Quartz crystal microbalance based biosensors for detecting highly metastatic breast cancer cells via their transferrin receptors

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dc.authorid0000-0003-3292-8092
dc.authorid0000-0003-3260-1639
dc.authorid0000-0003-4237-5831
dc.authorid0000-0001-5454-7624
dc.contributor.authorAtay, Seda
dc.contributor.authorPişkin, Kevser
dc.contributor.authorYılmaz, Fatma
dc.contributor.authorÇakır, Canan
dc.contributor.authorYavuz, Handan
dc.date.accessioned2021-06-23T19:44:45Z
dc.date.available2021-06-23T19:44:45Z
dc.date.issued2016
dc.departmentBAİBÜ, Gerede Meslek Yüksekokulu, Kimya Ve Kimyasal İşleme Teknolojileri Bölümüen_US
dc.description.abstractA quartz crystal microbalance (QCM) biosensor was developed to detect highly metastatic breast cancer cells by functionalizing the gold sensor surface with transferrin attachment. MDA-MB 231 breast cancer cells with high and MCF 7 cells with low metastatic potential and transferrin expression were used. Serum starved MDA-MB-231 cells were used as control cells. First, poly(2-hydroxyethyl methacrylate) (PHEMA) nanoparticles were prepared by mini-emulsion polymerization of hydroxyethyl methacrylate (HEMA) and ethylene glycol dimethacrylate (EGDMA). Nanoparticles were characterized with a zeta-sizer and then their suspension is dropped on the surface of the QCM and the dried QCM surface was modified further by activation with carbodiimide and transferrin attachment. The QCM biosensor was analyzed by using atomic force microscopy (AFM), ellipsometry, Fourier transform infrared spectrophotometry (FTIR) and contact angle measurements. The cells were applied to the derivatized QCM sensor to investigate the affinity and binding kinetics. The nanoparticles and transferrin were found to form a monolayer on the QCM surface. Binding kinetics were best fitted to the Langmuir-Freundlich adsorption model. The QCM signal was correlated well with the number of transferrin receptors on cells. It is concluded that the QCM biosensor functioning via transferrin receptor interactions may be used to detect breast cancer cells with high metastatic potential.en_US
dc.identifier.doi10.1039/c5ay02898a
dc.identifier.endpage161en_US
dc.identifier.issn1759-9660
dc.identifier.issn1759-9679
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-84952837955en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage153en_US
dc.identifier.urihttps://doi.org/10.1039/c5ay02898a
dc.identifier.urihttps://hdl.handle.net/20.500.12491/9039
dc.identifier.volume8en_US
dc.identifier.wosWOS:000366905700020en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorYılmaz, Fatma
dc.language.isoenen_US
dc.publisherRoyal Soc Chemistryen_US
dc.relation.ispartofAnalytical Methodsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectTransferrin Receptorsen_US
dc.subjectMetastatic Breast Cancer Cells
dc.subjectQuartz Crystal Microbalance Based Biosensors
dc.titleQuartz crystal microbalance based biosensors for detecting highly metastatic breast cancer cells via their transferrin receptorsen_US
dc.typeArticleen_US

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