Determination of Ascochyta blight disease in chickpea using real-time PCR

dc.authorid0000-0002-3603-2413en_US
dc.authorid0000-0002-3385-2520en_US
dc.authorid0000-0002-9747-4004
dc.authorid0000-0003-2562-4461
dc.contributor.authorBayraktar, Harun
dc.contributor.authorÖzer, Göksel
dc.contributor.authorAydoğan, Abdulkadir
dc.contributor.authorPalacıoğlu, Gülsüm
dc.date.accessioned2021-06-23T19:43:28Z
dc.date.available2021-06-23T19:43:28Z
dc.date.issued2016
dc.departmentBAİBÜ, Ziraat Fakültesi, Bitki Koruma Bölümüen_US
dc.description.abstractAscochyta blight is a devastating disease of chickpea caused by Ascochyta rabiei. In this article, we described a real-time PCR assay for the determination and quantification of A. rabiei infection in chickpea tissues and accurate monitoring of disease progression in plant materials inoculated with different inoculation methods. The primer pairs HEF1/HEF2 were designed to anneal to conserved regions of translation elongation factor 1 alpha (EF) gene for specific amplification of 82-bp fragment of A. rabiei based on SYBR Green I technology. The detection limit of assay was determined as 0.1 pg DNA. PCR specificity was confirmed by testing against uninfected chickpea tissues and another fungal species associated with chickpea. The chickpea plants were inoculated by the methods of whole-plant and detached leaflet inoculation. Disease progression in resistant and susceptible cultivars was evaluated at certain time intervals after pathogen inoculation by real-time PCR. The results revealed a good correlation between visual assessments of disease reaction and pathogen quantification in infected chickpea tissues. The target DNA sequence was also amplified from the samples of DNA extracts from artificially infested seed. This technique could provide a useful approach for efficient selection of resistant breeding material in an early stage of infection as an alternative to the visual disease assessment and will be also used for the determination and quantification of A. rabiei infection.en_US
dc.identifier.doi10.1007/s41348-016-0017-0
dc.identifier.endpage117en_US
dc.identifier.issn1861-3829
dc.identifier.issn1861-3837
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-84975044075en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage109en_US
dc.identifier.urihttps://doi.org/10.1007/s41348-016-0017-0
dc.identifier.urihttps://hdl.handle.net/20.500.12491/8782
dc.identifier.volume123en_US
dc.identifier.wosWOS:000381801300002en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorÖzer, Göksel
dc.language.isoenen_US
dc.publisherSpringer Heidelbergen_US
dc.relation.ispartofJournal Of Plant Diseases And Protectionen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAscochyta Blighten_US
dc.subjectChickpeaen_US
dc.subjectDetectionen_US
dc.subjectResistanceen_US
dc.subjectReal-time PCRen_US
dc.titleDetermination of Ascochyta blight disease in chickpea using real-time PCRen_US
dc.typeArticleen_US

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