Antibody purification from human plasma by metal-chelated affinity membranes

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dc.authorid0000-0002-0920-2843en_US
dc.authorid0000-0003-3260-1639
dc.contributor.authorYavuz, Handan
dc.contributor.authorBereli, Nilay
dc.contributor.authorArmutçu, Canan
dc.contributor.authorYılmaz, Fatma
dc.contributor.authorDenizli, Adil
dc.date.accessioned2021-06-23T19:28:46Z
dc.date.available2021-06-23T19:28:46Z
dc.date.issued2012
dc.departmentBAİBÜ, Gerede Meslek Yüksekokulu, Kimya Ve Kimyasal İşleme Teknolojileri Bölümüen_US
dc.description.abstractThe aim of this study is to investigate in detail the feasibility of poly(2-hydroxyethyl methacrylate-N-methacryloyl-(L)-histidine methyl ester), PHEMAH membranes for purification of immunoglobulin G (IgG) from human plasma. PHEMAH membranes were prepared by photo-polymerization technique. Then, Zn2+, Ni2+, Co2+, and Cu2+ ions were chelated directly on the PHEMAH membranes. Elemental analysis assay was performed to determine the nitrogen content and polymerized MAH was calculated as 168.5 mu mol/g. The nonspecific IgG adsorption onto the plain PHEMA membranes was negligible (about 0.25 mg/mL). A remarkable increase in the IgG adsorption capacities were achieved from human plasma with PHEMAH membranes (up to 68.4 mg/mL). Further increase was observed with the metal-chelated PHEMAH membranes (up to 118 mg/mL). The metal-chelate affinity membranes allowed the one-step separation of IgG from human plasma. The binding range of metal ions for surface histidines from human plasma followed the order: Cu2+ > Ni2+ > Zn2+ > Co2+. Adsorbed IgG was eluted using 250 mM EDTA with a purity of 94.1%. IgG molecules could be repeatedly adsorbed and eluted with the metal-chelated PHEMAH membranes without noticeable loss in their IgG adsorption capacity. (C) 2011 Wiley Periodicals, Inc. J Appl Polym Sci 123: 3476-3484, 2012en_US
dc.identifier.doi10.1002/app.34672
dc.identifier.endpage3484en_US
dc.identifier.issn0021-8995
dc.identifier.issn1097-4628
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-82955203851en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage3476en_US
dc.identifier.urihttps://doi.org/10.1002/app.34672
dc.identifier.urihttps://hdl.handle.net/20.500.12491/7063
dc.identifier.volume123en_US
dc.identifier.wosWOS:000298086500032en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorYılmaz, Fatma
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofJournal Of Applied Polymer Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectIMACen_US
dc.subjectAffinity Membranesen_US
dc.subjectImmunoglobulin-Gen_US
dc.subjectPHEMAen_US
dc.titleAntibody purification from human plasma by metal-chelated affinity membranesen_US
dc.typeArticleen_US

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