Rapid sensing of Cu+2 in water and biological samples by sensitive molecularly imprinted based plasmonic biosensor

Basit Öğe Kaydı
dc.authoridGOKTURK, ILGIM/0000-0001-7292-7241
dc.authoridSAGLAM, NECDET/0000-0002-5463-8355
dc.contributor.authorSafran, Volkan
dc.contributor.authorGokturk, Ilgrm
dc.contributor.authorDerazshamshir, Ali
dc.contributor.authorYilmaz, Fatrna
dc.contributor.authorSaglam, Necdet
dc.contributor.authorDenizli, Adil
dc.date.accessioned2024-09-25T20:01:32Z
dc.date.available2024-09-25T20:01:32Z
dc.date.issued2019
dc.departmentAbant İzzet Baysal Üniversitesien_US
dc.description.abstractIn this study, copper (II) ion (Cu+2) imprinted poly(hydroxyethyl methacrylate-N-metacryloyl-(L)-cysteine methyl ester [PHEMAC-Cu+2] nanoparticles were synthesized by two-phase mini-emulsion polymerization method and applied to the SPR sensor chip surface for the selective determination of the Cu+2 ions in both aqueous solution, Cu+2-spiked artificial urine and physiological serum samples to investigate the effects of metabolite residues during the analysis. The non imprinted [PHEMAC] nanoparticles were synthesized by applying the same procedure for the [PHEMAC-Cu+2] nanoparticle synthesis except the addition of Cu+2 ions as a control experiment to evaluate the selectivity of the [PHEMAC-Cu+2] nanoparticles. Roughness differences between [PHEMAC-Cu+2] and [PHEMAC] nanoparticles showed that the imprinting process of Cu+2 ions was performed successfully. [PHEMAC-Cu+2] and [PHEMAC] SPR biosensors prepared by attaching nanoparticles onto the surface of sensor chips, were characterized by atomic force microscope, ellipsometer, contact angle measurements. Langmuir adsorption model was found the most applicable model for this affinity system. Results showed that Cu+2 affinity regions on the surface of [PHEMAC-Cu+2] SPR biosensor were homogeneously distributed and have a monolayer structure. Having the high imprinting efficiency with the imprinting factor of 4.74, the [PHEMAC-Cu+2] SPR biosensor was found to show more selectivity towards the target Cu+2 than the non-imprinted [PHEMAC] SPR biosensor. The selectivity studies of [PHEMAC-Cu+2] SPR biosensors for Cu+2 detection were investigated by using Zn+2 and Ni+2 solutions selected as competitor molecules. The results of intraday and interday precision studies were carried out to ascertain the reproducibility of the proposed method and reported as percent relative standard deviation (%RSD) value.en_US
dc.identifier.doi10.1016/j.microc.2019.04.069
dc.identifier.endpage150en_US
dc.identifier.issn0026-265X
dc.identifier.issn1095-9149
dc.identifier.startpage141en_US
dc.identifier.urihttps://doi.org/10.1016/j.microc.2019.04.069
dc.identifier.urihttps://hdl.handle.net/20.500.12491/14189
dc.identifier.volume148en_US
dc.identifier.wosWOS:000474492600018en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofMicrochemical Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzYK_20240925en_US
dc.titleRapid sensing of Cu+2 in water and biological samples by sensitive molecularly imprinted based plasmonic biosensoren_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu