Evaluation of Ascochyta resistance in chickpea genotypes with quantitative polymerase chain reaction assay

Basit Öğe Kaydı
dc.authorid0000-0002-3385-2520en_US
dc.authorid0000-0002-3603-2413en_US
dc.authorid0000-0003-2562-4461en_US
dc.contributor.authorÖzer, Göksel
dc.contributor.authorPalacıoğlu, Gülsüm
dc.contributor.authorAydoğan, Abdulkadir
dc.contributor.authorBayraktar, Harun
dc.date.accessioned2024-05-31T10:18:13Z
dc.date.available2024-05-31T10:18:13Z
dc.date.issued2023en_US
dc.departmentBAİBÜ, Ziraat Fakültesi, Bitki Koruma Bölümüen_US
dc.descriptionThis project was supported by the Turkish Scientific and Technological Research Council.en_US
dc.description.abstractAscochyta blight caused by Ascochyta rabiei is a globally important chickpea disease. Host resistance to Ascochyta blight is considered the most practical and effective means of control, but breeding has been hindered by a lack of effective resistance sources, and time-consuming, labour-intensive traditional methods to screen the resistance level of chickpea genotypes. This paper evaluated the progression of pathogen infection and the disease reaction of chickpea genotypes to Ascochyta blight by traditional and molecular methods. The resistance level of 84 chickpea genotypes was assessed by a quantitative polymerase chain reaction assay (qPCR) using a standard curve produced by various known amounts of pathogen DNA and compared with disease scores based on visual assessments 8 days after inoculation. Disease assessments revealed statistically significant differences between the resistance levels of chickpea genotypes, while the quantity of target DNA in the samples inoculated with the pathogen ranged from 0.004 to 83.37 ng. Our results showed a close relationship between the visual assessment of disease severity and the quantification of the target DNA in chickpea genotypes. The genotypes Tub-35, Tub-47, Tub-26, Tub-82, Tub-65 and Tub-69 were classified as highly resistant to Ascochyta blight based on the results of both assays used for screening chickpea genotypes. This qPCR analysis could be used to quantify disease progression in plant tissues and screen chickpea genotypes as a potential alternative to visual assessment of resistance levels in breeding programmes.en_US
dc.description.sponsorshipThis project was supported by the Turkish Scientific and Technological Research Council.; Turkish Scientific and Technological Research Councilen_US
dc.identifier.citationÖzer, G., Palacıoğlu, G., Aydoğan, A., & Bayraktar, H. (2023). Evaluation of Ascochyta resistance in chickpea genotypes with quantitative polymerase chain reaction assay. Journal of Phytopathology, 171(11-12), 620-626.en_US
dc.identifier.doi10.1111/jph.13221
dc.identifier.endpage626en_US
dc.identifier.issn0931-1785
dc.identifier.issn1439-0434
dc.identifier.issue11-12en_US
dc.identifier.scopus2-s2.0-85169792690en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage620en_US
dc.identifier.urihttp://dx.doi.org/10.1111/jph.13221
dc.identifier.urihttps://hdl.handle.net/20.500.12491/12191
dc.identifier.volume171en_US
dc.identifier.wosWOS:001061332300001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorÖzer, Göksel
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofJournal of Phytopathologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.relation.tubitakThis project was supported by the Turkish Scientific and Technological Research Council.; Turkish Scientific and Technological Research Council
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAscochyta Rabieien_US
dc.subjectChickpea Breedingen_US
dc.subjectqPCRen_US
dc.subjectResistance Screening Methodsen_US
dc.subjectReal-Time Pcren_US
dc.subjectDidymella-Rabieien_US
dc.titleEvaluation of Ascochyta resistance in chickpea genotypes with quantitative polymerase chain reaction assayen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
göksel-özerr.pdf
Boyut:
584.23 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam metin/Full text
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Bitki Koruma Bölümü Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu