The use of colorimetric loop-mediated isothermal amplification assay for naked-eye detection of bean common mosaic virus

dc.authorid0000-0002-5836-8030en_US
dc.authorid0000-0001-8021-2844en_US
dc.authorid0000-0003-0490-371Xen_US
dc.authorid0000-0002-3385-2520en_US
dc.contributor.authorÇelik, Ali
dc.contributor.authorMorca, Ali Ferhan
dc.contributor.authorEmiralioğlu, Orkun
dc.contributor.authorYeken, Mehmet Zahit
dc.contributor.authorÖzer, Göksel
dc.contributor.authorÇiftçi, Vahdettin
dc.date.accessioned2023-09-04T05:49:07Z
dc.date.available2023-09-04T05:49:07Z
dc.date.issued2023en_US
dc.departmentBAİBÜ, Ziraat Fakültesi, Bitki Koruma Bölümüen_US
dc.description.abstractBean common mosaic virus (BCMV), one of the most prevalent viral diseases of common beans, has been intensively studied since its initial identification. In this paper, a one-step colorimetric reverse-transcription loop-mediated isothermal amplification (RT-LAMP) method was performed by designing oligonucleotide primers to amplify the gene encoding the coat protein of BCMV. Positive LAMP reactions were observed with the naked eye using WarmStart 2 x Master Mix from NEB, changing from pink to yellowish if the test is positive. The sensitivity of this method was similar to that of conventional PCR. The method was tested on common bean and another virus, bean common mosaic necrosis virus (BCMNV), and it was shown to specifically detect BCMV and not produce false positive from other RNAs. The RT-LAMP test was quite reliable for the diagnosis of BCMV from infected samples, and the precise, sensitive. The cost analysis showed that the development of a less expensive and efficient RT-LAMP assay for BCMV detection is an important step in expanding testing capacity and improving access to accurate testing in resource-limited settings. The test can be utilized in laboratory and in the field for the diagnosis and monitoring of BCMV, and is the first reported use of RT-LAMP for BCMV detection.en_US
dc.identifier.citationÇeli̇k, A., Morca, A. F., Emi̇rali̇oğlu, O., Yeken, M. Z., Özer, G., & Çiftçi, V. (2023). The use of colorimetric loop-mediated isothermal amplification assay for naked-eye detection of bean common mosaic virus. Physiological and Molecular Plant Pathology, 125, 102017.en_US
dc.identifier.doi10.1016/j.pmpp.2023.102017
dc.identifier.endpage7en_US
dc.identifier.issn0885-5765
dc.identifier.scopus2-s2.0-85151514478en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.pmpp.2023.102017
dc.identifier.urihttps://hdl.handle.net/20.500.12491/11637
dc.identifier.volume125en_US
dc.identifier.wosWOS:000976862200001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorÇelik, Ali
dc.institutionauthorEmiralioğlu, Orkun
dc.institutionauthorYeken, Mehmet Zahit
dc.institutionauthorÖzer, Göksel
dc.institutionauthorÇiftçi, Vahdettin
dc.language.isoenen_US
dc.publisherAcademic Press Ltd- Elsevier Science Ltden_US
dc.relation.ispartofPhysiological and Molecular Plant Pathologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBCMVen_US
dc.subjectColorimetricen_US
dc.subjectOne-Stepen_US
dc.subjectRT-LAMPen_US
dc.subjectRapid Detectionen_US
dc.subjectResistanceen_US
dc.titleThe use of colorimetric loop-mediated isothermal amplification assay for naked-eye detection of bean common mosaic virusen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
ali-celik.pdf
Boyut:
2.7 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam metin/Full text
Lisans paketi
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama: