In vitro culture and biological activity of Solanum dulcamara, a medicinal plant

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dc.contributor.authorCansever, E.
dc.contributor.authorTürker, A. U.
dc.date.accessioned2024-09-25T20:01:29Z
dc.date.available2024-09-25T20:01:29Z
dc.date.issued2007
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümü en_US
dc.description55th Annual Congress of the Society-for-Medicinal-Plant-Research -- SEP 02-06, 2007 - Karl Franzens Univ, Graz, AUSTRIAen_US
dc.description.abstractSolanum dulcamara L. (bittersweet) is a medicinal plant that has been used to treat skin diseases, warts, tumors, felons, arthritis, rheumatism, bronchial congestion, heart ailments, ulcerative colitis, eye inflammations, jaundice and pneumonia. A reliable in vitro culture protocol for bittersweet was established. Explants (leaf and petiole segments) were cultured on Murashige and Skoog minimal organics (MSMO) medium with various hormone combinations. Leaf explants formed more shoots than petiole explants. Best shoot proliferation was obtained from leaf explants with 3mg/l BA plus 0.5mg/l IAA and petiole explants with 3mg/l Kinetin plus 0.1mg/l 2,4-D. Regenerated shoots were transferred to rooting media containing different levels of IAA, IBA, NAA or 2,4-D. Most shoots developed roots on medium with 0,5mg/l IBA, 3mg/l IAA and 1mg/l IBA. Rooted plants were transferred to Magenta containers including vermiculate for acclimatization. After 3 weeks, they were transferred to soil. Biological activities of bittersweet extracts were assessed using selected bench-top bioassays (antibacterial and antitumor). Four different kinds of plant extracts were analyzed (IM, FM, IW and FW); methanolic extracts of in vitro-grown (IM) and field-grown (FM) plant materials and aqueous extract of in vitro-grown (IW) and field-grown (FW) plant materials. In general, methanolic extracts of field-grown leaves and stems were the most effective and showed antibacterial activity against Staphylococcus epidermidis, S. aureus, Klebsiella pneumonia, Salmonella typhimurium and Serratia marcescens. In vitro grown plant material showed less antibacterial activity than field grown plant material. All four extracts showed antitumor activity and the percentage inhibition was more than 40% in experiments comparing with control (water). Methanolic extracts showed better antitumor activity than water extracts and field-grown leaves and stems were better than in vitro-grown leaves and stems.
dc.description.sponsorshipSoc Med Plant Resen_US
dc.identifier.endpage881en_US
dc.identifier.issn0032-0943
dc.identifier.issue9en_US
dc.identifier.startpage881en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12491/14150
dc.identifier.volume73en_US
dc.identifier.wosWOS:000249395900265en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherGeorg Thieme Verlag Kgen_US
dc.relation.ispartofPlanta Medicaen_US
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzYK_20240925en_US
dc.titleIn vitro culture and biological activity of Solanum dulcamara, a medicinal planten_US
dc.typeConference Objecten_US

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