The effect of pretreating seedlings with bap on direct shoot regeneration from petiole explants of sugar beet (Beta vulgaris L.)

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dc.authorid0000-0001-6262-2866
dc.authorid0000-0003-2379-5363
dc.contributor.authorGürel, Ekrem
dc.contributor.authorTopal, Emel
dc.contributor.authorSongül, Gürel
dc.date.accessioned2021-06-23T18:54:46Z
dc.date.available2021-06-23T18:54:46Z
dc.date.issued2003
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractA 100% seed sterility and 75.6% seed germination rate were achieved when seeds of three sugar beet (Beta vulgaris L.) breeding lines were surface sterilized with a combination of 0.5% Domestos and 5% PPM™. As an in planta treatment with BAP, the sterilized seeds were germinated on MS basal medium containing 1, 3 or 5 mg/l BAP for five weeks and then petiole explants were excised from the seedlings and cultured on two different regeneration media; RGL medium containing 0.5 mg/l BAP and 0.1 mg/l NAA, and RGH medium containing 1.0 mg/l BAP and 0.5 mg/l NAA. Petiole explants taken from seedlings pretreated with 1 mg/l BAP produced significantly more shoots (0.97 shoot per explant) than those taken from seedlings pretreated with 3 or 5 mg/l BAP (0.68 and 0.59 shoot per explant, respectively). The RGL medium containing lower BAP and NAA was significantly more effective in direct shoot production than the RGH medium containing higher BAP and NAA, 1 shoot per explant compared to 0.48, respectively. 21.1% of the regenerated shoots developed roots on medium containing 3 mg/l NAA whereas only 4.1% of the shoots rooted on medium containing 1 mg/l NAA. Genotypic variation was significantly evident for both shoot regeneration and rooting. © 2003 Taylor and Francis Group, LLC.en_US
dc.identifier.doi10.1080/13102818.2003.10817064
dc.identifier.endpage96en_US
dc.identifier.issn1310-2818
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-1242351921en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage89en_US
dc.identifier.urihttps://doi.org/10.1080/13102818.2003.10817064
dc.identifier.urihttps://hdl.handle.net/20.500.12491/4569
dc.identifier.volume17en_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorGürel, Ekrem
dc.institutionauthorTopal, Emel
dc.institutionauthorSongül, Gürel
dc.language.isoenen_US
dc.relation.ispartofBiotechnology and Biotechnological Equipmenten_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectPretreatingen_US
dc.subjectBeta Vulgaris
dc.subjectExplant
dc.titleThe effect of pretreating seedlings with bap on direct shoot regeneration from petiole explants of sugar beet (Beta vulgaris L.)en_US
dc.typeArticleen_US

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