Effect of KATP openers and blockers on AKT and mTOR mRNA levels in the hippocampus and cortex of rats with penicillin induced epilepsy
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Dosyalar
Tarih
2023
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
National Institute of Science Communication and Information Resources - NISCAIR
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Opening or closing of K-ATP channels affects some signaling pathways in the brain. These signaling pathways are thought to be associated with epilepsy. Here, we investigated the effect of K-ATP channel opener and blocker on the AKT mTOR mRNA expressions in the hippocampus and cortex regions in the penicillin model of epilepsy in rats. Four groups were created viz. Control (C), Epilepsy (E), Epilepsy-Opener (E-O) and Epilepsy-Blocker (E-B). Epileptic focus was created by administering penicillin into the brain, and the seizure-related AKT, mTOR mRNA levels were determined by qPCR on days 1(st), 4(th), and 8(th) after the seizure. The AKT mRNA expression levels in the hippocampus were statistically significant (P <0.05) in the E 1(st) day group. mTOR mRNA expression levels were observed to be significantly higher in the E 1(st) day and 8(th) day groups than in control and all opener groups. (P <0.05). mTOR mRNA expression levels were significantly higher in the E-B 1(st) day and 8(th) day group compared to the control and opener groups (P <0.05). AKT mRNA expression levels in the cortex were found to be statistically significantly higher in the E 1(st) day group than in the other groups. mTOR mRNA expression was significantly higher in the E 4(th) day group than in the control group. The results suggest that activation of the AKT/mTOR signaling pathway is effective in the development of epilepsy and that the effect of KATP channels on epilepsy may be via the AKT/mTOR signaling pathway.
Açıklama
This study was financed by the Abant Izzet Baysal University Research Foundation (BAP-grant number: 2019.10.01.1408) .
Anahtar Kelimeler
Antidiabetic, Cellular Stress, Epileptogenesis, Mammalian Target, Tuberous Sclerosis, Signaling Pathway
Kaynak
Indian Journal of Experimental Biology
WoS Q Değeri
Q4
Scopus Q Değeri
Cilt
61
Sayı
9
Künye
Ümit, K., & Hayriye, S. (2023). Effect of KATP openers and blockers on AKT and mTOR mRNA levels in the hippocampus and cortex of rats with penicillin ınduced epilepsy.
