Alternaria alternata causing inner black rot of lemon (Citrus limon) fruits in Turkey: Genetic diversity and characterisation

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dc.authorid0000-0002-2544-8712en_US
dc.authorid0000-0002-4237-4858en_US
dc.authorid0000-0001-5349-003Xen_US
dc.authorid0000-0002-3385-2520en_US
dc.authorid0000-0002-4917-3813en_US
dc.contributor.authorGüney, İnci Güler
dc.contributor.authorTekin, Fatih
dc.contributor.authorGünen, Tacettin Utku
dc.contributor.authorÖzer, Göksel
dc.contributor.authorDerviş, Sibel
dc.date.accessioned2023-08-11T08:36:50Z
dc.date.available2023-08-11T08:36:50Z
dc.date.issued2023en_US
dc.departmentBAİBÜ, Ziraat Fakültesi, Bitki Koruma Bölümüen_US
dc.description.abstractThe market sales of symptomless lemons in 2020 revealed a persistent and severe internal black rot with browning of the membrane wall and juice sac. To investigate the causal agent, externally symptom-free lemons from Mediterranean locations were sampled from supermarkets, local markets, and bazaars in three southeastern Turkish provinces. Internal black rot symptoms were observed in 13.69% of the 1300 externally symptomless fruits, and Alternaria was isolated from 96.63% of those exhibiting these symptoms. Morphological analysis confirmed the identity of Alternaria alternata in 26 representative isolates collected from various markets and bazaars. To examine genetic diversity or polymorphisms, start codon targeted (SCoT) markers were used. Species identification was based on the sequences of translation elongation factor 1-alpha (TEF 1-alpha), RNA polymerase second largest subunit (RPB2), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). SCoT 29 exhibited the highest amount of polymorphic amplicons, with 18 polymorphic bands scored when compared to the other six scoreable markers. The 26 representative isolates from various markets and bazaars were divided into four clades by SCoT 29. However, multilocus sequence analyses confirmed the identification of a single species, A. alternata. In terms of virulence and the impact of temperature on growth, representative isolates from four SCoT clusters were evaluated. Data suggests that SCoT markers can be used to evaluate virulence-based polymorphism in A. alternata; nonetheless, all clades showed comparable temperature responses, with 25 degrees C being the optimum. There was also no association between individual morphotypes and SCoT clade membership. Due to the lack of obvious signs on lemon fruits, producers, merchants, and consumers are unable to identify black rot, and the widespread presence of A. alternata in lemons may pose a significant risk that must be avoided at all costs.en_US
dc.identifier.citationGüney, İ. G., Tekin, F., Günen, T. U., Özer, G., & Derviş, S. (2023). Alternaria alternata causing inner black rot of lemon (Citrus limon) fruits in Turkey: Genetic diversity and characterisation. Physiological and Molecular Plant Pathology, 125, 101998.en_US
dc.identifier.doi10.1016/j.pmpp.2023.101998
dc.identifier.endpage11en_US
dc.identifier.govdocWOS:000960182600001
dc.identifier.issn0885-5765
dc.identifier.scopus2-s2.0-85150426981en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.pmpp.2023.101998
dc.identifier.urihttps://hdl.handle.net/20.500.12491/11491
dc.identifier.volume125en_US
dc.identifier.wosWOS:000960182600001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorTekin, Fatih
dc.institutionauthorGünen, Tacettin Utku
dc.institutionauthorÖzer, Göksel
dc.language.isoenen_US
dc.publisherAcademic Press Ltd- Elsevier Science Ltden_US
dc.relation.ispartofPhysiological and Molecular Plant Pathologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectFresh Fruit Marketsen_US
dc.subjectLemon Fruitsen_US
dc.subjectBlack Roten_US
dc.subjectScot Markersen_US
dc.subjectTEF1-?en_US
dc.subjectRPB2en_US
dc.subjectGAPDHen_US
dc.titleAlternaria alternata causing inner black rot of lemon (Citrus limon) fruits in Turkey: Genetic diversity and characterisationen_US
dc.typeArticleen_US

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