5931efe39a7c16e7914fbe5bf1a9e33
dc.identifier.volume23en_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorGürel, Ekrem
dc.institutionauthorKazan, Kemal
dc.language.isoenen_US
dc.relation.ispartofTurkish Journal of Botanyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAgrobacterium Tumefaciensen_US
dc.subjectGene Transferen_US
dc.subjectGenotypesen_US
dc.subjectSunfloweren_US
dc.titleEvaluation of various sunflower (Helianthus annuus L.) genotypes for Agrobacterium tumefaciens-mediated gene transferen_US
dc.typeArticleen_US

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