A study of the effects of metformin, a biguanide derivative, on Annulus Fibrosus and Nucleus Pulposus cells

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dc.authorid0000-0002-5412-8355
dc.authorid0000-0001-5590-0637
dc.authorid0000-0003-2003-6337
dc.authorid0000-0002-1224-442X
dc.authorid0000-0002-5922-2498
dc.contributor.authorKaya, Yasin Emre
dc.contributor.authorKaraarslan, Numan
dc.contributor.authorYılmaz, İbrahim
dc.contributor.authorSirin, Duygu Yaşar
dc.contributor.authorAkalan, Hande
dc.date.accessioned2021-06-23T18:40:36Z
dc.date.available2021-06-23T18:40:36Z
dc.date.issued2020
dc.departmentBAİBÜ, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümüen_US
dc.description.abstractAIM: To investigate the effects of metformin, a drug used widely for the treatment of type 2 diabetes mellitus, on human primary cell cultures prepared from uninjured segment of disc material intervertebral disk tissues. MATERIAL and METHODS: Primary cell cultures were prepared using the tissues of six patients (three males and three females) who had undergone lumbar microdiscectomy and sequestrectomy. Untreated samples served as the control group, and metformintreated samples served as the experimental group. All the samples were evaluated using an inverted light microscope, acridine orange/propidium iodide staining (AO/PI), and a fluorescence microscope. The cytostatic and cytotoxic effects of metformin, which was administered to the samples using a commercial MTT assay kit, were also evaluated. The data obtained were statistically assessed, and the alpha significance value was accepted as less than 0.05. In addition, for the groups’ changes in the expressions of chondroadherin (CHAD), cartilage oligomeric matrix protein (COMP), interleukin-1β (IL-1β) matrix metalloproteinase 7 (MMP-7), and matrix metalloproteinase 19 (MMP-19), genes related to the extracellular matrix synthesis and degradation were determined using gene-specific TaqMan Gene Expression Assays. RESULTS: The administration of the drug adversely affected nucleus pulposus (NP)/annulus fibrosus (AF) cells and extracellular matrix–like structures. This was statistically significant (p<0.05). CONCLUSION: Clinicians should not disregard the adverse effects of metformin, which is used widely in clinical practice, on the components of intervertebral disk tissues.en_US
dc.identifier.endpage441en_US
dc.identifier.issn1019-5149
dc.identifier.issn2651-5032
dc.identifier.issue3en_US
dc.identifier.pmid#YOKen_US
dc.identifier.startpage434en_US
dc.identifier.trdizinid356691en_US
dc.identifier.urihttps://app.trdizin.gov.tr/makale/TXpVMk5qa3hNUT09
dc.identifier.urihttps://hdl.handle.net/20.500.12491/3164
dc.identifier.volume30en_US
dc.identifier.wosWOS:000535739800016en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.institutionauthorKaya, Yasin Emre
dc.language.isoenen_US
dc.relation.ispartofTurkish Neurosurgeryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAnnulus Fibrosusen_US
dc.subjectIntervertebral Disc Tissue
dc.subjectMetformin
dc.subjectNucleus Pulposus
dc.subjectPrimary Human Cell Culture
dc.titleA study of the effects of metformin, a biguanide derivative, on Annulus Fibrosus and Nucleus Pulposus cellsen_US
dc.typeArticleen_US

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